胡桃长足象研究初报

胡桃长足象是核桃果实的大害虫,为害损失很大。1975年以来,我们在四川平武地区进行了生活习性观察和防治试验,初步明确了以下几个问题。1.胡桃长足象一年一代。11月份以成虫越冬,次年4月上旬开始活动,为害果、芽、嫩枝、叶柄,5月上旬交尾产卵于果中,一般一果一粒,8月中旬产卵结束,10月陆续死亡。当年成虫6月中旬羽化,进行为害。2.成虫有假死性和向阳性,飞翔力弱,具有与核桃树芽苞相似的保护色。3.防治胡桃长足象,应抓住越冬成虫大量出现和卵孵化盛期时进行。用每毫升含孢量二至五亿的白僵菌液或50%的三硫磷乳油、50%速灭松乳剂、82%磷胺乳剂加水一千倍喷雾,效果良好。     

小麦抗源武汉2号和品冬34的抗条锈性遗传分析

为明确小麦品种武汉2号和品冬34对小麦条锈菌流行小种的抗病性及抗病遗传规律,用小麦条锈菌生理小种CYR29、CYR31、CYR32、CYR33以及致病类型Su11-4、Su11-5、Su11-11、PST-Ch42在苗期接种小麦品种武汉2号和品冬34进行抗病性鉴定,并用武汉2号和品冬34分别与感病亲本铭贤169进行杂交,对F₂群体和F_2：3家系在温室进行苗期遗传分析。结果表明：武汉2号对CYR29和CYR32表现感病,对其它小种和致病型均表现抗病,且对CYR31的抗性由1对隐性基因控制;品冬34对所测试的小种和致病类型均表现高抗,且对CYR32的抗性由1对显性基因控制。     

Artificial Diet Influences Population Growth of the Root Maggot Bradysia impatiens (Diptera: Sciaridae)

In order to investigate the effects of artificial diets on the population growth of root maggot Bradysia impatiens, its population growth parameters were assayed on eight artificial diets (Diet 1, D2, D3, D4, D5, D6, D7, and D8). Results showed that developmental duration from egg to pupa was successfully completed on all eight artificial diets. However, the egg to pupal duration was shortest, while the survival rate of four insect stages was lowest when B. impatiens was reared on D1. When B. impatiens was reared on D7 and D8, the survival rate, female longevity, and female oviposition were higher than those reared on other diets. When B. impatiens was reared on D7, the intrinsic rate of increase (r_m = 0.19/d), net reproductive rate (R₀ = 39.88 offspring per individual), and finite rate of increase (λ = 1.21/d) were higher for its population growth with shorter generation time (T = 19.49 d) and doubling time (Dt = 3.67 d). The findings indicate that the D7 artificial diet is more appropriate for the biological parameters of B. impatiens and can be used an indoor breeding food for population expansion as well as further research. We propose that vitamin C supplement added to the D7 is critical for the improvement of the B. impatiens growth.     

重庆市农业生态系统能流研究

本文对1987年重庆市农业生态系统进行系统分析后得出:单位耕地面积能量输入和输出分别为94.1×10~9J/ha、252.5×10~9J/ha。在投能结构中,非工业能与工业能之比为3.40:1;单位播种面积的工业能投入居全国中等水平,化肥投能增产潜力大,畜禽粪尿能效果显著;能量投入少而复种指数高的县应适当降低复种指数,实行耕地重点投资,以提高单位播种面积产量;畜禽养殖亚系统的生产效率为21.2％;乡村居民在本系统内摄取的食物能为10856.4KJ/人、日,全年烧掉的秸秆相当于整个系统初级生产能量的1/4。     

机器视觉在木材缺陷检测领域应用研究进展

为了提高木材利用率,采用机器视觉来实现木材缺陷快速而稳定的检测,不仅可以克服人工检测效率低、劳动强度大、准确率低等弊端,而且对提高木材加工企业的智能化水平具有重要意义。文中概述了机器视觉检测技术及设备的国内外研究现状,介绍基于机器视觉检测木材缺陷所涉及的相关理论和算法研究,以及相关图像处理算法的优缺点;针对机器视觉应用在木材缺陷检测领域存在的不足,提出机器视觉木材表面检测应进一步向人工智能方向发展,以提高木材缺陷检测效率及准确性。     

