江西地区鸭疫里氏杆菌的鉴定及药敏试验

从江西部分地区的25份临床疑似鸭疫里氏杆菌(RA)病例中分离到19株RA。生化特性测定结果表明,19个RA菌株的过氧化氢酶、尿素酶均为阳性,除1株发酵葡萄糖、1株发酵麦芽糖外均不发酵各种糖类,5个RA菌株能液化明胶,15个RA菌株氧化酶阳性,不产生H2S,不还原硝酸盐,吲哚试验、MR试验、VP试验均阴性;80%的菌株对头孢曲松、头孢哌酮、头孢唑啉、罗红霉素等高度敏感,而对阿米卡星、庆大霉素、复方新诺明、卡那霉素、青霉素等耐药。     

寄生虫细胞因子免疫的研究进展（一）

寄生虫在入侵机体时必须具备逃避机体免疫,破坏宿主免疫屏障才能感染,这种入侵过程必然和宿主机体的免疫系统发生联系,不但使宿主的先天免疫细胞功能缺失,而且获得性免疫也受到抑制。寄生虫感染均不同程度地伴随免疫损伤,宿主在感染寄生虫后产生的免疫应答中,细胞因子作为免疫过程中的介质起到关键作用,主要是调节机体细胞免疫抗虫,保护机体不被感染,其中巨噬细胞是主要功能的承担者。本文将阐述细胞因子在寄生虫感染过程中所发挥的免疫作用,为解决寄生虫感染的防控问题奠定基础,同时为寄生虫的研究提供新的思路。     

传染性法氏囊病病毒（IBDV）人工感染SPF鸡的病理学观察

取３２０只３０～４０Ｈ龄ＳＰＦ鸡，经滴鼻和点眼接种传染性法氏囊病病毒（ＩＢＤＶ），接种后２４～７２ｈ出现死亡，病死率５１．２５％，死亡鸡剖检变化主要为法氏囊肿胀、出血，甚至呈紫葡萄样，脾脏肿大、出血，骨骼肌出血，腺胃肌胃交界处出血。病理组织学变化，呈现以法氏囊淋巴滤泡内髓质淋巴细胞坏死为主的特征性病变，并可在巨噬细胞浆内发现病毒包涵体。电镜观察，在法氏囊内淋巴细胞、异染性细胞、巨噬细胞浆内，见大量晶格状排列的病毒粒子和包涵体，表明ＩＢＤＶ首先损害法氏囊淋巴滤泡髓质内未成熟的Ｂ淋巴细胞，病毒在淋巴细胞内以包涵体方式增殖。     

退化草地土壤生化活性研究

通过对三种不同退化程度的退化草地土壤生化活性进行测定和相关性分析，结果表明草地退化后其土壤生化活性受到不同程度的影响，表现为土壤生化活性随着退化程度增高而减小，但差异并非都达到统计学上的显著性，而土壤生化活性间的相关性则显著地与草地退化程度有关，说明退化草地土壤微生物间的协调作用受到显著影响，从而影响土壤氮素和碳素代谢途径。     

Characterization of porcine circovirus type 2 (PCV2) infection in swine lymphocytes using mitogen-stimulated peripheral blood lymphocytes from healthy PCV2-carrier pigs

Information regarding the susceptibility of swine lymphocytes to PCV2 is rather limited. To further explore and characterize the PCV2 infection in swine lymphocytes, an in vitro model using concanavalin A (Con A)-stimulated peripheral blood lymphocytes (PBLs) obtained from clinically healthy PCV2-carrier pigs was introduced. It was found that the PCV2 antigen-containing rate was below 2% in PBLs from healthy PCV2-free pigs following treated simultaneously with Con A and PCV2. However, significantly higher PCV2 antigen- and nucleic acid-containing rates could be seen in Con A-stimulated PBLs from clinically healthy PCV2-carrier pigs. Prior to Con A treatment, both of the PCV2 antigen- and nucleic acid-containing rates in PBLs from healthy PCV2-carrier pigs were less than 1%; however, they reached 22.1+/-5.7% by flow cytometry and 27.1+/-6.5% by in situ hybridization, respectively, at 4-day post-incubation with Con A. Phenotyping of PCV2 antigen-containing cells revealed that PCV2-positive cells could be detected in both T and B lymphocyte populations within which IgM-positive B lymphocytes appeared to have a relatively higher positive rate. The Con A-stimulated PBLs also displayed a significantly higher viral load by the measurement of either PCV2 DNA copy number or viral titer when compared with the non-treated PBLs from healthy PCV2-carrier pigs. The results indicate that PBLs, especially IgM-bearing B lymphocytes, are indeed susceptible to PCV2 infection and PCV2 is capable of replicating in dividing lymphocytes. This activation-induced replication may explain in part the pathogenesis of lymphoid depletion in PMWS-affected pigs.     

Use of monoclonal antibodies for analyses of Coxiella burnetii major antigens

Monoclonal antibodies (MAbs) to major antigens of Coxiella burnetii were produced. Some of the MAbs to a 62-kDa protein antigen, peptidoglycan protein complex and lipopolysaccharide (LPS) O-chains reacted with other bacteria whereas none of the MAbs to outer membrane proteins and LPS outer-core did. The LPS outer-core and OMPs may be useful antigens for specifically detecting antibodies to C. burnetii.     

北方塑料暖棚牛舍的设计

本文采用冬季太阳高度角最小,夏季太阳高度角最大,太阳高度角随纬度变化而变化这一原理,确定牛舍棚面闻的计算公式为C=B-h(B：为50“临界值,h为太阳高度角),在舍外温度连-20℃～-30℃时夜间舍内温度达7℃～8℃以上。     

加入WTO对重庆市粮食产业发展的影响与对策研究

通过对中国加入WTO后在粮食产业发展上面临的国内外形势的比较分析，指出了我国粮食产业发展在加入WTO的新形势下如何发展的问题，同时结合重庆市粮食生产情况，为重庆市粮食产业发展提出了一系列的建设性意见和对策，以应对加入WTO对我国及重庆市粮食产业带来的冲击，对把握重庆市粮食产业的发展方向有一定的指导意义。     

公安部向内蒙古六十年大庆安保工作赠送警犬

【背景说明】内蒙古自治区成立于1947年,是在中国共产党领导下,新中国成立的我国第一个少数民族自治区。2007年是内蒙古自治区成立60周年,届时将举行隆重的庆祝活