Study on the performance enhancing effect of rare earth elements in growing and fattening pigs

A feeding study was performed to investigate possible performance enhancing effects of rare earth elements (REE) in growing and fattening pigs, as well as their influence on the blood serum biochemical changes and the accumulation of REE in the organs of pigs treated with a REE diet for a longer time period. Fourteen crossbred piglets (Deutsche Landrasse × Piétrain) were allotted to two dietary treatments: a control group and the REE-treated group which was supplemented with 300 mg of an REE mixture per kg feed. The REE mixture contained mainly chlorides of lanthanum (La), cerium (Ce) and praseodymium (Pr). The whole feeding period consisted of a 2 months ad libitum feeding period M-I and a 1 month restricted feeding period M-II. It was found that in comparison with the control group, the REE group had a better daily body weight gain of 19% (p   < 0.05) in the period M-I and 12% in the period M-II; the REE group also had a better feed conversion ratio of 11% in period M-I and 3% (p   > 0.05) in the period M-II. The REE had no significant (p   > 0.05) influence on blood serum thyroxine (T₄), aspartate-amino-transferase (AST), alanine-amino-transferase (ALT), alkaline-phosphatase (AP), total cholesterol, triglyceride, total protein, albumin, glucose, Ca, P, Na, K and Cl. However, serum triiodothyronine (T₃) in the REE group was significantly (p   < 0.01) lower than that in the control group. The accumulation rate of La and Ce in the muscle, liver and kidneys was very low after feeding the REE diet for 3 months. The study indicates the possibility of using rare earth elements as safe and inexpensive alternative performance enhancers for pig production.     

江西地区鸭疫里氏杆菌的鉴定及药敏试验

从江西部分地区的25份临床疑似鸭疫里氏杆菌(RA)病例中分离到19株RA。生化特性测定结果表明,19个RA菌株的过氧化氢酶、尿素酶均为阳性,除1株发酵葡萄糖、1株发酵麦芽糖外均不发酵各种糖类,5个RA菌株能液化明胶,15个RA菌株氧化酶阳性,不产生H2S,不还原硝酸盐,吲哚试验、MR试验、VP试验均阴性;80%的菌株对头孢曲松、头孢哌酮、头孢唑啉、罗红霉素等高度敏感,而对阿米卡星、庆大霉素、复方新诺明、卡那霉素、青霉素等耐药。     

家蚕丝质性状选择效果研究

本文采用系统选择、杂交改良等方法。对解舒、净度、强伸力、茧丝长等家蚕主要丝质性状进行了研究。结果表明,解舒、净度、茧丝长系统选择与杂交改良是有效的;对解舒率和茧丝长进行严格综合系统选择,在一定程度上能克服解舒率与茧丝长的负相关关系;系统选择法所建立的解舒好系与解舒差系能稳定遗传;解舒率与强力、伸长度成正相关,但相关程度很低,一代杂种的强力高于原种。     

Expression and functional analysis of the Follistatin-like 3 (FSTL3) gene in the sheep ovary during the oestrous cycle

Follistatin-like 3 (FSTL3) is a regulator of cellular apoptosis and was previously identified via RNA-Seq to be associated with follicular development in mammalian ovaries. However, the mechanism underlying the FSTL3 regulation of oestrus in sheep remained poorly understood. In this study, the oestrogen (E2) and progesterone (P4) concentrations in blood were detected, and the expression level and functional analysis of FSTL3 in the ovary were studied during the different reproductive stage in Aohan fine wool sheep (seasonal breeding breed in China). The concentrations of E2 and P4 at the anestrus were significantly lower compared to dioestrus, proestrus and oestrus stages. Higher expression levels of FSTL3 were observed in the sheep ovary, hypothalamus, and thyroid. During different reproductive stages, higher expression levels were found during the stages of dioestrus and proestrus, while lower levels were found during the oestrus and anestrus stages. Functional analysis of FSTL3 was performed in primary granulosa cells (GCs) of sheep. The concentration of E2 increased significantly after RNAi interference of FSTL3, while the P4 level decreased. FSTL3 can decrease P4 levels, which might be involved in mediating oestrous cycle in sheep.     

