三种复方对雏鸡柔嫩艾美耳球虫病的疗效试验

作者选用20日龄雏鸡50只,随机分为5组每组10只。设3个感染给药组,1个感染不给药组和1个不感染不给药组。感染组人工接种柔嫩艾美耳球虫孢子化卵囊5.0×10~4/羽。治疗采用如下三种复方:氨丙啉+SQ各180ppm,混饮;TMP+SQ(1:3.25)250ppm,混饲;乙胺嘧啶+SQ(1:5)200ppm,混饲。这些组方均可完全控制因球虫病而引起的死亡。在增重上,前两个用药组与不感染不给药对照组相比差异不显著(P>0.05);后一组方疗效虽高,但对增重有明显的抑制作用(P<0.01)     

乌梁素海环境与资源地理信息系统空间数据库设计

运用数据库技术与地理信息系统技术,通过分析乌梁素海的历史监测数据,应用Superm ap2003地理信息系统软件作为建立数据库的操作平台,通过对基础资料的矢量化,将所得的综合点、线文件进行编辑,误差校正后,分层形成图形文件,将图形文件与属性文件相联接,再通过投影转换和数据格式转换,形成乌梁素海环境与资源地理信息系统空间数据库,从而为今后的空间分析和多用途的开发应用提供了重要的基础数据资料。     

Development and Evaluation of Codon-modified cry V Constructs in Cultivated Potato (Solanum tuberosum L. ) for Control of Potato Tuber Moth

A cryV gene,specifically toxic to Lepidoptera and Coleoptera,was incorporated into binary vectors with different promoters and the presence or absence of the β-glucuronidase(gus) reporter gene.These constructs were integrated into potato cv.Spunta by Agrobacterium tumefaciens-mediated transformation.Highest expression of cryV gene,determined by mRNA levels and insect mortality,was obtained using the CaMV 35S promoter without the gus gene configuration.Detached leaf and tuber bioassays showed a mortality rate of up to 83% and 100%,respectively,for potato tuber moth(Phthorimaea operculella Zeller) in the transgenic lines.Our results demonstrated that the presence of the gus gene negatively affects the expression level of the cryV gene.Bt expression was also facilitated by using the(ocs)3 mas super promoter,whereas the Bt expression regulated by the patatin promoter(tuber-specific) was too low to have any effect upon the mortality of potato tuber moth.These results represent significant improvement in the level of host plant resistance for the control of potato tuber moth via Bt transgenes.     

6110ZL增压中冷柴油机工作过程模拟计算

根据６１１０ＺＬ增压中冷柴油机工作过程的特点，建立了该发动机的数学模型，提出了进气终了状态估算方法及公式。提出了半涡轮叶轮计算方法，使单进口涡轮机一元流分析法得以在双进口无叶涡壳涡轮机上应用。编制出模拟计算软件     

半干旱地区灌木树种造林密度的试验

通过刺槐、沙棘、二色胡枝子、柠条和毛条等灌木树种造林密度的试验,在干旱和半干早地区,刺槐林(1×1)米的密度,5年内可丰产并提早发挥水土保持效益;雨水正常,此密度的丰产期更长。沙棘林以(1×1)和(1×1.5) 米的密度较适宜。二色胡枝子林以(0.3×1)米的密度,4年生以前可以,第5年要视降水情况;(1×1)米的密度可以继续发展。柠条分枝多,造林以(0.5×1.5)米的密度较适宜。毛条分枝少,(条播×1.5)米的密度比(0.5×1.5)米的密度产量高,生长也好。     

恶臭假单胞菌(Pseudomonas putida)GM6的聚磷特性研究

从城市污水处理厂好氧池活性污泥中分离获得一株高效聚磷菌株GM6，经生理生化和16SrDNA初步鉴定为恶臭假单胞菌（Pseudomonas putida）。GM6生长pH在5．5至8．5之间，最适生长pH为6．5；当pH值为7．0时，聚磷能力最强，pH值小于5．5或大于7．5时，除磷能力明显下降。通气量试验表明，装液量对GM6的生长影响较小；装液量为100ml时，磷的去除效果最好，当装液量大于150ml时磷的去除效果变差。GM6的最适生长温度为27℃，当温度小于5℃或大于37℃时生长较慢；当温度为20℃其除磷效果最好，温度大于33℃或小于5℃时，除磷效果明显变差。好氧条件下GM6在合成废水、MOPS培养基、LB及YG培养基中培养时磷的绝对去除量分别为9．87、12．1、87．3和67．1mgL^-1，磷的去除率分别为96．6％、85％、71．6％和63％，磷的绝对去除量和去除率远高于E．coli。好氧培养时菌体吸磷能力测定结果表明，GM6在合成废水、MOPS、LB及YG培养基中培养24h的菌体干重含磷量在6．80％～9．32％之间，而对照菌体含磷量在0．98％～2．31％之间，聚磷菌的聚磷效果远高于对照菌。用序批式反应器对GM6进行厌氧好氧纯培养时，厌氧末的上清液磷浓度为16．8mgL^-1，CODCr为230mgL^-1，放磷速率为4．5mgL^-1h^-1；好氧末的上清液磷浓度为2．56mgL^-1，CODCr为40mgL^-1，好氧吸磷速率为2．73mgL^-1h^-1，菌株GM6表现出了明显的好氧吸磷、厌氧放磷现象，具有聚磷菌的典型特征。     

纤维亚麻新品种黑亚12号选育报告

黑亚12号是2001年2月最新通过黑龙江省农作物品种审定委员会审定推广的纤维亚麻品种，原茎、纤维、种子产量分别为5140.0kg/hm^2 、696.4kg/hm^2和523.9kg/hm^2，分别比对照增产12.6%、20.4%和10.2%。长麻率16.8%，比对照高1.4个百分点。纤维强度259.4牛顿。该品种为优质高产新品种。     

基于嗅觉可视技术的食醋气味表征和区分

利用自主研发的便携式食品气味嗅觉可视检测仪对3种食醋进行了分析,并在实验过程中对传感器制作和图像信号采集进行了优化。结果表明,相机获取图像的整体稳定性优于扫描仪,溶剂挥发时间为15 min时,传感器最为稳定,且与食醋反应可获取最佳响应信号差值;对传感器获取3种食醋挥发气体的响应信号进行主成分分析,再通过线性模型判别,发现独立食醋样本的识别率达到100%。     

STUDIES ON DIFFERENCES OF NITROGEN EFFICIENCY AND ROOT CHARACTERISTICS OF OILSEED RAPE (BRASSICA NAPUS L.) CULTIVARS IN RELATION TO NITROGEN FERTILIZATION

Differences of nitrogen efficiency of oilseed rape (Brassica napus L.) cultivars and their physiological properties were studied in a pot experiment, and the ratio of seed yield with no nitrogen supplied to that with normal nitrogen supply was adopted as a nitrogen efficiency coefficient. Results showed that the nitrogen efficiency coefficient determined for eight oilseed rape cultivars varied from 0.37 to 0.69, the ratio of nitrogen uptake amounts per plant, nitrogen transfer velocity from stems and leaves to seeds, and nitrogen physiological efficiency of oilseed rape cultivars under nitrogen stressed condition differed from with normal nitrogen supply. The higher the nitrogen efficiency of a cultivar, the higher the ratio of N uptake in no nitrogen to with N supplied. Under low nitrogen-supplying conditions, high nitrogen efficiency cultivars had longer roots, more lateral roots, higher amounts of reuse of nitrate from stem and leaves, and higher nitrate reductase activities in leaves.