猪结合珠蛋白基因的克隆、原核表达及单克隆抗体制备

本研究的目的在于利用重组猪结合珠蛋白(Haptoglobin,Hp)制备特异性单克隆抗体.以梅山猪肝脏总RNA为模板,利用RT-PCR方法扩增猪hp基因,将其克隆到原核表达栽体pGEX-KG和pET-32a上,转化至E.coli BL21中,IPTG诱导下高效表达重组GST-Hp和HIS-Hp蛋白.用纯化的重组GST-Hp免疫BALB/c小鼠,用纯化的HIS-Hp作为包被抗原进行间接ELISA检测,通过淋巴细胞杂交瘤技术筛选阳性细胞株,制备单克隆抗体.结果表明筛选到2株高效分泌抗猪Hp单克隆抗体的杂交瘤细胞株,命名为3B4和3C8.Western-blot和间接ELISA检测均表明制备的单克隆抗体具有良好的特异性.     

城市空间增长效应评价方法——以重庆市为例

在城市空间快速增长背景下,开展城市空间增长效应评价研究,有助于解决城市空间增长中出现的问题,可为确定城市空间发展战略、制定空间发展政策提供依据。城市空间增长效应评价方法旨在定量、客观地判断城市空间增长过程中要素配置与运行是否合理、高效和公平。笔者建立了结构配置效应、经济配置效应和公平配置效应3个方面的评价体系,通过目标法对城市空间增长效应进行评价,得出空间增长效应评价函数。以重庆市为例进行实证分析,结果显示：重庆市2004-2013年间的城市空间增长效应总体呈上升发展趋势,结构性、公平性趋好;合理配置效应和经济配置效应波动显著,反映出城市建设面临的用地结构优化、用地效率提升等方面的问题。     

有益菌根真菌及其互作对带叶兜兰试管苗生理生长的影响

为探讨菌根真菌的互作效应,筛选协同互作模式组合,对从野生兜兰新鲜营养根中分离、筛选出的3种菌根真菌（PF02、PF06和PF07）,采用单菌接种、双菌混合接种和三菌混合菌种等7种接种方式,开展菌−苗共生培养,筛选出优势菌株PF02和PF07,揭示在实验室环境下接菌方式与接种效应之间的相互联系,分析菌根真菌互作对带叶兜兰植株生长和生理的影响。结果表明：显示菌根真菌互作对宿主植株的生物量、保护酶活性及叶绿素总量具有较好的正向效应。3种双菌混合接种方式PF02−PF06、PF02−PF07及PF06−PF07均能有效促进带叶兜兰生物量增长,尤其是PF02−PF06接种方式,抗氧化酶POD、超氧化物歧化酶SOD、过氧化氢酶CAT活性及叶绿素总量提高显著。选择优化的接种方式,以及能够协同促进、达到共生平衡的最佳接种方式组合,最大限度发挥菌根对宿主植物的益生作用。     

Effect of Steeping Conditions on Wet-Milling Characteristics of Hard Red Winter Wheat

A batch-wise small-scale wet-processing laboratory for whole wheat kernel has been designed and constructed to produce wheat starch and gluten from wheat grains. Hard red winter wheat kernels were steeped in three steeping media: SO₂ solution, lactic acid, and hydrochloric acid. Acid concentrations of 0.1, 0.3, and 0.5%, were used for SO₂ solutions and hydrochloric acid, and 0.1, 0.6, and 3.0% for lactic acid. After 16, 20, and 24 hr of steeping, the wheat was wet-milled. Yields and protein contents of wet-milling fractions were compared. Both high concentration of steeping media and long steeping time increased the starch yield and decreased the protein contents of the starch. However, the steeping time and acid concentration could be reduced from 24 to 20 hr and from 0.5 to 0.3%, respectively, without any statistically significant difference in starch yields or protein contents of the starch. Consistency and color of the starch were affected by both steeping time and acid concentrations of steeping media.     

Forest biomass and root–shoot allocation in northeast China

Temperate and boreal forests act as major sinks for atmospheric CO₂. To assess the magnitude and distribution of the sinks more precisely, an accurate estimation of forest biomass is required. However, the determinants of large-scale biomass pattern (especially root biomass) are still poorly understood for these forests in China. In this study, we used 515 field measurements of biomass across the northeast part of China, to examine factors affecting large-scale biomass pattern and root–shoot biomass allocation. Our results showed that, Picea & Abies forest and coniferous & broadleaf mixed forest had the highest mean biomass (178–202 Mg/ha), while Pinus sylvestris forest the lowest (78 Mg/ha). The root:shoot (R/S) biomass ratio ranged between 0.09 and 0.67 in northeast China, with an average of 0.27. Forest origin (primary/secondary/planted forest) explained 31–37% of variation in biomass (total, shoot and root), while climate explained only 8–15%, reflecting the strong effect of disturbance on forest biomass. Compared with shoot biomass, root biomass was less limited by precipitation as a result of biomass allocation change. R/S ratio was negatively related to water availability, shoot biomass, stand age, height and volume, suggesting significant effects of climate and ontogeny on biomass allocation. Root–shoot biomass relationships also differed significantly between natural and planted forests, and between broadleaf and coniferous forests. Shoot biomass, climate and forest origin were the most important predictors for root biomass, and together explained 83% of the variation. This model provided a better way for estimating root biomass than the R/S ratio method, which predicted root biomass with a R² of 0.71.     

大豆茎生长习性分类方法的研究--有限型与无限型茎顶的特征与识别标记

大豆有限型与无限型茎生长习性主要区别在于：前茎顶有顶生总状花序（或花序轴、或英簇）、三裂苞片、小苞片；后茎顶有复叶及其托叶（2个，生于复叶基部两侧）。针对大豆有限型和无限型茎顶性状发育的不同特点，提出稳定的识别标记及适用时期。     

品种及初生重对仔猪死亡率和21日龄个体重的影响

试验对2 319头仔猪的初生重与21日龄个体重和死亡率进行分析。结果表明:大约克仔猪哺乳期死亡率最低,21日龄体重最高;随着初生重的增加,仔猪死亡率降低,21日龄个体重增加。     

裂叶红麻的光合器官数量性状与麻皮产量相关性分析

对６个裂叶红麻品种（系）不同光合器官的数量性状与干皮产量作了相关分析。结果表明总叶数、７裂叶数及麻皮长和麻皮宽的光合器官之间及与干皮产量之间的相关都是显著的，而圆叶数、３裂叶数及５裂叶数与干皮产量相关不显著。总对数、７裂叶数及麻皮长的数量性状其广义和狭义遗传力都很低．表明主要光合器官的表现易受环境影响，注意选择圆叶数性状可能有助于培育高产品种。