Effects of puerarin on apelin-12, angiotensin II,NO and blood pressure in renovascular hypertensive rat

AIM: To investigate the effects of puerarin on blood pressure in renovascular hypertensive rats, and to measure puerarin-induced changes of apelin-12, angiotensin II (Ang II) and nitric oxide(NO), the factors related to development of hypertension. METHODS: Sixty-five male Sprague-Dawley rats were used, of which 8 rats were randomly selected as sham operation group, and the remaining were used to make two-kidney, one-clip model. The rats that met the criterion for Goldblatt hypertensive rat model were randomly allocated into 5 groups: high-, middle- and low-dose puerarin groups, captopril group, and model group. The drugs were administered for 6 weeks. Blood pressure was measured every 2 weeks. Six weeks after treatment, all rats were sacrificed under deep anesthesia. Blood and kidney samples were collected. The level of apelin-12 in serum and kidneys was detected by ELISA. The level of Ang II in plasma and kidneys was measured by radioimmunoassay. NO level in serum was examined by nitrate reductase assay. RESULTS: Puerarin had an antihypertensive effect in a dose-dependent manner. Marked decreases in the level of serum apelin-12 in high- and middle-dose puerarin groups were observed(P<0.01). Puerarin at low dose did not cause obvious change in the content of apelin but still reached significant level (P<0.05). As the dose of puerarin went up, the level of apelin-12 in the kidneys was gradually decreased. Puerarin at high and middle doses obviously reduced the level of AngII in plasma, while purarin at low dose did not produce any significant effects. Puerarin at high and middle doses markedly increased the level of NO in serum, but puerarin at low dose did not induce any significant changes. CONCLUSION: Puerarin has an antihypertensive effect, and its mechanism may be related to inducing the changes of apelin, Ang II and NO, and regulating the balance among those factors.     

兰花根外敷治疗韧带扭伤308例

目的对湖北京山县大洪山的野生蕙兰(Cymbidium faberi Rolfe)植物群落进行调查,以期为蕙兰的保护提供科学支撑。方法通过样方法调查测量样地中蕙兰的地理坐标、海拔、坡向、坡度、郁闭度、盖度、土壤类型和群落物种组成。结果蕙兰群落物种丰富,44种维管束植物分属31科39属,其中高位芽植物种类最多,地下芽植物次之,层间有丰富的藤本和附生植物。乔木层多见柏科、壳斗科、棕榈科和松科,灌木层蔷薇科、樟科和紫金牛科较为多见;蕙兰为群落下层优势种,区系特征涉及热带、亚热带、温带分布型,有北亚热带向南暖温带过渡的明显趋势。结论蕙兰适合生长在水热充足、林间有散射光、郁闭度适宜、通风条件好、有较厚落叶层的酸性土壤中。     

Can sieving size affect the determination of soil available micronutrients?

<正>Dear Editor,Globally, approximately two billion people suffer from micronutrient deficiencies(Tulchinsky, 2010; Myers et al.,2014).Soil micronutrient availability is of great importance for the evaluation of soil fertility and the determination of appropriate measures for improving crop quality and human health.The diethylenetriamine-penta-acetic(DTPA)     

急性感染猪瘟病毒猪体外排毒规律的观察

为研究CSFV感染后在体外的传播途径、排毒规律,针对CSFV基因组设计了一对引物和一条探针,建立了一套CSFV荧光定量PCR(FQ-PCR)检测方法,并以质粒为标准品得到扩增标准曲线.对18个CSFV阳性质控样本检测全阳性,6个CSFV阴性质控样本检测全阴性,显示良好敏感性和特异性.应用此方法对石门株感染的16头60日龄长白猪和1头阴性对照猪的粪便、尿液、眼分泌物和唾液中病毒含量进行了动态测定,结果表明从感染后第1天到频死前第8天,粪便中均能检测出病毒;尿液和眼分泌物至少能从第3天,唾液从第4天开始检测出病毒,且病毒含量呈增加趋势.本研究对急性感染猪瘟病毒猪体外排毒规律进行了系统研究,为弄清CSFV感染病程、致病机理及临床诊断奠定了重要理论基础.     

不同品种与种植密度对旱地玉米产量及水分利用效率的影响

在干旱条件下,选择农大108和郑单958为供试品种,研究其在不同种植密度下的群体产量、产量构成因素及水分利用效率的差异。结果表明,相同密度下,郑单958产量高于农大108;农大108在低密度下,穗粒数占有优势;在高密度下,郑单958在穗粒数和百粒质量上占优势;在水分利用效率方面,不同种植密度下郑单958均高于农大108。     

中国南方水乡乡村聚落空间结构研究综述

为了解水乡乡村聚落空间研究进展,运用文献统计分析和内容分析法,总结1915—2021年中国南方水乡乡村聚落空间结构特征、演变过程及影响因素的研究进展.结果表明:区域层面主要基于地理学、景观生态学和城乡规划学进行定量研究,村域层面主要基于建筑学、城乡规划学和风景园林学进行定性研究;不同时期的研究存在内容差异和方法差异,不...     

福建省油茶林主要传粉昆虫种类及访花行为

对福建省福州、泉州、龙岩、南平、宁德等地油茶林传粉昆虫及其访花行为进行了调查,结果表明:福建省油茶林中主要传粉昆虫有24种,分属于3目11科17属,其中膜翅目的昆虫种类最多,有11种;大分舌蜂是油茶最重要的传粉昆虫,其数量多,传粉效率高;传粉昆虫日活动时间在12∶00—14∶30出现高峰,昆虫个体数量平均占全天总数的70%左右;油茶传粉昆虫的访花行为因昆虫的访花目的不同而有较大差异,温度和光照是影响传粉昆虫数量、行为和频率的主要因素。     

不同栽培环境条件对优质杂交稻培杂双七稻米品质的影响研究

通过在不同地区、不同季节进行不同插植密度和施肥条件的大田种植试验,对培杂双七稻米品质的变化进行了研究.结果表明:华南稻区早晚季种植、珠江三角洲地区和丘陵地区种植以及不同插植密度和混施硅肥、沸石均对部分品质性状产生显著的影响,但是所有这些环境条件中没有一种能明显改善该组合的全部品质性状或稻米的综合品质.因此,杂交稻的综合品质改良,只有通过不育系和恢复系的遗传改良才能从根本上解决问题.     

中国鱿鱼钓机装备研究现状及展望

鱿鱼钓机是远洋鱿钓渔业的重要生产工具。目前鱿鱼钓机装备发展面临的主要问题是机钓产量低。本文介绍了我国鱿鱼钓机装备的研究现状,对钓机运转速度、作业水深控制、抖动模式设置及结构设计等方面的研究趋势进行了探讨。最后,提出了我国鱿鱼钓机发展对策,研究指出需重点解决钓机结构优化设计、控制系统性能优化、运行参数优化等问题,为远洋鱿钓渔业的可持续发展提供参考依据。     

圆叶决明草粉替代不同比例精料喂兔效果研究

利用豆科牧草圆叶决明Chamaecrista routundifolia CPI34721草粉分别以10%,15%,20%不同比例替代精料进行肉兔喂养试验.试验结果表明:与对照相比较,在兔只平均日增重、饲料利用率方面,各处理与对照差异不显著.替代10%和15%精料处理可显著提高兔肉品质,替代20%精料的处理在经济效益方面最佳,可提高经济效益5.15%.