Marker-assisted increase of genetic diversity in a double-low seed quality winter oilseed rape genetic background

Generation of novel genetic diversity for maximization of heterosis in hybrid production is a significant goal in winter oilseed rape breeding. Here, we demonstrate that doubled haploid (DH) production using microspore cultivation can simultaneously introgress favourable alleles for double‐low seed quality (low erucic acid and low‐glucosinolate content) into a genetically diverse Brassica napus genetic background. The DH lines were derived from a cross between a double‐low quality winter rapeseed variety and a genetically diverse semisynthetic B. napus line with high erucic acid and high glucosinolates (++ quality). Twenty‐three low‐glucosinolate lines were identified with a genome component of 50–67% derived from the ++ parent. Four of these lines, with a genome component of 50–55% derived from the ++ parent, also contained low erucic acid. Heterosis for seed yield was confirmed in test‐crosses using these genetically diverse lines as pollinator. The results demonstrate the potential of marker‐assisted identification of novel genetic pools for breeding of double‐low quality winter oilseed rape hybrids.     

U.l.v. Application of Bioresmethrin by Helicopter for Musca Control in Saudi Arabia

Abstract

A six hundred hectare township in Saudi Arabia was sprayed by a Micronair equipped helicopter with approximately 11 g of bioresmethrin in 550 ml solvent per hectare, applied in the early morning, on one occasion only, in order to evaluate the technique for fly control. The kill of caged wild flies (Musca domestica nebulo F.) in the open was complete. However, sticky traps and Scudder grill counts indoors indicated only 69% and 72% reduction of fly density. Although coverage by aerial application was found to be accurate, penetration into such areas as covered markets was poor, and therefore the method can only be recommended as an adjunct to ground based operations.     

Effect of rhizosphere microbiome on different crop growing fields in various rice cultivars and its molecular approaches for sustainable agro-ecosystem

Journal of Crop Science and Biotechnology - The present investigation was carried out to investigate the effects of rhizosphere bacteria on growth and rice yields. Also, isolation and...     

Radioreceptor assay of human chorionic gonadotropin: detection of early pregnancy

A rapid, sensitive, and specific radioreceptor assay for the determination of human chorionic gonadotropin and luteinizing hormone in plasma is described. Plasma membranes of bovine corpora lutea of early pregnancy, which bind biologically active labeled human chorionic gonadotropin, have been used as receptor. Pregnancy could be detected by assaying the gonadotropin in plasma samples obtained from day 6 to 8 after conception.     

Electronic structure of mott insulators studied by inelastic X-ray scattering

The electronic structure of Mott insulators continues to be a major unsolved problem in physics despite more than 50 years of research. Well-developed momentum-resolved spectroscopies such as photoemission or neutron scattering cannot probe the full Mott gap. High-resolution resonant inelastic x-ray scattering revealed dispersive charge excitations across the Mott gap in a high-critical temperature parent cuprate (Ca(2)CuO(2)Cl(2)), shedding light on the anisotropy of the Mott gap. These charge excitations across the Mott gap can be described within the framework of the Hubbard model.     

Thermostability of Barley Malt Proteases in Western Canadian Two‐Row Malting Barley

During the malting process, barley is germinated via a carefully controlled procedure so that its components are degraded to sugars, amino acids, and other low molecular weight compounds that can be used for subsequent fermentation. One of the most important of these processes is the hydrolysis of proteins into peptides and amino acids. During seed germination, proteases hydrolyze insoluble reserve proteins into soluble peptides that are subsequently hydrolyzed into free amino acids. During kilning, green malt is initially air dried at 40–60°C, and then the temperature is gradually increased to 85–95°C. Although most proteases are denatured during kilning, the malt contains a small proportion of heat‐stable protease enzymes able to further break down protein in the subsequent mashing process. In this study, protocols were developed and standardized to measure the activity of different proteases. These protocols were then used to study protease thermostability in Canadian two‐row spring malting barley lines. We found a wide range in protease activity under controlled conditions. Upon heat treatment, several lines exhibited significant protease thermostability. These thermostable enzymes were purified by ammonium sulfate precipitation and Sephadex columns for further study.     

从陈旧血液提取基因组DNA的改进方法

目的:建立一种快速、有效、陈旧血样基因组DNA的提取方法。方法:采用SDS,EDTA,Tris,蛋白酶K作细胞裂解剂裂解细胞和细胞核,用Tris饱和酚和氯仿:异戊醇(24:1)直接提取基因组DNA。结果该方法提取陈旧血样基因组DNA的纯度高。结论:本法简便、有效,适用于批量陈旧血样的处理。     

Clinical,virological, imaging and pathological findings in a SARS CoV-2 antibody positive cat

This paper reports a presumptive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in a cat. A cat with respiratory disease living with three individuals with coronavirus disease 2019 showed bilateral ground-glass opacities in the lung on X-ray and computed tomography. The clinical swabs were negative for SARS-CoV-2 RNA, but the serum was positive for SARS-CoV-2 antibodies. Interstitial pneumonia and prominent type 2 pneumocyte hyperplasia were noted on histopathology. Respiratory tissues were negative for SARS-CoV-2 RNA or antigen, but the cat was positive for feline parvovirus DNA. In conclusion, the respiratory disease and associated pathology in this cat could have been due to exposure to SARS-CoV-2.     

Functional Properties of Rice Bran Protein Isolate at Different pH Levels

The functional properties of proteins from Tarom and Shiroodi cultivars were determined and compared with technological aspects of food and nutraceutical applications. Shiroodi has higher protein content than Tarom, and the yields of protein obtained were 72.88 and 66.36%, respectively. Nitrogen solubilities of rice bran protein of Tarom were more than Shiroodi at all pH levels. In addition, higher solubility was found in acidic or alkaline conditions. Although the rice bran proteins had lower emulsifying properties than bovine serum albumin, they had similar foaming properties in comparison with egg white. Tarom isolates had a significantly higher solubility, emulsifying property, and foaming stability and greater surface properties than Shiroodi isolates. The results showed the surface hydrophobicities of rice bran protein were greater than casein and ovalbumin and lower than other proteins such as bovine serum albumin. Water and oil absorption capacities were 1.03 and 1.66 for Tarom and 87.3 and 75.3 for Shiroodi, respectively. The bulk densities of Tarom and Shiroodi were also 0.55 and 0.53 g/mL, which make them suitable for weaning food and other industrial applications. As a result, these rice bran proteins showed higher hydrophobicity than that of other rice bran protein varieties as well as more functionality. Thus, they have good potential in the food and pharmaceutical industries.