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51.
Kosuke SODA Maya YAMANE Chiharu HIDAKA Kozue MIURA Trang T. H. UNG Hang L. K. NGUYEN Hiroshi ITO Mai Q. LE Toshihiro ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1899
Low and highly pathogenic avian influenza viruses (LPAIVs and HPAIVs, respectively) have been co-circulating in poultry populations in Asian, Middle Eastern, and African countries. In our avian-flu surveillance in Vietnamese domestic ducks, viral genes of LPAIV and HPAIV have been frequently detected in the same individual. To assess the influence of LPAIV on the pathogenicity of H5 HPAIV in domestic ducks, an experimental co-infection study was performed. One-week-old domestic ducks were inoculated intranasally and orally with phosphate-buffered saline (PBS) (control) or 106 EID50 of LPAIVs (A/duck/Vietnam/LBM678/2014 (H6N6) or A/Muscovy duck/Vietnam/LBM694/2014 (H9N2)). Seven days later, these ducks were inoculated with HPAIV (A/Muscovy duck/Vietnam/LBM808/2015 (H5N6)) in the same manner. The respective survival rates were 100% and 50% in ducks pre-infected with LBM694 or LBM678 strains and both higher than the survival of the control group (25%). The virus titers in oral/cloacal swabs of each LPAIV pre-inoculation group were significantly lower at 3–5 days post-HPAIV inoculation. Notably, almost no virus was detected in swabs from surviving individuals of the LBM678 pre-inoculation group. Antigenic cross-reactivity among the viruses was not observed in the neutralization test. These results suggest that pre-infection with LPAIV attenuates the pathogenicity of HPAIV in domestic ducks, which might be explained by innate and/or cell-mediated immunity induced by the initial infection with LPAIV. 相似文献
52.
Mariko TAKAYASU Kouko HAMAMOTO Hiroshi SATOH Toshihiro ICHIJO Toru TAKAHASHI Kazuhisa FURUHAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):261-263
To clarify the effect of renal dysfunction on pharmacokinetics of the prokinetic agent metoclopramide (MCP), we administered intravenously 0.4 mg/kg MCP to healthy calves and calves subjected to right kidney vessel ligation (ligation) without or with a subsequent left nephrectomy (ligation plus removal). Plasma MCP concentration, glomerular filtration rate (GFR) and plasma prolactin level were measured by liquid chromatography-tandem mass spectrometry, simplified equation using iodixanol and enzyme-linked immunosorbent assay, respectively. Only in calves with ligation plus removal, plasma MCP concentrations were increased significantly 6, 8 and 12 hr after injection, showing that a negative correlation was observed between the plasma MCP concentrations and GFR value. A tendency to increase in plasma PRL concentration was noted also in these calves. In conclusions, plasma MCP concentrations depend on the GFR mode in calves, and its critical GFR value was estimated. 相似文献
53.
Toyokazu KOBAYASHI Hiroshi KOIE Arisa WATANABE Arisa INO Kazuya WATABE Mujo KIM Kiichi KANAYAMA Kazuya OTSUJI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):503-506
We examined the effects of chicken egg hydrolysate (also known as “bone peptide” or BP)
on bone metabolism in 5- to 8-month-old orchidectomized dogs. The bone formation marker
serum bone alkaline phosphatase (BAP) and the bone resorption marker urine
deoxypyridinoline (DPD) were used as indicators to measure changes in bone metabolism. The
following results were observed that Serum BAP was higher in dogs fed BP-enriched food
throughout the clinical investigation. Serum BAP was statistically significantly higher in
dogs fed BP-enriched food than in dogs fed non-BP-enriched food at 2 months after
orchidectomy. This suggests that BP promoted bone formation immediately after
orchidectomy. 相似文献
54.
Nobukazu SAISHU Kazutaka MORIMOTO Hiroshi YAMASATO Hiroichi OZAKI Toshiyuki MURASE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1037-1042
Thirty-eight Aerococcus viridans isolates were obtained from milk from
478 cows with clinical mastitis in a farm during the periods between November 2011 and
February 2012, and between December 2012 and March 2013. Additional isolates were obtained
from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding
materials. The processed manure was later used to cover the floor of the stalls in barns
as bedding materials. The temperatures recorded in the composted and processed manure were
not as high as those generally observed during satisfactory composting. To reveal the
association of A. viridans in manure-related products with intramammary
infection in cows, isolates were characterized by their DNA fragment patterns as
determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility
testing. Isolates obtained from milk, processed manure and bedding materials had identical
DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from
milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to
clindamycin, whereas virtually all the isolates were susceptible to 12 other
antimicrobials including cefalosporins that have been used to treat bovine mastitis in
Japan. In vitro, three A. viridans isolates from milk
and an isolate from processed manure survived for 3 hr in Good’s buffer (pH 9) at high
temperature (50°C). The results suggest that the processed manure and bedding materials in
this farm were possible sources of A. viridans that caused infection in
the cows with mastitis. 相似文献
55.
