不同培养基成分对薄荷试管苗生根的影响

[目的]筛选薄荷试管苗生根的最佳培养基组合。[方法]以薄荷试管苗茎段为试验材料,探讨不同培养基成分、凝固剂种类及添加量对其生根的影响。[结果]在薄荷试管苗培养过程中,使用琼脂为凝固剂,且添加量为7 g/L的1/2MS培养基时,其生根时间最短,生根数和生根长度较高。[结论]为薄荷试管苗的大规模、优质、高效生产提供理论依据。     

油松专用肥对苗木生理特性及抗性的影响

油松是华北地区重要的造林树种,合理施肥可有效提高油松体内的养分和抗性。在4种不同施肥处理下,对3年生油松幼苗的叶绿素、根系活力、电导率的动态变化进行了测定,以探讨施肥对苗木生理特性及抗性的影响。结果表明,施肥对油松各种生理特性有明显影响;除树液流动期外,不同施肥处理能显著提高叶绿素含量,且效应由大到小排序为多元肥NPK肥N肥;施肥能显著提高根系活力,施用多元肥对根系活力呈现极显著差异的正效应;不同施肥处理对油松电导率的影响程度没有明显的规律。     

Analysis of the independent-and interactive-photo-thermal effects on soybean flowering

Soybean(Glycine max(L.) Merr.) is a typical short-day and warm season plant, and the interval between emergence and flowering has long been known to be regulated by environmental factors, primarily photoperiod and temperature. While the effects of photoperiod and temperature on soybean flowering have been extensively studied, a dissection of the component photo-thermal effects has not been documented for Chinese germplasm. Our objective of the current study was to evaluate the independent- and interactive-photo-thermal responses of 71 cultivars from 6 ecotypes spanning the soybean production regions in China. These cultivars were subjected in pot experiments to different temperature regimes by planting in spring(low temperature(LT)) and summer(high temperature(HT)), and integrating with short day(SD, 12 h), natural day(ND, variable day-length), and long day(LD, 16 h) treatments over two years. The duration of the vegetative phase from emergence to first bloom(R1) was recorded, and the photo-thermal response was calculated. The outcome of this characterization led to the following conclusions:(1) There were significant differences in photo-thermal response among the different ecotypes. High-latitude ecotypes were less sensitive to the independent- and interactive-photo-thermal effects than low-latitude ecotypes; and(2) there was an interaction between photoperiod and temperature, with the effect of photoperiod on thermal sensitivity being greater under the LD than the SD condition, and with the effect of temperature on photoperiodic sensitivity being greater under the LT than the HT condition. The strengths and limitations of this study are discussed in terms of implications for current knowledge and future research directions. The study provides better understanding of photo-thermal effects on flowering in soybean genotypes from different ecotypes throughout China and of the implications for their adaptation more broadly.     

Establishment and Optimization of INH Epitope Peptide Vaccine for Detection of Antibody

In this study, an indirect ELISA method was established to detect inhibin hormone (INH) epitope peptide vaccine antibody, it would provide oretical reference for the determination of Fine-wool sheep after active immune body INH epitope peptide vaccine antibody. On the basis of the predecessors, using indirect ELISA method to determinate serum INH epitope peptide antibody levels of sheep, and through control different experimental conditions to look for the best experimental conditions. Through explorating the experimental conditions, finally, the testing experiment conditions were determined, which was blocked solution with skimmed milk powder, INH and GnIH synthetic peptides dilution degrees for 20 000 times, the optimum reaction time was 60 min, the best color action time was 15 min. In this experiment, a kind of method to detection antibody in the body after INH active immune sheep was built, it would provide a reference for future research.     

不同药剂组合对马铃薯晚疫病的防治效果

马铃薯晚疫病是马铃薯生产中的重要病害,是影响马铃薯产量和品质的重要因素之一,在云南春作马铃薯上普遍发生,且危害较重,给当地马铃薯产业带来了巨大的经济损失。为减轻马铃薯晚疫病对马铃薯生产造成的损失,试验研究了11种药剂7种药剂组合(包衣剂+保护剂+治疗剂)通过播种期种薯包衣,现蕾期叶面喷施一次保护剂,发病初期和发病中期叶面各喷一次治疗剂的方法防治马铃薯晚疫病。结果表明,药剂组合种薯包衣+甲霜·锰锌+氟吡菌胺·霜霉威的防治效果最好,防治效果达到60.99%,增产率176.28%,增效率152.27%;其次是种薯包衣+霜脲·锰锌+氟吡菌胺·霜霉威组合,防治效果达到60.15%,增产率173.20%,增效率150.55%。这2个药剂组合对马铃薯晚疫病均有极显著的防治效果,增产增效明显。     

日粮补充苹果渣或有机矿对鹅生产性能、蛋品质及繁殖性能的影响

本研究旨在评估日粮单独添加苹果渣或补充苹果渣和有机微量元素对鹅生产、繁殖性能及蛋品质的影响。试验将产蛋期的360只鹅随机分为3组,每组120只(30只/重复),对照组饲喂基础日粮,处理组分别饲喂苹果渣日粮和苹果渣+有机微量元素日粮,试验持续14周。结果:苹果渣补充或不补充有机矿较对照组显著提高了孵化率和仔鹅存活率(P<0.05),苹果渣+有机矿组较其他两组显著提高了蛋长度、蛋壳尖端和钝端厚度(P<0.05),但显著降低了蛋型指数、蛋壳重量和蛋黄重量(P<0.05)。日粮添加苹果渣同时补充有机矿较对对照组显著提高了鹅蛋中甘油二酯、胆固醇、胆固醇酯的含量(P<0.05),但苹果渣补充或不补充有机矿磷脂含量较对照组显著降低了13.17%和20.08%(P<0.05)。与苹果渣组相比,日粮添加苹果渣补充有机矿非酯化脂肪酸含量显著提高了31.76%(P<0.05)。结论:日粮添加苹果渣同时补充有机铜和锌可显著改善鹅的繁殖性能、蛋品质及蛋中脂肪组分。     

‘品丽珠’葡萄不同营养系酚类物质差异分析

筛选山东蓬莱产区酿酒葡萄‘品丽珠’黄烷醇、花色素苷含量较高的优良营养系，以期为发挥其酿酒性能提供理论依据。以蓬莱产区品丽珠母本及3个芽变营养系（214、327、623）为试材，采用分光光度计法分析葡萄果皮和种子中总酚、总单宁、总花色素含量，利用高效液相色谱法分析酚酸类、黄酮醇类、黄烷醇类、花色苷单体及白藜芦醇含量。研究结果表明，黄烷醇是种子和果皮中主要的单体酚，其中儿茶素含量最高；二甲花翠素葡萄糖苷是果皮中主要的花色苷。在相同的栽培管理条件和自然条件下，品丽珠不同营养系间的酚类物质差异较明显，相同营养系不同年份间也有显著差异。其中营养系623果皮和种子的总酚、种子的单宁和黄烷醇以及果皮和种子中的酚酸类、单体花色苷含量显著高于其他营养系和母本。