冬小麦条锈病内外菌源的原理和效应研究

[研究目的]量化研究小麦条锈病菌源类别、作用和独特性,为防控提供依据.[方法]在易辨期(齐穗扬花期)将病田分类,病菌具潜伏性,在短时间内底叶未发病田和发病田的剑叶发病由外部和内外部菌源侵害引发的,用致控病两种方法研究.[结果]齐穗扬花期之前是以内部菌源作用为主,之后外部菌源作用开始大于内部菌源.齐穗扬花期底叶不发病田控效明显,之后晚发病田控效降低.高温有一定控病效果.发病越早.底部叶片发病越重,内部菌源越多,齐穗扬花期上部叶片发病越重,之后各类病田发病轻重差异减小.[结论]对病田要早期挑治和中期控制,阻止外部菌源的落户和本田菌源的形成,有效减轻后期发病程度,中度以上发病年份.齐穗扬花期普防病田.     

Rapid identification and differentiation of Theileria sergenti and Theileria sinensis using a loop-mediated isothermal amplification (LAMP) assay

The present study developed and validated a species-specific loop-mediated isothermal amplification (LAMP) assay for the rapid detection and discrimination of two benign bovine Theileria species – T. sergenti and T. sinensis. The LAMP assay is inexpensive and easy to perform and involves a rapid reaction-the amplification can be performed in 55 min or 50 min under isothermal conditions of 61 °C or 63 °C, respectively, by employing a set of four species-specific primer mixtures. The results can be checked using agarose gels. The optimal assay conditions, under which the assay exhibited with no cross-reaction with other closely related tick-borne parasites (T. annulata, Babesia bovis, B. bigemina, B. major, B. ovata, B. U. sp., Anaplasma marginale) or between the two Theileria species of interest, was established. The assay is approximately 10-fold more sensitive than the conventional specific PCR assay. The LAMP assay was validated using DNA from 6 standard stocks in the laboratory and was evaluated for its diagnostic utility using blood samples collected from experimentally and naturally infection cattle or yaks in China. These findings indicate that this Theileria species-specific LAMP assay may have potential clinical applications for the detection and differentiation of two benign bovine Theileria species – T. sergenti and T. sinensis, especially in endemic countries.     

基于Deeplabv3+模型的成都平原水产养殖水体信息提取

为应用深度学习和遥感影像实现养殖水体信息的快速提取,以成都平原为研究区,以Sentinel 2A和高分6号多光谱影像为数据源,基于国产开源深度学习平台PaddlePaddle训练Deeplabv3+语义分割模型,构建遥感影像的水体语义分割模型,用于提取成都平原养殖水体信息。Deeplabv3+方法的总体精度和Kappa系数分别达到94.14%和0.88,均高于归一化差分水体指数法和最大似然监督分类法;模型对阴影和建筑物等误分为水体的抑制效果较好,而对小面积和细小线状水体信息的提取则受影像分辨率影响,效果无明显改进;成都平原2018年和2020年养殖水体面积分别为22.3 khm²和28.6 khm²,其验证区青白江区、新津县和广汉市养殖水体面积的泛化提取结果验证误差均≤±10%。该研究结果可为应用深度学习平台建立遥感影像的水体语义分割模型及提取水产养殖水体信息提供参考。     

委内瑞拉链霉菌RL-2活性产物对植物病原真菌的抑制作用及其作用机制

为了探明委内瑞拉链霉菌RL-2在植物病害生物防治中的应用价值,在离体条件下采用生长速率法、活体条件下采用盆栽法研究了RL-2活性产物对多种植物病原真菌的抑制作用。结果表明:在离体条件下,RL-2活性产物对供试的7种病原真菌均有较强的抑制作用,其中对稻瘟病菌的抑制作用最强,EC50为5.35mg/L;在活体条件下,不仅能阻止专性寄生的黄瓜霜霉病病原菌菌丝入侵,而且对已侵入的菌丝有很好的治疗效果。电镜观察结果显示,RL-2活性产物强烈抑制病原菌菌丝生长和孢子萌发,引起菌丝膨大和畸形、孢子萌发率降低、萌发孢子芽管畸形。此外,经RL-2活性物质处理的病原菌接种寄主植物后,其致病性减弱、病斑扩展减慢。