不同氧化方法对羽毛粉中胱氨酸生成磺基丙氨酸效果的影响

采用均匀设计方法研究了过甲酸法、过乙酸法氧化羽毛粉中胱氨酸为磺基丙氨酸的效果，结果表明，两种方法的效果无显著差异（Ｐ＞０．０５）。从实用经济角度来考虑，以使用过乙酸氧化法为宜。使用此法的最适条件为：过乙酸用量（Ｘ１）０．１ｍＬ／ｇ羽毛粉，过乙酸组成（Ｘ２）（过氧化氢∶乙酸）１∶８；过乙酸的浓度（Ｘ３）９０％；氧化温度１１０℃（Ｘ４）；氧化时间（Ｘ５）８ｈ。在此条件下，磺基丙氨酸最佳得率为８．０２％。氧化条件（Ｘ）与生成磺基丙氨酸（Ｙ）之间的回归方程：ｙ＝０．９４２２＋０．０６８６Ｘ１＋０．５７８７Ｘ２＋０．００４９Ｘ３＋０．００５２Ｘ４＋０．２９５９Ｘ５。     

安徽省一起非洲猪瘟疫情的暴发调查

2018年9月,安徽省铜陵市某猪场暴发非洲猪瘟疫情。为查明疫情可能的来源、波及范围,继而提出有针对性的预防控制建议,对该猪场的饲养管理、猪群发病现状以及相关流行病学关联场点进行了现场调查。调查发现,此次暴发可能由饲喂泔水引起。由此建议:暂时关闭辖区内生猪屠宰场,并进行彻底消毒;停止疫情相关区域生猪及其产品调运;监测受威胁区猪场,做好生物安全防护;停止饲喂泔水,并做好餐厨剩余物的无害化处理工作。     

疑似犬传染性支气管炎暴发的病例报告

<正>近日,某基地及周边60余头工作犬暴发了以强烈阵咳、呕吐白色粘液、发热、传播迅速为主要特征的急性呼吸道疾病。时值甲型H1N1流感高发季节,我们及时采取综合治疗措施,在较短的时间得到有效控制,并     

重庆蚕业产业布局及发展模式探索——浅析"巩固渝西、提升库区、发展渝东南"产业发展战略

蚕业是重庆市的传统优势产业和特色产业,也是全市农村经济和农民致富的重要骨干项目和出口创汇的骨干产品,为国民经济发展做出过重要贡献.<重庆市茧丝绸"十一五"发展规划>明确提出未来5年蚕桑产业发展的战略方针是"巩固渝西、提升库区、发展渝东南".笔者从重庆的自然资源优势、社会经济发展、蚕业区域布局等方面对这一产业发展战略的科学性、内涵、模式、推进措施等进行了思考、探索,以此提供给各地在发展蚕业和制定规划中参考.     

盐分浓度对星星草种子萌发的影响

星星草（Ｐｕｃｃｉｎｅｌｌｉａｔｅｎｕｉｆｌｏｒａ）种子经不同浓度ＮａＣｌ溶液处理结果表明；与对照相比，低于０．６ｇ／１００ｍｌ的盐浓度对种子萌发有促进作用；介于０．７ｇ／１００ｍｌ—３．６ｇ／１００ｍｌ间，种子的萌发势和累积发芽率显著下降，但尚不影响其萌发潜势，解除盐分限制后，还可正常萌发；高于３．７ｇ／１００ｍｌ后，不仅抑制种子萌发，并对其萌发潜势有显著影响。     

猪流行性腹泻病毒实时荧光定量RT-PCR检测方法的建立

根据Gen Bank报道的N基因高度保守核苷酸序列,设计并合成一对引物。上下游引物与Gen Bank中登录的153株猪流行性腹泻病毒(PEDV)N基因全长序列匹配度分别是100%和97%。以本实验室分离流行毒株为模板,利用SYBR Green I荧光染料法进行RT-PCR扩增,获得扩增产物构建重组质粒作为阳性对照,建立检测猪流行性腹泻病毒核酸的方法。同一样品进行3次重复试验,变异系数0.9%。通过对临床样品进行检测和测序验证,核酸检测结果中的阳性样品准确率为100%。本研究所建立的荧光定量PCR检测方法具有快速、灵敏、准确等优点,可用于临床PEDV的检测及分子流行病学调查。