裂区设计下分析影响欧洲菊苣产量的因素

在裂区设计下,分析影响欧洲菊苣（Cichorium intybus cv.Europ）产量的7个因素,结果表明：播种季节对株高、产量有显著性影响;播种方式对株高、茎粗、密度、盖度、产量5个指标均有显著影响。进一步通过因子分析得出欧洲菊苣产量最佳组合为撒播、密度在50～59株·m^-2、盖度为94%～100%。     

Research on the Antioxident Function of Sulforaphane on the Leydig Cells of Cadmium Exposured Mice

The study was aimed to explore the antioxident function of sulforaphane (SFN) on the leydig cells of cadmium (Cd) exposured mice.Cd and SFN were added to the cell culture medium of TM3 cell, half inhibitory concentration (IC₅₀) of Cd and SFN safe dose range were determined.The tested model was set up,and the relative survival rate of TM3 cells, lactate dehydrogenase (LDH) activity and cell antioxidant levels were determined to study the antioxident function of SFN to cadmium exposured mice.The results showed that:①With the increase of Cd concentration,the relative survival rate of TM3 cells was decreased,and the IC₅₀ of Cd was 51.4 μmol/L;Within a certain concentration range,SFN could increased the cells survive rate,but there was toxicity when the SFN concentration was more than the scope,and the greater the concentration,the greater the toxicity. At experimental condition,SFN safety concentration were 2.5,5 and 10 μmol/L.②Compared with the control group,the GSH content,the T-SOD and GSH-Px activities in Cd group were significantly or extremely significantly decreased (P < 0.05;P < 0.01),and the LDH activity,the MDA content were increased.Additionally,the LDH activity and MDA content of SFN groups were decreased,while others three indexes were increased. Compared with Cd group,the GSH content,the T-SOD and GSH-Px activities of Cd+SFN groups were significantly or extremely significantly increased (P < 0.05;P < 0.01),however,the LDH activity,MDA content were decreased. The result indicated that SFN had the antagonism effect on toxicity of Cd in TM3 cell.     

四种植物提取物对斜纹夜蛾卵的毒杀效果

以斜纹夜蛾卵为研究对象．对雷公藤Tripter gium wilfordii茎、皂荚Gleditsia sinensis Lam．刺、苦参Sophora flavescens和博落茴Macleya cordata等4种植物材料的正已烷及乙酸乙酯提取物的杀卵活性进行了测定。结果表明：博落茴的乙酸乙酯提取物对斜纹夜蛾卵有较好的杀伤力,校正未孵化率高达95．63%;雷公藤的乙酸乙酯提取物和苦参的正已烷提取物对斜纹夜蛾卵的孵化也有明显的抑制作用。     

达乌尔黄鼠染色体组型和G带分析

本文研究了达乌尔黄鼠东北亚种的骨髓细胞染色体组型和Ｇ－带带型。确定其染色体数为２ｎ＝３６，各染色体有其特有Ｇ－带带型。     

论生物多样性与建设海南国际旅游岛的关系

经过多年的研究和实践,人们已深刻地认识到,海南省的发展战略应该积极发展服务型、开放型和生态型经济,形成以旅游业为龙头、以现代服务业为主导的特色经济结构。显然要实现这个宏伟的目标,生物多样性的保护和持续利用就是最根本和最关键的问题。如果作为生态旅游基本资源的季节性雨林、红树林、海洋生物资源和农业生物多样性被毁,一切都将是一纸空文。本文就拟简略地探讨一下有关这些方面的问题,并提出一些建议供有关方面参考。     

A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci-infected domestic poultry in Zhejiang Province,China

A cluster of Chlamydia psittaci (C. psittaci) cases was reported in Zhejiang Province, China, 2019. This study evaluates the extent of the outbreak and determines the source of infection. Real-time PCR and sequencing of the ompA gene of C. psittaci were performed to identify the cases, the domesticated poultry and close contacts. The index patient was a 76-year-old woman with chronic vertigo, and Case 2 was a 64-year-old female farmer with herpes zoster. Both women bought psittaci-infected chickens or ducks from the same mobile street vendor and raised them for 10 days and 23 days before fever onset. There were no direct contact between the two women. C. psittaci test was positive for the two patients, one sick chicken, three healthy ducks and the vendor's chicken cage. Phylogenetic analysis showed that all seven C. psittaci positive samples carried identical ompA genotype A of C. psittaci. Of all of the patients' 148 close contacts, none tested positive for C. psittaci, or developed acute respiratory symptoms. Both patients were discharged after a 4-week hospital stay. In conclusion, the source of this cluster was the poultry infected with C. psittaci, which occasionally cause infections in farmers, but inter-human transmission seems unlikely.     

Pharmacokinetics and bioavailability of valnemulin in broiler chickens

Wang, R., Yuan, L.G., He, L.M., Zhu, L.X., Luo, X.Y., Zhang, C.Y., Yu, J.J., Fang, B.H., Liu, Y.H. Pharmacokinetics and bioavailability of valnemulin in broiler chickens. J. vet. Pharmacol. Therap. 34 , 247–251. The objective of this study was to investigate the pharmacokinetics and bioavailability of valnemulin in broiler chickens after intravenous (i.v.), intramuscular (i.m.) and oral administrations of 10 mg/kg body weight (bw). Plasma samples were analyzed by high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS). Pharmacokinetic characterization was performed by non‐compartmental analysis using WinNonlin program. After intravenous administration, distribution was wide with the volume of distribution based on terminal phase(V_z) of 4.27 ± 0.99 L /kg. Mean valnemulin t_1/2β(h), Cl_β(L /h /kg), V_ss (L /kg) and AUC_(0–∞)(μg·h /mL) values were 2.85, 0.99, 2.72 and 10.34, respectively. After intramuscular administration, valnemulin was rapidly absorbed with a C_max of 2.2 μg/mL achieved at 0.43 h (t_max), and the absolute bioavailability (F) was 88.81%; and for the oral route the same parameters were 0.66 ± 0.15 μg/mL, 1.54 ± 0.27 h and 74.42%. A multiple‐peak phenomenon was present after oral administration. The plasma profile of valnemulin exhibited a secondary peak during 2–6 h and a tertiary peak at 32 h. The favorable PK behavior, such as the wide distribution, slow elimination and acceptable bioavailability indicated that it is likely to be effective in chickens.     

日本曲霉和顶青霉的毒性试验

对从云南省盘溪和剑川地区发霉稻草中分离出的日本曲霉(Aspergillus japonicusSaito),从盘溪发霉稻草和剑川马匹粪便中分离出的顶青霉(Penicillium corylophilum Dierckx)分别进行了荧光检测、脂肪分解2 项理化学特性鉴定。将2 株菌的固体和液体培养物,置室温产毒再经杀菌等方法处理后,进行了家兔皮肤涂抹试验,雏鸭和家兔的菌粮饲喂试验,小鼠皮下、腹腔注射试验,家兔耳缘静脉注射试验。日本曲霉能产生荧光,分解脂肪,使家兔皮肤增厚和引起红肿、结痂,能引起部分试验雏鸭、全部试验小鼠、部分试验家兔死亡;顶青霉能产生荧光,使家兔皮肤增厚和出现轻微红肿反应,能引起全部试验小鼠死亡。剖检试验动物均见程度不同的病理学改变。以上结果表明,日本曲霉和顶青霉对小动物均具有一定的毒力。