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Ralf ELVERT Nicole EHRHARDT Martin TAUBE Martin KLINGENSPOR Valerie GAILUS‐DURNER Helmut FUCHS Martin Hrabe DE ANGELIS Veronique BLANQUET Gerhard HELDMAER 《Integrative zoology》2006,1(3):122-125
The German Mouse Clinic was established as a phenotyping center to provide the scientific community with systematic standardized phenotyping of mouse models from various genetic backgrounds. We found metabolic phenotypes in nine out of 20 mutant lines screened in a primary screen. Based on these findings, the mutants were analyzed in secondary and tertiary screens. Mice of a sample mutant line, isolated from the ENU‐screen at the National Research Center for Environment and Health in Munich, were found to have lower body weight, consume less food, but have higher ratios of metabolized energy per unit body weight compared with their wild‐type littermates. Basal metabolic rate and heat production were simultaneously increased by 16–18%, whereas body fat content was reduced by 11–16%. The combination of various parameters of energy consumption, expenditure and energy storage illustrate the metabolic demands of the sample mutant mouse line and demonstrate the utility of the powerful phenotyping tool used at the German Mouse Clinic. 相似文献
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EFFECTS OF SEDATION WITH MIDAZOLAM AND BUTORPHANOL ON RESISTIVE AND PULSATILITY INDICES IN HEALTHY DOGS 总被引:1,自引:0,他引:1
ROSA NOVELLAS RAFAEL RUIZ DE GOPEGUI YVONNE ESPADA 《Veterinary radiology & ultrasound》2007,48(3):276-280
Resistive index (RI) and pulsatility index (PI) are indirect measurements of blood flow resistance that may be measured by pulsed wave Doppler ultrasonography. Chemical restrain may potentially alter the indices although it is required to perform ultrasonography in some patients. The purpose of this study was to describe values for both intrarenal and ocular RI and PI within the same subject in clinically normal dogs sedated with a midazolam and butorphanol combination and evaluate if there are any significant changes between sedated and nonsedated dogs. Fifteen healthy Beagle dogs were studied by Duplex Doppler interrogation in interlobar or arcuate arteries of the kidney and long posterior ciliary artery. Pulse rate and systolic blood pressure were also determined. All measurements were recorded before and after the administration of a sedative combination of midazolam (0.2 mg/kg) and butorphanol (0.2 mg/kg). Mean comparison tests (paired t-tests or Wilcoxon's rank-sum test) were used to determine if any significant differences existed between right and left renal values or right and left ocular values. A correlation study (Pearson or Spearman) was applied between RI, PI, and systolic pressure, and pulse rate. RI and PI were significantly higher in sedated Beagles than in unsedated Beagles. There was neither correlation between index and systolic blood pressure nor pulse rate. In conclusion, provided that normal RI and PI increase in sedated animals, then reference ranges should be higher when sedated--healthy or ill--animals are evaluated. 相似文献
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TIZIANA LIUTI JIMMY H. SAUNDERS INGRID GIELEN LIEVE DE RYCKE FRANK COOPMAN HENRI VAN BREE 《Veterinary radiology & ultrasound》2007,48(4):361-367
The aim of the present study was to evaluate to what extent the distal tibia and the trochlear ridges of the talus can be examined with ultrasound (US) in the dog and to establish a protocol for an optimal US examination of these ridges. Six hind limbs of deceased adult mixed-breed dogs were used. In two limbs, needles were placed using US guidance on the trochlea of the talus, just dorsal to and plantar to the distal tibia: one with the tarsal joint in extension and one with the joint in flexion. Then mediolateral (ML) radiographs of both joints were made with the needle in place to determine the percentage of the trochlear ridge of the talus that can be seen using US imaging. An US examination of the tarsal joint was performed on the four other limbs using microconvex (8 MHz) and linear (12 MHz) transducers (Logiq 7) and compound imaging. A three-step protocol was performed including a dorsal approach with the limb extended and the linear transducer (step I), a plantar approach with the limb flexed and the linear transducer (step II), and a plantar approach with the limb flexed and the microconvex transducer (step III). After the US examination, the four limbs were frozen and sectioned, two in a transverse and two in a sagittal plane. Bony structures on the US images were matched with the corresponding anatomic sections. The distal tibia and both trochlear ridges of the talus were easily recognized on the US images using the proposed protocol. When combining the dorsal and plantar approaches, it was possible to visualize up to 75% of the trochlear ridges of the talus in the dog. 相似文献
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C.T. DE WIT 《EPPO Bulletin》1979,9(3):153-153
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Feline leukemia virus: occurrence of viral antigen in the tissues of cats with lymphosarcoma and other diseases 总被引:6,自引:0,他引:6
W D Hardy G Geering L J Old E DE Harven R S Brody S McDonough 《Science (New York, N.Y.)》1969,166(908):1019-1021
Felinie leutkemia viruts antigen is demonstrable by immunodiffusion with rabbit precipitating antiserum to purified felinie leukemia virus. The felinie leukemia virus antigen was found in the tissues of 25 of 33 cats with lymphosarcoma and of 5 of 13 cats with infectious peritonitis. Its presence was correlated with the occurrence of felinie leukemia virus demonstrable by electron microscopy. The one clinically normal cat giving a positive test for feline leukemia virus antigen belonged to a household in which two cats had developed lymphosarcoma. With the exception of a dog with lymphosarcoma induced by feline leukemia virus, the antigent was absent from lymphosarcoma and nonlymphomatous tumors of other species (man, dog, cow, goat, or pig). 相似文献