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41.
Frank N Sommardahl CS Eiler H Webb LL Denhart JW Boston RC 《American journal of veterinary research》2005,66(6):1032-1038
OBJECTIVE: To evaluate glucose and lipid metabolism in healthy adult horses administered levothyroxine sodium (L-T4). ANIMALS: 12 healthy adult mares. PROCEDURE: 8 horses received an incrementally increasing dosage of L-T4 (24, 48, 72, or 96 mg of L-T4/d) for weeks 1 to 8. Each dose was provide between 7 AM and 8 AM in the morning grain meal for 2 weeks. Four additional horses remained untreated. Serum concentrations of nonesterified fatty acids, triglyceride (TG), total cholesterol (TC), and very-low-density lipoprotein (VLDL) were measured and composition of VLDL examined in samples obtained between 8 AM and 9 AM at weeks 0, 2, 4, 6, and 8. Glucose dynamics were assessed by use of a combined IV glucose-insulin tolerance test (IVGITT) conducted before and at the end of the 8-week treatment period. Data for each combined IVGITT were interpreted by use of the minimal model. RESULTS: Plasma TG, TC, and VLDL concentrations significantly decreased over time in treated horses. At the completion of the 8-week treatment period, mean plasma VLDL concentration was 46% of the mean value for week 0 in treated horses. Insulin sensitivity significantly increased (> 2-fold) in treated horses, but glucose effectiveness and net insulin response were not affected. Levothyroxine sodium significantly increased the rate of insulin disposal. CONCLUSIONS AND CLINICAL RELEVANCE: Administration of L-T4 decreases blood lipid concentrations, improves insulin sensitivity, and increases insulin disposal in horses. Levothyroxine sodium may have potential as a treatment for horses with reduced insulin sensitivity. 相似文献
42.
Eiler H Frank N Andrews FM Oliver JW Fecteau KA 《American journal of veterinary research》2005,66(9):1598-1604
OBJECTIVE: To characterize the physiologic response to i.v. bolus injection of glucose and insulin for development of a combined glucose-insulin test (CGIT) in horses. ANIMALS: 6 healthy mares and 1 mare each with pituitary adenoma and urolithiasis. PROCEDURE: Horses were given a CGIT (glucose, 150 mg/kg; insulin, 0.1 U/kg); results were compared with a singular i.v. glucose tolerance test (GTT; 150 mg/kg) and a singular i.v. insulin sensitivity test (IST; 0.1 U/kg). Healthy horses were also given a CGIT after receiving xylazine and undergoing stress. RESULTS: Physiologically, the CGIT resulted in a 2-phase curve with positive (hyperglycemic) and negative (hypoglycemic) portions; the positive phase came first (250% of baseline at 1 minute). The descending segment declined linearly to baseline by approximately 30 minutes and to a nadir at 58% of baseline by 75 minutes. After a 35-minute valley, a linear ascent to baseline began. Addition of insulin in the CGIT increased glucose utilization by approximately 4.5 times during the positive phase but not during the negative phase. The diseases' effects and experimental inhibition of insulin secretion with xylazine and stress were detectable by use of the 2 phases of the CGIT. Only a single positive phase resulted from the GTT and a single negative phase from the IST CONCLUSIONS AND CLINICAL RELEVANCE: The CGIT resulted in a consistent, well-defined glycemia profile, which can be disrupted experimentally or by a disease process. The CGIT has clinical potential because it provides integrated information and more information than either the singular GTT or IST. 相似文献
43.
