Prevalence of latent cases of Babesia equi infection in some parts of North West India as measured by the capillary agglutination test

The prevalence of Babesia equi infection in north west India was assessed by means of the capillary tube agglutination (CA) test. The particulate antigen used in the test was potent and no cross reaction with other related haemaprotozoa was observed. The serological survey showed that from 323 horses from 3 localities there was an overall incidence of 50.1 per cent. In Haryana the incidence was 38.3 per cent in the 196 horses tested, in Uttar Pradesh it was 47.2 per cent from 72 animals and in Rajasthan it was 96.4 per cent from 55 horses.     

Micronutrients (Fe,Mn, Zn and Cu) balance under long-term application of fertilizer and manure in a tropical rice-rice system

Purpose

The balance of micronutrients in soils is important in nutrient use efficiency, environmental protection and the sustainability of agro-ecological systems. The deficiency or excess of micronutrients in the plough layer may decrease crop yield and/or quality. Therefore, it is essential to maintain appropriate levels of micronutrients in soil, not only for satisfying plant needs in order to sustain agricultural production but also for preventing any potential build-up of certain nutrients.

Materials and methods

A long-term fertilizer experiment started in 1969 at Central Rice Research Institute, Cuttack, Odisha, India. Using this experiment, a study was conducted to analyze the balance of micronutrients and their interrelationship. The experiment was composed of ten nutrient management treatments viz. control; nitrogen (N); N + phosphorus (NP); N + potassium (NK); nitrogen, phosphorus and potassium (NPK); farmyard manure (FYM); N + FYM; NP + FYM; NK + FYM; and NPK + FYM with three replications. Micronutrients in soil (total and available), added fertilizers and organic manures and in rice plant were analyzed. Besides, atmospheric deposition of the micronutrients to the experimental site was also calculated. A micronutrient balance sheet was prepared by the difference between output and input of total micronutrients.

Results and discussion

Application of FYM alone or in combination with chemical fertilizer increased the diethylenetriamine pentaacetate (DTPA)-extractable Fe, Mn and Zn over the control treatment. The treatment with NPK + FYM had the highest soil DTPA-extractable Fe, Mn, Zn and Cu after 41 years of cropping and fertilization. Application of chemical fertilizers without P decreased the DTPA-extractable Zn over the control while the inclusion of P in the fertilizer treatments maintained it on a par with the control. The application of P fertilizer and FYM either alone or in combination significantly increased the contents of total Fe, Mn, Zn and Cu in soil mainly due to their micronutrient content and atmospheric depositions. A negative balance of Zn was observed in the N, NP, NK and NPK treatments, while a positive balance observed in the remaining treatments. The balance of Mn was negative in all the treatments, due to higher uptake by the rice crop than its addition.

Conclusions

Long-term application of chemical fertilizers together with FYM maintained the availability of micronutrients in soil and, thus, their uptake by rice crop.

     

The SnSAG merozoite surface antigens of Sarcocystis neurona are expressed differentially during the bradyzoite and sporozoite life cycle stages

Sarcocystis neurona is a two-host coccidian parasite whose complex life cycle progresses through multiple developmental stages differing at morphological and molecular levels. The S. neurona merozoite surface is covered by multiple, related glycosylphosphatidylinositol-linked proteins, which are orthologous to the surface antigen (SAG)/SAG1-related sequence (SRS) gene family of Toxoplasma gondii. Expression of the SAG/SRS proteins in T. gondii and another related parasite Neospora caninum is life-cycle stage specific and seems necessary for parasite transmission and persistence of infection. In the present study, the expression of S. neurona merozoite surface antigens (SnSAGs) was evaluated in the sporozoite and bradyzoite stages. Western blot analysis was used to compare SnSAG expression in merozoites versus sporozoites, while immunocytochemistry was performed to examine expression of the SnSAGs in merozoites versus bradyzoites. These analyses revealed that SnSAG2, SnSAG3 and SnSAG4 are expressed in sporozoites, while SnSAG5 was appeared to be downregulated in this life cycle stage. In S. neurona bradyzoites, it was found that SnSAG2, SnSAG3, SnSAG4 and SnSAG5 were either absent or expression was greatly reduced. As shown for T. gondii, stage-specific expression of the SnSAGs may be important for the parasite to progress through its developmental stages and complete its life cycle successfully. Thus, it is possible that the SAG switching mechanism by these parasites could be exploited as a point of intervention. As well, the alterations in surface antigen expression during different life cycle stages may need to be considered when designing prospective approaches for protective vaccination.     

Soil loss hinders the restoration potential of tree plantations on highly eroded ravine slopes

Journal of Soils and Sediments - Soil erosion and loss threatens vast tracts of agricultural and non-agricultural land, worldwide. High soil erosion severely affects establishment of vegetation via...     

An Analytical Solution of the One-Dimensional Steady-State Van Genuchten-Based Infiltration Equation for a Heterogeneous Soil with a Root-Water Extraction Function

Eurasian Soil Science - An analytical solution is developed for the one-dimensional steady-state infiltration equation for an inclined Van Genuchten heterogeneous soil with a sink term (i.e., with...     

Influence of nitrogen levels and plant spacing on growth,productivity and quality of two inbred varieties and a hybrid of aromatic rice

A field investigation was conducted at the Indian Agricultural Research Institute's Research Farm during the kharif (wet) seasons of 2002 and 2003 in a split plot design with three replications, consisting of 27 treatments, namely, main plots: three varieties (PRH-10, Pusa Sugandh-3 and Pusa Basmati-1) and three plant spacings (20 × 10, 20 × 15 and 20 × 20 cm²) and sub-plots: three levels of nitrogen (0, 80 and 160 kg N ha^?1). The research results indicated that aromatic rice hybrid PRH-10 produced 33 and 6%, respectively, more grain yield than that of Pusa Sugandh-3 and Pusa Basmati-1. The appreciable higher grain yield of PRH-10 over Pusa Sugandh-3 and Pusa Basmati-1 was due to considerable improvement in most of the yield attributing characters. Application of 160 kg N ha^?1 recorded 23.7 and 26.1% more grain yield over no nitrogen application whereas it was 6.4 and 6.1% more over 80 kg N ha^?1, respectively, during first and second year of the experimentation. Wider plant spacing of 20 × 20 cm² and application of 160 kg N/ha recorded significantly higher hulling, milling and head rice recovery compared to closer spacing and zero nitrogen application.