鸡霍乱口服弱毒疫苗的研究

用经过选择的禽多杀巴氏杆菌自然弱毒菌株Ｒ１－２３菌株制成的鸡霍乱固体培养口服弱毒疫苗，其安全剂量接近于２００个免疫剂量。该口服苗两次饮水免疫的最适总剂量为每羽５０亿个活菌，最佳间隔时间为４８小时。免疫鸡第二次饮苗后第３天即可产生较好的免疫保护率（７／８）。免疫鸡对异型强毒株Ｐ１０５９（８∶Ａ）、Ｐ２７２３（９∶Ａ）和同型强毒株Ｃ４８－１（５∶Ａ）滴鼻攻击的近期保护率平均为５／１０、６／１０和９／１０。６个月免疫期的平均保护率为７６．７０％（４６／６０），一万多羽鸡田间试验结果表明，免疫后２个月的平均保护率为９３．１０％（２７／２９），６个月的平均保护率为７３．５０％（２５／３４），除对产蛋率有轻度影响（下降１．２７％）外，无其它不良反应。野外大面积使用结果表明，该口服苗不但性能稳定，免疫原性优良，而且安全可靠，适用于鸡口服免疫。     

北疆苜蓿属植物繁育系统的研究

采用等位酶技术，对北疆苜蓿属植物种群的繁育系统进行了研究。结果表明，北疆苜蓿属植物种群为外繁育系统，黄花苜蓿、多变苜蓿、紫花苜蓿基因组成中杂合体过多，均保持较低的自交结实率。参试苜蓿属植物表现某种程度的内繁育衰退现象，地理或生殖隔离等因素可能是导致个别种群出现内繁育衰退现象的原因之一。对北疆苜蓿属遗传资源保护时，既要选择遗传基础丰富的种和种群以保持最多的遗传变异，还要注意选择具有不同等位的种群以保持原有的基因和基因频率。     

一类挖坑机械钻头横向振动系统的最优控制问题

建立了一类挖坑机械钻头横向振动系统的数学模型,并以阻尼函数作为控制变量,研究了该系统的最优控制问题,得出了两个定理。     

ERP系统中设备维修计划的研究

在制造企业设备的使用过程中,设备维修计划成了各种设备管理系统所公认的管理重点,这就更需要设备维修活动在企业内部能形成一个有计划、有步骤的活动。为此,简要介绍了作为ERP管理系统中的子系统一设备管理系统的主要目标,着重讨论了设备管理系统的管理重点一维修计划新思想的形成以及制定维修计划的步骤。     

江西两种棉金钢钻(Earias cupreoviridis Walk.及Earias fabia Stall.)生活习性的初步研究

鼎点金鋼钻和翠纹金鋼钻是江西棉花蕾、鈴期发生普遏为害严重的两种害虫。一般自6月中旬至8月中旬期間,棉田内以鼎点金鋼钻发生数量最大,翠紋金鋼钻較少;8月下旬以后,鼎点金鋼钻迅速下降,翠紋金鋼钻驟然上升,并占绝对优势。鼎点金鋼钻以蛹越冬,年生5—6代,以第2、3代为害最烈;翠紋金鋼钻的越冬問題尚不明确,年生4—5代,以第3、4代为害最烈。在防治上,春季及时处理冬寒菜、蜀葵等植物上为害的鼎点金鋼钻,压低早春基数;在栽培技术上加强棉田管理,促进棉花早熟;在金鋼钻严重发生期内,及时噴射E-605与25％二二三乳剂或6％r可湿性666与25％二二三乳剂混合液,可以有效地減輕两种金鋼钻的为害。     

新疆桑树雌蕊柱头表面超微结构观察与分类的探讨

应用扫描电镜技术观察了和田白桑、伽克1号桑、皮木1号桑的雌蕊形态结构。结果发现和田白桑、伽克1号桑的雌蕊无花柱,柱头内侧表面生有乳头状突起;皮木1号桑雌蕊无花柱,柱头内侧表面生有长毛。根据观察结果,结合其形态特征,和田白桑、伽克1号桑归类于白桑(Morus alba Linn.)较为合适;皮木1号桑有待今后进一步探讨。     

棚菌灵烟剂的研究

筛选出适合配制烟剂的高效药剂,用低成本的供热剂,采用简便、安全的干制法配成复方棚菌灵烟剂,可防治温室、塑料大棚黄瓜、番茄的多种病害,具有高效、经济、低毒、对蔬菜污染小、便于推广应用等特点．     

‘Plantibodies’: a flexible approach to design resistance against pathogens

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.     

甜杂二号甜椒的选育

甜杂二号甜椒为新育成的一代杂种,其植株生长势旺、早熟、丰产、座果率高,连续结实性好,抗TMV病毒能力强.果实绿色灯笼形,味甜质脆,适宜保护地栽培,比对照茄门,同丰37、同丰16、早熟3号等增产31%以上,增值26%以上.1987年通过北京市品种审定.现已成为北京保护地甜椒生产主栽品种之一.全国20个省市均有试种,反映良好.     

保护地番茄新品种苏粉8号的选育

苏粉8号系以GB9736为母本,TM9761为父本配制而成的适合保护地栽培的中熟番茄一代杂种.属无限生长型.果实高圆形,粉红色,果面光滑,果皮厚,耐贮运,品质佳,可溶性固形物含量5.0%,酸甜适中,单果质量200～250 g,产量6 000kg·(667 m2)-1以上.高抗ToMV-0,1株系、叶霉病,抗枯萎病,中抗CMV.已在江苏、山东、四川、浙江等地示范推广逾266.7 hm2.