Pecinali NR Gomes RN Amendoeira FC Bastos AC Martins MJ Pegado CS Bastos OM Bozza PT Castro-Faria-Neto HC 《Veterinary parasitology》2005,134(1-2):121-130
Toxocara canis is a nematode of the Ascaridae family that normally parasites the small intestine of canid species. Humans are accidentally infected upon ingestion of embryonated eggs, and can manifest several clinical alterations such as fever, hepatomegaly, splenomegaly, respiratory symptoms, muscle pain and anorexia. In the present work, we investigated the kinetics of tissue distribution of L2 larva in lungs, liver, kidney, brain, skeletal muscle and myocardium. Also, we analyzed the blood and bronchoalveolar lavage fluid (BAL) for levels of IL-6, IFN-gamma, eotaxin and Regulated on Activation Normal T Cell Expressed and Secreted (RANTES) in experimental murine T. canis infection. We observed liver, lung and kidney lesions correlated to larva migration as early as the first day of infection. After the seventh post-infection day, larva could also be detected in brain, skeletal muscle and heart, as an indicator of biphasic migration pattern. Increased inflammatory activity was detected in BAL and plasma of infected animals, as was an intense eosinophil migration associated with an increase in the levels of all the cytokines studied. In conclusion, our results establish a tight correlation between tissue lesions caused by larva migration and increased plasma levels of pro-inflammatory and eosinophil chemotactic cytokines. Thus, murine T. canis infection may prove to be useful in understanding the role of cytokines in infection. 相似文献
44.
45.
Rock outcrops in the boreal forest of Québec can show either of two different states: a forested state with >25% tree cover,
and an unforested state (<25% tree cover). We tested three different hypotheses that might explain the co-occurrence of forested
and unforested rock outcrops: (1) differences in bedrock geology, with unforested outcrops associated to bedrock types inimical
to tree growth; (2) unforested outcrops as recently disturbed sites undergoing secondary succession towards a forested state;
(3) unforested outcrops as an alternative stable state to forested outcrops, induced by post-fire regeneration failure. Digitized
forest inventory maps were used along with bedrock geology maps and time-since-fire maps to compare forested and unforested
outcrops for bedrock geology type and date of the last fire. Field surveys were conducted on 28 outcrops (14 forested, 14
unforested) to gather information regarding tree species composition and site characteristics (thickness of the organic matter
layer, percent cover of lichens, mosses and ericaceous shrubs). None of the three hypotheses explain the co-occurrence of
forested and unforested rock outcrops in the boreal forest of Québec. Both outcrop types occur on the same bedrock geology
types. Unforested outcrops are not recently disturbed sites in early-successional states, as no clear distinction could be
made in tree species composition and date of the last fire between the two outcrop types. Forested and unforested outcrops
are not alternative stable states, as unforested outcrops are unstable and cannot maintain themselves through time in the
prolonged absence of fire. Hence, unforested rock outcrops could be viewed as degraded, diverging post-fire types maintained
by the late Holocene disturbance regime, characterized by high fire frequencies. 相似文献
46.
47.
Frederick Cubbage Gustavo Balmelli Adriana Bussoni Elke Noellemeyer Anibal N. Pachas Hugo Fassola Luis Colcombet Belén Rossner Gregory Frey Francis Dube Marcio Lopes de Silva Hayley Stevenson James Hamilton William Hubbard 《Agroforestry Systems》2012,86(3):303-314
Silvopasture systems combine trees, forage, and livestock in a variety of different species and management regimes, depending on the biophysical, economic, cultural, and market factors in a region. We describe and compare actual farm practices and current research trials of silvopastoral systems in eight regions within seven countries of the world: Misiones and Corrientes provinces, Argentina; La Pampa province, Argentina; northwestern Minas Gerais, Brazil; the Aysén region of Patagonia, Chile; the North Island of New Zealand; the Southeast United States; Paraguay; and Uruguay. Some countries use native trees and existing forests; some use plantations, particularly of exotic species. Natural forest silvopasture systems generally add livestock in extensive systems, to capture the benefits of shade, forage, and income diversification without much added inputs. Plantation forest systems are more purposive and intensive, with more focus on joint production and profits, for small owners, large ranches, and timber companies. Trends suggest that more active management of both natural and planted silvopastoral systems will be required to enhance joint production of timber and livestock, achieve income diversification and reduce financial risk, make more profit, improve environmental benefits, and realize more resilience to adapt to climate change. 相似文献
48.