Weak activity of UDP-glucuronosyltransferase toward Bisphenol analogs in
mouse perinatal development
Risa YABUSAKI Hidetomo IWANO Sumito TSUSHIMA Nanako KOIKE Naoko OHTANI Kentaro TANEMURA Hiroki INOUE Hiroshi YOKOTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1479-1484
Bisphenol A (BPA) is a widely used industrial chemical that disrupts endocrine function.
BPA is an endocrine disrupting chemical (EDC) that has been demonstrated to affect
reproductive organ development, brain development, metabolic disease and post-natal
behavior. Accordingly, Bisphenol analogs, Bisphenol F (BPF, bis (4-hydroxyphenyl) methane)
and Bisphenol AF (BPAF, 4,4-hexafluoroisopropylidene) diphenol) are used as replacements
for BPA. BPA is mainly metabolized by UDP-glucuronosyltransferase (UGT), UGT2B1, but this
effective metabolizing system is weak in the fetus. In the present study, we demonstrated
that hepatic UGT activity toward BPAF was very weak, in comparison with BPA and BPF, in
the fetus, pups and dams. Conversely, hepatic UGT activity toward BPF was very weak in the
fetus and newborn pups, and was increased to the same level as BPA post-partum. In
conclusion, BPAF possibly tends to accumulate in the fetus, because of weak metabolism
during the perinatal period, suggesting that the metabolism of individual Bisphenol
analogs requires assessment to properly gauge their risks. 相似文献
56.
57.
Ohya T Araki H Sueyoshi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):837-840
We examined the usefulness of PCR-based restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR combined with a newly devised rapid biochemical test using microplates for identifying weakly beta-hemolytic intestinal spirochetes (WBHIS) isolated from pigs. WBHIS strains showing atypical biochemical characteristics were decisively identified at the species level by PCR-RFLP and species-specific PCR. Identification of WBHIS at the species level in routine diagnostic work will certainly contribute to clarifying the pathogenicity of WBHIS. 相似文献
58.
59.
The heritability of tolerance of wheat F3 lines to competition from annual ryegrass (Lolium rigidum), and its relationship to morphological traits, were determined using crosses between wheat cultivars with good and poor
competitive ability. Forty F2-derived F3 lines from a cross between two late flowering varieties (Machete × Spear) and 40 from a cross between early flowering varieties
(Wilgoyne × Gutha) were grown in the field with and without annual ryegrass. There was significant genetic variation between
lines within each of the two populations in a number of aspects of plant growth and development, including yield in monoculture.
The estimates of heritability for % yield loss due to competition were 0.25 and 0.57 in the two crosses respectively, indicating
that selection for high tolerance to competition in the F3 generation or later should be effective. Fairly strong relationships between height and % yield loss and between leaf length
and % yield loss suggest that these may be useful auxiliary traits when selecting for low % yield loss. However, differences
between crosses in the magnitude and sign of genetic and phenotypic correlation between traits indicate that competitive ability
is a complex character influenced by many factors.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
60.
Naonori Hamato Osamu Kotake Nagaaki Ono Hiroshi Kurashima Masaru Nakano Yuzuri Iwamoto Yoshihiko Takahashi 《Breeding Science》2014,64(4):427-434
‘Niigata S3’ is a new strawberry (Fragaria × ananassa Duch.) cultivar that is early flowering and possesses high soluble solid content and good coloration. It was selected from a cross between Kei812 (seed parent) and ‘Asuka-Ruby’ (pollen parent). The first harvest date of ‘Niigata S3’ was December 27, 34 days earlier than ‘Echigohime’ and 9 days earlier than ‘Asuka-Ruby’ (means of 2007 and 2008). The marketable yield of ‘Niigata S3’ was 85% of ‘Echigohime’, 107% of ‘Asuka-Ruby’, while the early yield was 145% of ‘Echigohime’, 85% of ‘Asuka-Ruby’ (based on 2007 and 2008 means). The shape of the fruit is long conical, and its skin color medium-red. The fruit skin hardness of ‘Niigata S3’ was 31.5 g/mm2, which was harder than ‘Echigohime’, and its average soluble solid content was 11.4%, which was higher than the values for ‘Echigohime’ and ‘Asuka-Ruby’ (2008). Furthermore, ‘Niigata S3’ did not bear apical overripe fruit. This new cultivar is adaptable to the climatic conditions of Niigata, as well as other regions that experience low winter temperatures and insolation. 相似文献