大蒜新品种成蒜早2号和成蒜早3号的选育

成蒜早2号和成蒜早3号是从温江蒜的自然变异株中,经系统选育而成的早熟新品种.成蒜早2号从播种到蒜薹始收需153～180 d(天),平均单薹质量12.7 g,单头质量10.7 g,多数蒜头由6～9瓣组成,蒜瓣紧密抱握蒜薹轴,一般蒜薹和蒜头产量分别为340和350 kg·(667 m2)-1,抽薹比温江蒜早,抗寒力比温江蒜弱;成蒜早3号从播种到蒜薹始收需153～186 d(天),平均单薹质量和单头质量分别为14.5和12.0 g,多数蒜头由5～8瓣组成.一般蒜薹和蒜头产量分别为320和410kg·(667 m2)-1,抽薹比温江蒜早,抗寒力与温江蒜相当.     

四川‘竹根姜’胚性细胞悬浮系的建立与植株再生

 选用四川省地方品种‘竹根姜’的茎尖组织建立了体细胞胚性细胞悬浮系, 并通过悬浮培养获得了姜再生植株。结果表明悬浮细胞干质量受pH影响。接种量和AgNO₃ 对悬浮细胞的生长都有影响:最佳接种量为1.0% , 最适AgNO₃浓度为6.0 mg·L ^{- 1}。胚性细胞悬浮系增殖后, 接种到前期筛选的成熟分化培养基上获得了再生植株。     

园林布局中必然性和偶然性要素的辩识与运用

阐释了园林构成要素中的必然性要素和偶然性要素及其相互关系与运用意义；以昆明世博园中“齐鲁园”为例，辩识了“齐鲁园”中必然性与偶然性园林要素，分析了诸要素在特定园林环境布局中的运用。     

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.     

浑善达克沙地植物蒸腾特征的研究

采用 L i-1 60 0型气孔仪测定了浑善达克沙地主要建群乡土植物种不同季节的蒸腾速率及其环境因子的日进程 ,结果表明 :浑善达克沙地中榆树、黄柳、圆叶桦、砂杞柳、羊草蒸腾速率的日变化均呈单峰曲线 ,而叉分蓼和楔叶茶子呈双峰曲线。同一种植物在不同样地土壤水分条件下 ,蒸腾速率不同。各植物种蒸腾速率的季节变化明显 ,7、8月份蒸腾速率高 ,6月份最低。各植物种中 ,黄柳为低蒸腾植物。回归分析表明 ,影响蒸腾速率最主要的因子为光照强度 ,其次为气温和空气相对湿度 ;蒸腾速率与各因子呈线性相关 ,其关系可用方程 Tr=a+b RH+c T+d Q表示。通过蒸腾速率的比较 ,提出了浑善达克沙地植被恢复应以建立“人工疏林草原”为主。     

三倍体葡萄新品种‘红标无核''

‘红标无核'是以二倍体‘郑州早红'×四倍体‘巨峰'育成的三倍体无核葡萄品种.果实极早熟,比‘巨峰'早熟30 d,果粒大,紫黑色,无核,果肉较脆,品质上等,可溶性固形物含量15.4%,结实力强,丰产,抗病,适应范围广,适宜鲜食和加工罐头.     

Effect of antifibrotic herb serum on rat hepatic stellate cell proliferation and collagen synthesis

AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16, 8, 4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro. Then we mensurated the radioactivity of HSC admixed with [[³H]H]proline and [[³H]H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0.5, 1, 2 h after intragastrical infusion inhibited HSC proliferation (P＜0.05), and the serum collected 1 h after intragastrical infusion had the strongest effect (P＜0.05). Portal serum decreasea collagen synthesis (P＜0.05), but circumferential serum had no effect (P＞0.05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action.     

土壤湿度对三氮苯类除草剂药效的影响及优化方案

以稗草为生物测定材料,运用二次正交旋转组合设计,以土壤湿度和除草剂用量二因子为决策变量,对稗草的抑制率为目标函数,研究土壤湿度对三氮苯类除草剂药效的影响。结果表明,适当的土壤水分是三氮苯类除草剂发挥药效的重要因素,药效随土壤湿度的提高而提高。不同的土壤湿度对不同除草剂药效影响各异,高湿条件下,湿度差异对药效影响大小依次为嗪草酮、西草净、扑草净、莠去津,低湿条件下则相反。除草剂用量与土壤湿度存在最佳发挥药效的组合。     

氟虫腈(锐劲特)对水稻害虫防治效果及增产效应

为了阐明新一代杀虫剂氟虫腈(锐劲特)对二化螟、三化螟、稻纵卷叶螟的防治效果及应用前景,进行了水稻盆栽试验、田间防效试验,并对施用锐劲特后水稻叶绿素含量、功能叶叶面积和产量进行了测定。结果表明,锐劲特施药后13天接虫的三化螟幼虫死亡率仍高达94.44％。在田间防效试验中,每公顷施用锐劲特80％WG有效成分24g防治二化螟,防止枯心效果达97.62％,防止白穗效果达94.44％,害虫死亡率达96.15％;每公顷施用锐劲特5％SC有效成分22.5g防治晚稻三化螟,保苗效果可达95.91％,害虫死亡率达99.04％;每公顷施用锐劲特5％SC有效成分30g防治稻纵卷叶螟,保叶效果达97.88％。叶绿素含量比对照增加32.4％;每公顷施用锐劲特80％WG有效成分36g时,功能叶叶面积增加16％～27.5％,并且杂交稻博优湛19比常规稻早糯3321和早熟早籼815对锐劲特更敏感。每公顷施用锐劲特5％SC有效成分22.5～37.5g时,水稻株高增加4.0～6.1cm,每稻丛穗数增加2.6％～31.18％,结实率提高19.43％～33.97％,水稻产量增加21.58％～28.19％。     

番木瓜环斑病毒株系的分子生物学方法鉴定

 以PRSV株系特异性引物对PRSV的PRSV126(PRSV日本分离物)、Ys、Vb和Sm等株系进行RT-PCR方法鉴定,引物PR21/PR22能把Ys从Vb和Sm中鉴定出来,PR300/PR301则能把Vb从Ys、Sm和PRSV126中鉴定出来;用限制性内切酶Hae Ⅱ、Sau3A I和Hinf I对PRSV的PRSV126、Ys、Vb和Sm等株系进行单酶切RT-PCR-RFLP分析,Hinf I能把PRSV126与Ys、Vb和Sm鉴别开来,Sau3A I能把Ys与Vb和Sm鉴别开来,Hae Ⅱ则能把Ys与PRSV126、Vb和Sm鉴别开来;以P1/P2为引物,对Vb、Ys和Sm株系进行RT-PCR-RFLP-SSCP分析,结果能一次把三者较好地区别开来。