实验动物质量检测要求及方法概述

简述了有关实验动物质量检测方法的种类,重点介绍了寄生虫和微生物(细菌、病毒、真菌)检测的操作方法。     

雄蚕品种引进试验初报

雄蚕品种的引进与产业化推广是今后蚕业发展的方向。通过引进雄蚕品种夏华×平 8、华秋×平30在实验室对比和农村示范试验 ,试验结果表明 :雄蚕品种综合性状总体较优 ,但某些性状如产量、蚕茧解舒率等有待于进一步改良和完善     

鸭病毒性肝炎的诊断与防治

鸭病毒性肝炎是由鸭甲型肝炎病毒引起的一种急性、烈性、高度接触性传染病,由于病毒血清型较多,且易发生变异而难以有效地免疫预防,导致该病一直成为危害养鸭业健康发展的重要疾病。本文就引起鸭病毒性肝炎的病原、流行病学、临床症状、病理特征以及防治措施等方面进行综述,以期为临床鸭病毒性肝炎的诊断与防控提供参考。     

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised aglandular areas called caruncular (C), and intensely glandular intercaruncular areas (IC). In order to better understand the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins (DEPs) (P < 0.05), functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on. Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

寒地养蜂技术经验谈

养蜂是农村一项传统养殖业,由于受当地地理、气候、蜜源等诸多条件的影响,它有别于其它养殖业,尤其在高寒地区更是如此。在此,笔者与蜂友交流高寒地区的养蜂方法,供参考:     

公安部向内蒙古六十年大庆安保工作赠送警犬

【背景说明】内蒙古自治区成立于1947年,是在中国共产党领导下,新中国成立的我国第一个少数民族自治区。2007年是内蒙古自治区成立60周年,届时将举行隆重的庆祝活     

Optimizing the Conditions for In Vitro Maturation and Artificial Activation of Sika Deer (Cervus nippon hortulorum) Oocytes

With the goal of establishing experimental protocols for cloning sika deer, various conditions for in vitro maturation (IVM) and artificial activation of sika deer oocytes were examined. In vitro maturation was evaluated in seven different culture media. The highest rate of oocyte maturation was 75.4% in 10 μg/ml follicle‐stimulating hormone (FSH), 1 μg/ml LH, 0.2 mm cysteamine and 50 ng/ml epidermal growth factor (EGF) after 24 h of IVM. The efficiency after 24 h of IVM did not differ significantly (p > 0.05) from that observed after 20 h. Cysteamine (0.2 mm ) significantly increased the maturation rates after 20 h (from 59.1% to 67.2%, p < 0.05) and after 24 h (from 63.2% to 71.6%, p < 0.05) of IVM. The IVM rates of oocytes collected during the oestrous season (75.4%) and the anoestrous season (23.3%) were significantly different at 24 h. The 20 μg/ml FSH, 2 μg/ml LH, 0.4 mm cysteamine and 100 ng/ml EGF significantly increased the maturation rates (from 23.3% to 54.2%, p < 0.01) at 24 h during the anoestrous season. For the activation experiments, the most effective method was chemical activation [ionomycin + 6‐dimethylaminopurine (6‐DMAP)], which promoted the development of sika deer oocytes to the blastocyst stage (32.4%). Our results indicate that in vitro matured sika deer oocytes are good candidates for parthenogenetic activation and that chemical treatment is needed for relatively efficient activation of the oocytes. These optimized conditions for IVM and parthenogenetic activation may be useful for efforts to restore populations of the endangered sika deer using the somatic cell nuclear transfer technique.     

舍饲1～7日龄公骡驹行为的观察

对舍饲1～7日龄公骡驹的吸乳、偷乳、站立、运动、睡眠、采食、吃粪、踢、恋群、嬉耍、鸣叫、嗅舐等行为进行了观察,初步探讨了这些行为的产生原因和如何利用这些行为制定科学的骡驹培育措施。     

糖尿病的蜂疗医治法

糖尿病是一种慢性代谢内分泌疾病。由于胰岛素分泌减少或不足,引起糖、脂肪、蛋白质代谢紊乱,促使血糖升高,从而出现糖尿病。糖尿病的典型症状是三多一少,即多饮、多食、     

扎龙国家级自然保护区水鸟群落季节性变化调查

对扎龙湿地的水鸟群落进行资源调查,共记录水禽52种,隶属于5目11科。其中古北界种类42种（占82％）,广布种类10种（占18％）,东洋界种类无记录。对结果进行分析发现：扎龙自然保护区水鸟群落种类和数量随着迁徙活动呈现了明显的季节性变化。春季水鸟迁徙时间集中,种群数量变化剧烈,有高峰期;秋季水鸟迁徙时间分散,种群数量变化缓和,无明显的高峰期。