Perching and floor laying by domestic hens: Experimental results and their commercial application

1. The effect of perching experience on use of ground level nest boxes was tested in medium hybrid hens. Twelve out of 14 hens (86%) reared without perches laid their first egg on the floor when isolated with ground level nests. Only 4 out of 19 hens (21%) reared with perches laid on the floor. Medium hybrids with experience of only one level apparently have difficulty even stepping up to another level.

2. The age at which perches should be introduced to minimise floor laying was investigated in a second experiment with medium hybrids. Five groups had perches provided at 4, 8, 12, 16 and 20 weeks respectively. Floor laying in the first group varied between 0 and 10%. The proportion of floor eggs laid by the other groups was between 31 and 100% initially, falling to between 23 and 43% by the end of the experiment. Provision of perches as early as possible is recommended.

3. In a commercial trial, perches were provided in a rearing pen of 2600 broiler grandparents. A control pen of 2130 birds had no perches during rearing. At 30 weeks old, floor laying in the experimental flock was 5% of total production, compared to 11% in the control flock. The effect of providing perches during rearing on subsequent floor laying may be of considerable commercial significance.     

Factors Affecting Nest Success and Predator Assemblage of Breeding Birds in Semiarid Grasslands

Woody encroachment has influenced wildlife distributions and, thus, predator-prey dynamics, for many taxa in North American grasslands. In 2015 and 2016, we examined how vegetative characteristics influenced avian nest predator assemblages and nest predation rates in semiarid grasslands of south Texas, where encroachment of woody plant species is common. We monitored 253 nests of 17 bird species and deployed infrared cameras at 107 nest sites within four vegetation types at our study sites. We also used data from a concurrent, multispecies monitoring project within our study area to assess predator activity within these same vegetation types. We divided bird species into four nest types based on nest shape and size (i.e., small, medium, and large cup-shaped nests and exposed nests with little structure). We then used logistic regression to examine relationships between shrub cover, concealment, and distance to edge and the probability of nest success and predation by snakes. We observed a significant decrease in nest success of our medium-sized, cup-shaped nest type when shrub cover increased at the nest site, indicating small increases in shrub cover (≈ 10%) could have substantial impacts on birds using this nest type. Snakes were our primary predator at camera-monitored nests (59%), and snake activity increased by 6.7% with every 10% increase in shrub cover at the nest site. Mesomammalian and large mammalian predators were most active in vegetation types predominated by herbaceous cover, small mammals were most active in vegetation types predominated by woody cover, and snake activity was highly variable. Predator activity did not reflect predator identity at camera-monitored nests, suggesting that potential nest predator activity may not accurately reflect the risk of nest predation. Results of our study will help inform management of bird species using semiarid grasslands affected by woody encroachment and offer recommendations for improved nest success.     

Additive and synergistic effects of land cover,land use and climate on insect biodiversity

Context

We address the issue of adapting landscapes for improved insect biodiversity conservation in a changing climate by assessing the importance of additive (main) and synergistic (interaction) effects of land cover and land use with climate.

Objectives

We test the hypotheses that ant richness (species and genus), abundance and diversity would vary according to land cover and land use intensity but that these effects would vary according to climate.

Methods

We used a 1000 m elevation gradient in eastern Australia (as a proxy for a climate gradient) and sampled ant biodiversity along this gradient from sites with variable land cover and land use.

Results

Main effects revealed: higher ant richness (species and genus) and diversity with greater native woody plant canopy cover; and lower species richness with higher cultivation and grazing intensity, bare ground and exotic plant groundcover. Interaction effects revealed: both the positive effects of native plant canopy cover on ant species richness and abundance, and the negative effects of exotic plant groundcover on species richness were greatest at sites with warmer and drier climates.

Conclusions

Impacts of climate change on insect biodiversity may be mitigated to some degree through landscape adaptation by increasing woody native vegetation cover and by reducing land use intensity, the cover of exotic vegetation and of bare ground. Evidence of synergistic effects suggests that landscape adaptation may be most effective in areas which are currently warmer and drier, or are projected to become so as a result of climate change.

     

Assessment of viral presence in semen and reproductive function of frozen-thawed spermatozoa from Pallas' cats (Otocolobus manul) infected with feline herpesvirus.

Although herpesviruses are known to contaminate the semen of several mammalian species, the occurrence of feline herpesvirus type 1 (FHV-1) in semen of infected cats has not been reported. Our objectives in this study were to investigate the presence of FHV-1 DNA in seminal fluid and frozen-thawed spermatozoa from FHV-1 infected Pallas' cats (Otocolobus manul) and assess the functionality of their frozen-thawed spermatozoa in vitro. Over a 3-yr period, semen (n = 33 ejaculates) was collected periodically via electroejaculation from four Pallas' cats chronically infected with FHV-1. Spermic ejaculates were frozen by pelleting on dry ice and stored in liquid nitrogen. After thawing, sperm motility and acrosome status were assessed over time during in vitro culture. For vitro fertilization (IVF), viable domestic cat (Felis silvestris catus) oocytes were inseminated with frozen-thawed Pallas' cat spermatozoa and evaluated for embryo cleavage. For FHV-1 polymerase chain reaction (PCR) analysis, DNA was extracted from seminal fluid, frozen-thawed spermatozoa, inseminated oocytes, heterologous IVF embryos, and conjunctival biopsies and analyzed for presence of a 322-base pair region of the FHV-1 thymidine kinase gene. Immediately post-thaw, sperm motility and percentage of intact acrosomes were decreased (P < 0.05) compared to fresh samples, and declined further (P < 0.05) during culture. However, all frozen-thawed IVF samples were capable of fertilizing domestic cat oocytes (overall, 46.1 +/- 6.0% cleavage). PCR analysis did not identify FHV-1 DNA in any reproductive sample despite the repeated detection of FHV-1 DNA in conjunctival biopsies. These results suggest that semen collected from Pallas' cats infected with FHV-1 does not contain cell-associated or non-cell-associated virus and that frozen-thawed spermatozoa exhibit adequate function for potential genetic rescue with minimal risk of FHV-1 transmission.     

Growth and survival of the mycoparasiteConiothyrium minitans on lettuce leaves in contact with soil in the presece or absence ofSclerotinia sclerotiorum

Lettuce leaves co-inoculated withSclerotinia sclerotiorum andConiothyrium minitans and controls were placed on, or buried in, soil for a period of two weeks to study development and survival ofC. minitans. OnS. sclerotiorum-infected leaves on the soil surface, the number of colonies ofC. minitans recovered was about 40% of the number of pycnidiospores applied. When buried in the soil there was a reduction to about 2% of the spores applied. WhenC. minitans was applied on healthy lettuce leaves, which were subsequently placed on or in soil, the recovery was about 4%. It is argued that these figures indicate multiplication ofC. minitans onS. sclerotiorum-infected lettuce leaves on the soil, and good survival in all other cases.