基于可控性生态减渗目标的河床底质重构技术研究

位于地下水超采区且具有景观要求的河流,需同时具备维持生态水位和渗透补给地下水的功能,研究可调控减渗效果的河床底质重构技术对维持河床垂向连通性具有重要意义。以华北平原常见河床底质和膨润土为主要材料配比得到重构底质,通过变水头渗透试验与模型入渗试验探究了重构底质减渗特性,根据不同河流条件提出不同的配比及结构方案,减渗层厚度和纳基膨润土配比方案1为100 mm和9%,方案2为150 mm和15%,方案3为200 mm和18%;利用GeoStudio SEEP/W软件计算模拟了不同方案的渗流及渗流量减少状况。结果表明,改变膨润土类型和掺量可调节底质的减渗效果,使其渗透系数达到小于1×10^-7cm/s和小于1×10^-6cm/s目标水平;纳基膨润土掺量为15%的重构底质在1 m水头渗透作用下,浸润锋经39 d运移15 cm,56 d内累计入渗量为11.1 cm,减渗效果较好;模拟结果表明,3种减渗方案有不同的减渗效果,典型断面河流水位在0.3～2.0 m时,3种方案河流渗流损失量可降低55.8%～68.8%、91.5%～94.3%和94.8%～96.5%...     

大豆脂肪氧合酶LOX-1热失活的高级结构变化分析

为促进大豆脂肪氧合酶LOX-1的动力学及催化机理研究,并为LOX-1活性的合理调控提供科学依据,本研究利用FT-IR技术分析LOX-1在20～80 ℃范围内的热失活和高级结构变化,采用Omnic 6.0和Peakfit 4.12软件处理FT-IR光谱,结合傅里叶自去卷积(FSD)、二阶导数分辨和曲线拟合技术分析酰胺Ⅰ带...     

沈阳地区不同利用类型耕地土壤肥力现状评价

采集沈阳地区不同利用类型耕地土壤样品425个,对土壤养分指标进行测试分析。结果表明：沈阳地区土壤有机质、有效磷和速效钾整体上属于丰富水平,全氮属于很丰富水平,土壤有效硅、交换性钙、交换性镁平均含量大于临界值,有效铜、有效锌、有效铁、有效锰属于很丰富水平,有效硼属于中等水平,总体肥力较高。     

厚层客土喷播基质中不同有机质添加物对护坡植物早期生长的影响

针对目前大量开挖草炭土用于厚层客土喷播绿化工程,导致严重破坏草炭富集区的植被,造成大量新裸露地的现状．本研究将3种不同的农业废弃物组合：Ⅱ、生玉米秸秆＋生活垃圾堆肥,Ⅲ、生玉米秸秆＋尿素堆肥,Ⅳ、生玉米秸秆来替代草炭土应用于厚层客土喷播绿化工程中,与草炭土（I）的绿化防护进行对比试验,对其理化性质及植物初期生长状况进行对比分析．结果表明,对照样方混合基质各项指标均满足植物正常生长所需的条件,植物早期生长状况良好,能达到有效代替草炭土的效果,具有较高的可行性．     

牛体细胞核移植方法研究

研究了牛体细胞核移植过程的去核方法、融合条件等对重构胚发育的影响,旨在探索一种简化的核移植操作程序.结果表明,添加FSH,LH,最高成熟率为81.7%.在电压为2.6 kV/cm时,融合率为93.96%.激活后重构胚在压制的WOW内进行培养,采用半卵法去核的卵裂率和8-细胞发育率分别为61.2%和3.27%,盲切法去核的卵裂率和8-细胞发育率分别是 70.8%和7.56%.     

Factors influencing seed reserve utilization during seedling establishment in maize inbred lines

Strong seedlings are essential for high yield. To explore the foundation of strong seedlings, we investigated various factors influencing the conversion and distribution of seed storage reserves during seedling establishment in maize inbred lines. Three maize inbred lines were used to explore the effects of seed size, seed vigor, illumination duration, temperature, water content, and salt concentration of the seedling medium on the utilization of seed storage reserves during seedling establishme...     

28%多·井悬浮剂防治水稻纹枯病田间药效评价

为明确28%多.井悬浮剂对水稻纹枯病的防治效果,进行田间药效试验。结果表明：28%多.井悬浮剂对水稻纹枯病有显著的防治效果,按500g/hm^2用量,第三次药后14d对纹枯病的防效为86.13%,与常用药剂5%井冈霉素水剂3.75L/hm^2的防效相当,而且对水稻安全,值得推广应用。     

三组混配农药防治桑蓟马、桑粉虱效果试验

为筛选高效、低毒、低残留农药混配,进行三组混配农药防治桑蓟马、桑粉虱效果试验。结果表明,混配农药20%佳禾定1000倍＋10%大功臣10g/666.7m^2、80%敌敌畏1000倍＋10%大功臣10g/666.7m^2、5%锐劲特1000倍＋10%大功臣10g/666.7m^2对桑蓟马、桑粉虱具有较好的防效,药后1d防治效果为80%以上,药后3d防治效果达98%以上。     

巨尾桉苗缺铁黄化病的防治

巨尾桉苗黄化病是一种生理病害,表现为缺素症,主要是缺铁。在缺铁、排水不良、碱性的土壤容易发病,改变土壤理化性的同时施铁元素以及氮、磷、钾等大量元素能使桉苗更好地恢复生长。     

Effects of psychological stress on in vitro expression of activated surface molecules on T cells of peripheral blood from healthy persons

AIM: To study cellular and molecular mechanism involved in increasing susceptibility of infection in psychological stress persons. METHODS: Comparative studies were performed with double staining and flow cytometry analysis on immunophenotyping and in vitro expression of early activating surface molecule CD69 in response to mitogens on T cells from peripheral blood of 20 healthy college student volunteers before and after psychological stress. A series of term final examinations was defined as psychological stress. RESULTS: Immunophenotyping analysis showed no statistically significant difference in the percentage of CD2, CD3, CD4, CD8, CD19, CD20, CD16 and CD56 positive lymphocyte populations before and after psychological stress. There was a statistically significant decrease in the in vitro expression of CD69 in response to polyclonal stimulators on the T cells from persons after psychological stress than those before psychological stress. The percentage of CD69 expression (CD69⁺CD3⁺/CD3⁺%) in response to PHA and PDB in the whole blood culture for 72 hours decreased respectively from 28.1±4.1 and 80.7±6.8 on the T cells obtained before psychological stress to 17.6±3.8 and 65.8±7.9 on those obtained after psychological stress, while there was no statistically significant difference between the CD69 expression rates without stimulators on the T cells obtained before and after psychological stress. CONCLUSIONS: The effects of psychological stress to immune system is not on the level of changing proportions of the sub-populations within peripheral blood lymphocytes. Psychological stress can decrease the activating response of T cells in healthy persons, which may be responsible for the increase of susceptibility to infection in the psychological stress persons.