The influence of albumin on the pasting and rheological properties of rice flour was investigated. Albumin was removed from the flour of three rice cultivars (Amaroo, Opus, and Langi) by water extraction and the pasting profile of the albumin‐depleted flour was analyzed using the Rapid ViscoAnalyser (RVA). Removal of albumin resulted in a significant (P < 0.5) decline in all the pasting parameters measured. When the extracted albumin was added to pure rice starch, exactly opposite trends occurred. The concentration of albumin in rice starch had a positive linear relationship with all pasting parameters measured. When the gels formed after RVA analyses were analyzed using the TA‐TX2 texture analyzer, the concentration of albumin had a positive linear relationship with hardness, but a near linear negative relationship with adhesiveness. The presence of albumin in rice starch slowed the uptake of water by starch in the initial stages of cooking, but the water uptake accelerated in later stages, and the final water absorption was higher in the samples containing albumin than in pure starch. The water‐soluble nature of albumin suggests that protein‐water‐starch interactions could be responsible for its effect on the physical properties of rice. 相似文献
49.
Yasemin Oner Jorge Hugo Calvo Cengiz Elmaci 《Tropical animal health and production》2013,45(4):947-951
A total of 135 unrelated sheep from nine Turkish native sheep breeds (Dagl?c, Kivircik, Imroz, Chios, Morkaraman, Ivesi, Hemsin, Karayaka and Akkaraman) were investigated to determinate the maternal genetic diversity using a sequence of a 531-bp segment of the mtDNA control region. Analysis of the mtDNA control region sequence revealed 63 haplotypes and 53 polymorphic sites. Haplotype diversity, nucleotide diversity and the average number of nucleotide differences were estimated to be 0.9496?±?0.011, 0.01407?±?0.00060 and 7.456, respectively. The sequence analysis also revealed high level of genetic diversity among the native Turkish breeds. These breeds were grouped into three major maternal haplogroups: A, B and C, with one animal belonging from the Akkaraman breed to the rare haplogroup E. Irregular shape of mismatch distribution of haplogroup C could be an indicator that haplogroup C may represent different haplogroups. Contrarily to previous studies carried out on Turkish native breeds, majority of animals grouped in haplogroup A in the present study. This result and the irregular shape of mismatch curve of haplogroup C indicate that genetic structure of Turkish native sheep breeds could be more complicated than it is thought. 相似文献
50.
Graeme J. Eamens Jocelyn R. Gonsalves Cheryl Jenkins Damian Collins Graham Bailey 《Veterinary parasitology》2013,191(3-4):209-217
Between September 2010 and November 2011, 350 EDTA blood samples were received from 73 Australian cattle herds, as cases suspected to be infected with Theileria orientalis. Beef cattle were predominantly affected, with Angus and Angus-crossbred cattle representing 48% of smear positive samples examined. DNA extracts were tested in conventional polymerase chain reaction (PCR) assays for genes encoding the p32, Ikeda, Chitose and Buffeli major piroplasm surface proteins (MPSP). PCR findings were compared with results of clinical pathology examinations of stained blood smears for parasitaemia and packed cell volume (PCV). PCR testing was much more sensitive than clinical pathology examinations in detecting T. orientalis infections, and concurrent testing of neat and diluted extracts gave significantly more PCR positive results than testing of neat extract alone. Significant associations and correlations were shown between PCR results of p32 and Ikeda assays with PCV levels indicative of anaemia, and with the level of parasitaemia estimated by smears. A high proportion of samples had concurrent Ikeda and Chitose infection, and significantly more clinical cases of theileriosis were associated with the Ikeda MPSP type as the sole infection, compared with sole infection with types Chitose or Buffeli. The findings indicate Ikeda type organisms were significantly associated with clinical parameters of theileriosis in cattle herds in eastern Australia, and that this type is most likely to be responsible for outbreaks of theileriosis experienced in affected Australian herds. In New South Wales, 11 of 14 regulatory districts yielded Ikeda positive samples, with five (Mid-Coast, Cumberland, Central North, Hume and Lachlan) containing 234/307 (76%) of the Ikeda positive samples. 相似文献