Clinical, humoral and IFNgamma responses of cattle to infection with Mycoplasma mycoides var. mycoides small colony and attempts to condition the pathogenesis of the infection

Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides var. mycoides small colony (MmmSC), is one of the most important diseases of cattle in Africa. The role of innate or acquired cell mediated and humoral immunity in conferring protection against MmmSC infection has not yet been elucidated. On the other hand, the pathological lesions caused by the aetiological agent have been considered indicative of an immunopathological process. In this study ten na?ve cattle were exposed to in-contact infection with animals infected by intubation with a strain of MmmSC. Clinical signs, antibody response, IFNgamma release and pathological changes at necropsy were analysed and compared with the events following in-contact infection of an equal number of animals kept under daily treatment with cyclosporine for the entire observation period of 84 days. Cyclosporine is a suppressor of the immune response related to the T-cell system. Under the conditions of the experiment, cyclosporine appeared to condition the pathogenesis of CBPP by delaying the events that follow infection, bringing further support to the possibility that the immune response may have an impact on the disease outcome.     

Enhancement of enrofloxacin serum antibacterial activity by calcium primed broilers

The aim of this trial was to assess the effect that calcium gluconate priming of 468 broilers has on the antibacterial activity of a standard dose of enrofloxacin. Hence, a series of oral pharmacokinetic studies were carried out in four groups of broilers medicated individually through an oral cannula as follows: group A, medicated only with enrofloxacin 10mg/kg; group B, receiving immediately one after the other, calcium gluconate (200mg/kg) and enrofloxacin 10mg/kg; group C, dosed first with calcium gluconate (200mg/kg) and 1h later enrofloxacin (10mg/kg); and group D, dosed first with calcium gluconate (200mg/kg) and 2h later enrofloxacin (10mg/kg). Broilers were bled at different times after the dose of enrofloxacin and antibacterial activity, measured as concentration of enrofloxacin, was measured by an agar diffusion assay. Results revealed that group D the greatest values of maximum serum concentration (Cs(max)), area under the concentration vs. time curve (AUC) and area under the moment curve (AUMC). These values were statistically higher than the corresponding ones derived from groups A, B and C (P<0.05). Taking Cs(max) and AUC values of group A as reference baseline, an increase of 24% and 50%, respectively, was obtained in group D. Group B had the lowest Cs(max), AUC, AUMC and elimination half life (T(1/2)beta) and these values were statistically different from groups A, C and D (P<0.05). The T(1/2)beta was statistically longer in groups C and D as compared with A and B, and the former groups were also different between each other (P<0.05). These results show that if calcium gluconate is first dosed to broilers and 2h later enrofloxacin is administered (as in group D), a more pronounced antibacterial activity of enrofloxacin can be obtained. A challenge of this sequential dosing scheme in a field trial may reveal its clinical value.     

Ticks (Acari: Ixodidae) collected from animals in three western, semi-arid nature reserves in South Africa

The objective of this study was to make an inventory of the ixodid tick species infesting wild animals in three western, semi-arid nature reserves in South Africa. To this end 22 animals in the Kgalagadi Transfrontier Park, 10 in the West Coast National Park and 16 in the Karoo National Park were examined. Fourteen tick species were recovered, of which Hyalomma truncatum, Rhipicephalus exophthalmos and Rhipicephalus glabroscutatum were each present in two reserves and the remainder only in one. The distributions of two of the 14 tick species recovered, namely Rhipicephalus capensis and Rhipicephalus neumanni, are virtually confined to the western semi-arid regions of southern Africa. Hyalomma truncatum, R. capensis and R. glabroscutatum were the most numerous of the ticks recovered, and eland, Taurotragus oryx, were the most heavily infested with the former two species and gemsbok, Oryx gazella, and mountain reedbuck, Redunca fulvorufula, with R. glabroscutatum.     

Unraveling the morphological patterns of a subantarctic eelpout: a geometric morphometric approach

Phenotypic variation in organisms depends on the genotype and the environmental constraints of the habitat that they exploit. Therefore, for marine species inhabiting contrasting aquatic conditions, it is expected to find covariation between the shape and its spatial distribution. We studied the morphology of the head and cephalic sensory canals of the eelpout Austrolycus depressiceps (4.5–22.5 cm TL) across its latitudinal distribution in South Pacific (45°S–55°S). Geometric morphometric analyses show that the shape varied from individuals with larger snout and an extended suborbital canal to individuals with shorter snouts and frontally compressed suborbital canal. There was size variation across the sampled populations, but that size does not have a clear latitudinal gradient. Only 8% of the shape variation relates to this size variation (allometry), represented by a decrease in the relative size of the eye, and a depression of the posterior margin of the head. There were spatial differences in the shape of the head, but these differences were probably caused by allometric effects. Additionally, 2 of the canals of the cephalic lateralis pores and the head shape showed modularity in its development. This study shows that the morphology of marine fish with a shallow distribution varies across distances of hundreds of kilometers (i.e., phenotypic modulation).     

