L-carnitine significantly decreased aging of rat adipose tissue-derived mesenchymal stem cells

Mesenchymal stem cells are undifferentiated cells that have the ability to divide continuously and tissue regeneration potential during the transplantation. Aging and loss of cell survival, is one of the main problems in cell therapy. Since the production of free radicals in the aging process is effective, the use of antioxidant compounds can help in scavenging free radicals and prevent the aging of cells. The aim of this study is evaluate the effects of L-carnitine (LC) on proliferation and aging of rat adipose tissue-derived mesenchymal stem cells (rADSC). rADSCs were isolated from inguinal region of 5 male Rattus rats. Oil red-O, alizarin red-S and toluidine blue staining were performed to evaluate the adipogenic, osteogenic and chondrogenic differentiation of rADSCs, respectively. Flow cytometric analysis was done for investigating the cell surface markers. The methyl thiazol tetrazolium (MTT) method was used to determine the cell proliferation of rADSCs following exposure to different concentrations of LC. rADSCs aging was evaluated by beta-galactosidase staining. The results showed significant proliferation of rADSCs 48 h after treatment with concentrations of 0.2 mM LC. In addition, in the presence of 0.2 mM LC, rADSCs appeared to be growing faster than control group and 0.2 mM LC supplementation could significantly decrease the population doubling time and aging of rADSCs. It seems that LC would be a good antioxidant to improve lifespan of rADSCs due to the decrease in aging.     

Vasovagal tonus index (VVTI) as an indirect assessment of remission status in canine multicentric lymphoma undergoing multi-drug chemotherapy

Vasovagal tonus index (VVTI) is an indirect measure of heart rate variability and may serve as a marker of disease severity. Higher heart rate variability has predicted lower tumour burden and improved survival in humans with various tumour types. The purpose of this pilot study was to evaluate VVTI as a biomarker of remission status in canine lymphoma. The primary hypothesis was that VVTI would be increased in dogs in remission compared to dogs out of remission. Twenty-seven dogs were prospectively enrolled if they had a diagnosis of intermediate to high-grade lymphoma and underwent multidrug chemotherapy. Serial electrocardiogram data were collected under standard conditions and relationships between VVTI, remission status and other clinical variables were evaluated. VVTI from dogs in remission (partial or complete) did not differ from dogs with fulminant lymphoma (naive or at time of relapse). Dogs in partial remission had higher VVTI than dogs in complete remission (p = 0.021). Higher baseline VVTI was associated with higher subsequent scores (p < 0.001). VVTI also correlated with anxiety level (p = 0.03). Based on this pilot study, VVTI did not hold any obvious promise as a useful clinical biomarker of remission status. Further investigation may better elucidate the clinical and prognostic utility of VVTI in dogs with lymphoma.     

女民警带犬训练中的几点注意

随着我国警犬技术工作的发展,警犬技术的应用领域越来越广,从业人员也越来越多,此前只有男民警从事的职业也逐渐涌现出一批女民警,使得带犬女民警在北京、上海、合肥、郑州等城市的应用出现了一道亮丽的风景线。女民警带犬在给警犬技术带来活力的同时,其性别优势在警犬技术的训     

Integrated approach for improving small scale market oriented dairy systems in Pakistan: participatory rural appraisal and economic opportunity survey

Livestock production is an integral part of the rain-fed and irrigated agriculture system in Pakistan. Animal production is closely interlinked with the cropping systems and play a crucial role in the rural economy. Participatory rural appraisals and economic opportunity surveys were conducted in two ecological zones (irrigated and rain-fed) and two dairy production systems (peri-urban and mixed livestock). Major constraints in animal health, nutrition and reproduction were identified and interventions were suggested to overcome these difficulties. The economic opportunity survey revealed that maximum opportunity to enhance farmers’ income is to increase milk production per day per animal, which can be accomplished through coordinated improvements in nutrition, reproduction and genetics.     

