Gene flow from transgenic roundup-ready soybean to wild soybean

A study was conducted in the field of the Institute of Vegetable Crops, Jiangsu province from July 2000 to August 2003. The transgenic roundup-ready soybean was sown in the middle of the field in a circular manner for 5 circles, with the distance of 3 m, from one circle to another. Then the wild soybean was planted in plots as the rays of the circles in 8 directions （N, E, W, S, NE, NW, SE and SW）, spaced every 5 m until 50 m. Each plot comprised 25 plants. In the second year, the wild soybean seeds from the first year were planted in the field together with the original wild soybean as check. Before flowering time, high concentrations of roundups （about 4-5 times of the normal dose） were sprayed on the plants and the surviving plants were identified. The leaves were taken to the lab for DNA extraction to determine the unique DNA for roundup-ready soybean （CTAB method）. About 2% of the plants survived, but some leaves were yellow. One plant of wild soybean was found to have the roundup-ready gene from the original roundup-ready soybean. The other surviving wild soybeans should also had some fragments of the roundup tolerance gene. However, the DNA bands were not very clear in the PCR map.     

碘类消毒剂存在的问题及对策

对碘类消毒剂存在的问题做了分析和综述，澄清了一些不湛正确的认识，并且对应用中存在的问题提出了解决的对策。对今后的研究和应用具有一定的启发意义。     

应用付里叶变换近红外漫反射光谱法测定谷子中的粗脂肪含量

本文介绍了我们用FTNIRDRS方法对谷物籽粒中蛋白质含量的定量分析的研究工作。预测(谷子、玉米、小麦)方程的相关系数R谷子为0.986,玉米为0.964,小麦为0.972,标准偏差SD,谷子为±0.325,玉米为±0.39,小麦为±0.45。     

火鸡2次免疫后的免疫应答

实验研究了 Lasota 疫苗2次免疫火鸡后,其中枢和外周免疫器官及消化道和呼吸道局部免疫组织中免疫细胞的动态变化。结果表明。2次免疫后,火鸡法氏囊,脾脏、盲肠扁桃体、哈德尔腺和支气管粘膜的浆细胞和淋巴细胞显著增多;胸腺、脾脏、法氏囊与盲肠扁桃体的酯酶阳性 T 细胞数明显升高,巨噬细胞程度不同地增加。ND 强毒攻击后,2次免疫火鸡免疫器官、组织的细胞免疫和体液免疫反应进一步增强,全部免疫火鸡获得免疫保护。对照火鸡则呈现全身和局部细胞免疫及体液免疫机能衰竭,全部发病死亡。     

运用差异显示技术分离水花生在干旱胁迫下表达上调的基因

干旱发生时,水花生的须根将转变成又长又粗的肉质根,这有利于保水和从土壤深处汲取水分以减轻旱害.本研究中采用差异显示技术克隆干旱发生时与水花生根形态发生相关的基因,为进一步通过转基因提高农作物抗旱性奠定基础;共使用15对引物组合,回收到20个可能的在干旱诱导后表达上调的带,其中有一个能被反向northern所证实;而后对其进行了克隆和测序;同源性比对表明它可能是一个新基因的片段.半定量RT-PCR分析进一步证明该基因在干旱和盐胁迫下表达上调.     

人猫共患癣菌病的防治

北京某观赏动物养殖场,养猫120多只,猴子10多只,1986年6月几乎全部发生了皮肤病,在场工作人员都被传染。经流行病学调查,临床症状特点,及用伍德氏灯检查惠毛,显微镜下看到惠毛上有犬小孢子菌,病原菌的分离和培养,人工发病复制实验等,确诊为癣菌病。惠部用克霉唑软膏和癣净治疗,取得了成功。只有人医和兽医密切合作,大力宣传防治此病知识,才能有效地控制其传播。     

试论棉花花芽分化发育的序列性

对4个不同类型品种及不同生态条件下棉花花芽分化发育序列性研究表明:在3500～4000株/亩密度条件下,一级果枝上近主茎的5～6个花芽具较强的生长优势;同位花芽分化期间隔在0.70～0.94之间,邻位花芽分化期间隔在2.03～2.16之间。各果枝近主茎第一花芽具最强生长优势。现蕾后,棉株基部第一、二果枝花芽生长分化变慢;开花后,花芽增长量出现负值。现蕾节以上各分化果枝的花芽分化数有较稳定的序列性,自下而上其分布规律为4,4,3,3,2,2,1,1。     

元谋干热河谷植被恢复造林树种的引种试验研究初报

根据树种选择研究结果，１９９３年在云南元谋引种桉属和金合欢属树种１２个，经两年多的初步观测分析，并与原引种成功的新银合欢比较适应性和速生性，认为赤桉，大叶相思，马占相思可作为元谋干热河谷造林的最优树种，逐步扩大栽培试验，对当地植被恢复适生树种的使用具有一定指导意义。     

Atomic resolution imaging of a carbon nanotube from diffraction intensities

Atomic imaging of three-dimensional structures has required a crystal in diffraction or a lens in electron imaging. Whereas diffraction achieves very high resolution by averaging over many cells, imaging gives localized structural information, such as the position of a single dopant atom. However, lens aberrations limit electron imaging resolution to about 1 angstrom. Resolution is reduced further by low contrast from weakscattering or from the limitations on electron dose for radiation-sensitive molecules. We show that both high resolution and high contrast can be achieved by imaging from diffraction with a nanometer-sized coherent electron beam. The phase problem is solved by oversampling and iterative phase retrieval. We apply this technique to image a double-wall carbon nanotube at 1-angstrom resolution, revealing the structure of two tubes of different helicities. Because the only requirement for imaging is a diffraction pattern sampled below the Nyquist frequency, our technique has the potential to image nonperiodic nanostructures, including biological macromolecules, at diffraction intensity-limited resolutions.     

保护性耕作条件下影响蒸发量因素的试验研究

为分析保护性耕作条件下影响日棵间蒸发量的因素,在一年两熟地区保护性耕作条件下对影响蒸发的各因素进行田间试验研究,对10种保护性耕作模式的各参数与日棵间蒸发量的拟合关系进行分析。结果表明:8种耕作模式条件下日棵间蒸发量与叶面积指数、地表覆盖率有较好的三次函数关系,7种耕作模式下日棵间蒸发量与地表含水率具有较好的三次函数关系,3种耕作模式下日棵间蒸发量与地表温度具有三次函数关系。保护性耕作条件下日棵间蒸发量可近似用叶面积指数、地表覆盖率或者地表含水量计算。