Dihydrooxadiazines: Octopaminergic system as a potential site of insecticidal action

Dihydrooxadiazines are structural analogs of octopamine and were compared with octopamine for their ability to compete with [³H]dihydroergocryptine ([³H]DHE) for binding sites on DHE-sensitive receptors, to stimulate adenylate cyclase activity in nervous system homogenates of Periplaneta americana L., and to modulate the action of the peptide proctolin on the oviducal muscles of Locusta migratoria L. [³H]DHE binding was inhibited by low concentrations (μM range) of octopamine, phentolamine, N-demethylchlordimeform (DCDM) and several dihydrooxadiazines. The tested dihydrooxadiazines acted as aminergic agonists in stimulating cyclic AMP production in cockroach nervous system homogenates and did not show additive effects with octopamine, whereas additivity was observed with 5-hydroxytryptamine. The relative potency of octopaminergic antagonists, including mianserin, cyproheptadine, phentolamine, and gramine, to block octopamine-mediated elevation of cyclic AMP production was similar to the rank-order potency of the same antagonists to inhibit dihydrooxadiazine-mediated elevation of cyclic AMP production. Octopamine, 2-(4-bromophenyl)-5,6-dihydro-4H-1,3,4-oxadiazine (4-Br-PDHO), and 8-Br-cyclic AMP caused increased phosphorylation of proteins that are phosphorylated by exogenously added cyclic AMP-dependent protein kinase. These results indicate that the dihydrooxadiazine-induced rise in cyclic AMP levels in homogenates of the cockroach nervous system results directly in activation of an endogenous cyclic AMP-dependent protein kinase. 4-Br-PDHO behaved similarly to octopamine in modulating the action of proctolin-induced contractions in locust oviducal muscles. These observations suggest that dihydrooxadiazines act as octopamine agonists and have an octopaminergic action in modulating the action of proctolin. Thus, it is proposed that dihydrooxadiazines exert at least part of their insecticidal and miticidal actions through interaction with the octopaminergic system.     

1-Octanol/Water Partition Coefficient (Kow) and pKa for Ionisable Pesticides Measured by apH-Metric Method

pK_a values for a wide range of commonly used ionisable pesticides, together with the log K_ow values of the most lipophilic form of each, have been measured using pH-metric techniques. Examples of acids, bases and multiprotic compounds from the major classes of herbicides, and a number of insecticides and fungicides that contain ionisable groups, are included. The pK_a and log K_ow values so obtained were generally in good agreement with values taken from the literature that were measured by other methods. The lower limit of log K_ow that could be measured by the pH-metric method lay below the -0·97 obtained for amitrole, but the method could not be applied to glyphosate for which shake-flask measurements indicated log K_ow below -3. The highest log K_ow obtained in this study was 5·12 for pentachlorophenol. The pH-metric technique offers a rapid and convenient method to determine pK_a and log K_ow for ionisable compounds, especially when utilising an automatic titration system linked to a dedicated computer.     

Antimycotic Screening of 58 Malaysian Plants against Plant Pathogens

Ethanolic extracts of 58 Malaysian plants belonging to 24 different families were screened for antifungal activity against seven plant pathogens using the filter paper disc diffusion technique. Two varieties of Piper betle, showed strong activity against all the pathogens tested (Colletotrichum capsici, Fusarium pallidoroseum, Botryodiplodia theobromae, Alternaria alternata, Penicillium citrinum, Phomopsis caricae-papayae and Aspergillus niger), with inhibition diameters significantly (P<0·01) bigger than 2·5 mg ml⁻¹ prochloraz or 10 mg ml⁻¹ clotrimazole. The minimum inhibitory concentrations of the ethanolic extracts of P. betle against these plant pathogens ranged between 0·01 mg ml⁻¹ and 1 mg ml⁻¹. Thirty-four other plants (Kucing gala, Limau batik, Bertholletia excelsa, Bixa orellana, Caesalpinia pulcherrima, Cerbera odollam (fruits and leaves), Colocasia gigantea, Curcuma domestica, Curcuma manga, Derris eliptica, Elephantopus scaber, Eleusine indica, Eugenia polyantha, Euphorbia hirta, Euphorbia tirucalli, Gardenia florida, Hedyotis auricularia, Hibiscus rosa-sinensis, Juniperus chinensis (three varieties), Lawsonia inermis, Lecythis ollaria, Mentha arvensis, Mimusops elengi, Ocimum sanctum, Phyllanthus niruri, Piper nigrum, Piperomia pellucida, Pedilanthus tithymaloides, Polygonum minus, Spondias dulcis, Solanum nigrum, Tinospora tuberculata) showed selective antifungal activity, while 21 species were inactive.