俄罗斯引进鲁梅克斯新品种在黑龙江省的推广种植

黑龙江省北部全年低温时间较长,西部地区土地盐碱化严重,是制约黑龙江农牧业发展的两大主要因素。为了筛选出与黑龙江省气候和土壤类型相适应的牧草,本研究对从俄罗斯引进的鲁梅克斯新品种‘ЩавельЧемпион’在黑龙江省不同积温带的5个具有代表性的种植点阿城、肇州、逊克、嫩江和呼玛的农艺性状和营养价值进行研究。结果表明,该品种在肇州的生长状况明显差于其它地区,而在嫩江的生长状况明显优于其它地区;产量则表现为阿城、逊克和嫩江较高,产量均在150t·hm-2以上,肇州和呼玛略低,但产量也均在120t·hm-2以上。从营养数据可以看出,阿城种植区的粗蛋白及粗脂肪含量显著高于其它地区(P0.05),其中粗蛋白为18.41%,粗脂肪为3.82%;肇州种植区的粗纤维含量显著低于其它地区,含量为10.75%,而粗灰分含量显著高于(P0.05)其它各种植区,达到14.85%;嫩江种植区钙含量显著高于其它地区;肇州种植区的干物质含量显著高于其它地区。结果表明,引进新品种‘ЩавельЧемпион’适应性强,能够在黑龙江省不同区域不同土壤上种植并推广。     

虾蛄蛋白浆对河蟹摄食和生长影响的研究

分别用虾蛄蛋白浆替代饲料中2%和4%的鱼粉,并以乌贼膏为对照,研究其对河蟹摄食和生长的影响。结果表明,饲料中添加虾蛄蛋白浆对河蟹的诱食作用与乌贼膏相当,但摄食量显著高于乌贼膏组。此外,虾蛄蛋白浆还具有明显的促进河蟹脱壳生长的作用,其作用机制可能与其中氨基酸、小肽、胆固醇等含量丰富有关。     

新疆乌鲁木齐市犬绦虫感染状况调查

为了解乌鲁木齐市的犬绦虫感染情况,对2012—2015年在乌鲁木齐市8个区(县)采集的17 710份散养犬粪便,采用直接涂片法和漂浮法进行检查,对绦虫卵进行计数,统计感染率。结果共检出阳性样品1 870份,感染率为10.56%;半农半牧型区县的犬绦虫感染情况较主城区区县严重;4年内各区县的犬绦虫感染率整体呈下降趋势。建议各区县落实"犬犬驱虫,月月投药"策略,加强对城市流浪犬的管理。     

"双链型"草-鹅-稻生态农业模式高产高效配套技术

提出了“双链型”单-鹅-稻生态农业模式，总结了稻田套种牧草的方法和田间管理技术，探讨了种草与养鹅在时间上的相互衔接，以提高牧草的产量和利用率，进行了稻田套种牧草养鹅与种植小麦的比较效益分析。该配套技术的实施，为当前开展农业结构调整开辟了一条新路。     

Effect of supplementation of mustard oil cake on intake,digestibility and microbial protein synthesis of cattle in a straw-based diet in Bangladesh

The objective of this study was to analyse the effects of different levels of rumen-degradable protein (RDP) on intake, digestibility and microbial protein synthesis by supplementing mustard oil cake (MOC) on rice straw-based diet of cattle (Bos indicus) in Bangladesh. A 4 × 4 Latin square design was applied. Four diets having constant energy (7.0 MJ/kg of dry matter (DM)) with varying levels of RDP (M ₀ = 4.1 g/MJ (control), M ₁ = 6.3 g/MJ, M ₂ = 8.3 g/MJ and M ₃ = 12.4 g/MJ of metabolizable energy (ME)) were received by each animal for a period of 28 days. A metabolism trial was conducted for 7 days. Results indicate that with increasing levels of RDP, crude protein (CP) and RDP intake increased significantly (P < 0.01). The significant (P < 0.01) increase in digestibility values are obtained for DM, organic matter, CP and digestible organic matter in the rumen. The digestibility of neutral detergent fibre and acid detergent fibre was also increased significantly (P < 0.05). The total nitrogen (N), ammonia-N and total volatile fatty acids increase significantly (P < 0.01) while the rumen pH increased from M ₀ to M ₂ and decreased thereafter. The efficiency microbial N intake increased significantly (P < 0.01) but showed a curvilinear response with higher RDP level (12.40 g/RDP/MJ ME). This study concludes that supplementation of RDP from MOC enhances the intake, digestibility and microbial protein synthesis which ultimately increases utilization of low-quality feed resources that can be used for developing cost-effective feeding systems on a straw-based diet in tropical regions.