全文获取类型
收费全文 | 157篇 |
免费 | 6篇 |
专业分类
林业 | 11篇 |
农学 | 26篇 |
基础科学 | 2篇 |
26篇 | |
综合类 | 9篇 |
农作物 | 11篇 |
水产渔业 | 16篇 |
畜牧兽医 | 45篇 |
园艺 | 2篇 |
植物保护 | 15篇 |
出版年
2022年 | 1篇 |
2021年 | 7篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 6篇 |
2016年 | 4篇 |
2015年 | 6篇 |
2014年 | 5篇 |
2013年 | 8篇 |
2012年 | 6篇 |
2011年 | 7篇 |
2010年 | 7篇 |
2009年 | 6篇 |
2008年 | 4篇 |
2007年 | 13篇 |
2006年 | 17篇 |
2005年 | 8篇 |
2004年 | 7篇 |
2003年 | 9篇 |
2002年 | 1篇 |
2001年 | 7篇 |
2000年 | 8篇 |
1999年 | 5篇 |
1998年 | 2篇 |
1993年 | 3篇 |
1991年 | 2篇 |
1989年 | 1篇 |
1988年 | 3篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1979年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有163条查询结果,搜索用时 15 毫秒
31.
Rui Kano Takayuki Yano Kota Nagamatsu Haruhiko Maruyama Hiroshi Kamata Atsuhiko Hasegawa 《Research in veterinary science》2009,87(1):64-66
The effect of down-regulation of Mcl-1 expression by small interfering RNA (siRNA) against the canine Mcl-1 gene on apoptosis was investigated by transfecting CF33 (canine mammary gland tumor cell line) with siRNA using cationic liposomes. The siRNA against canine Mcl-1 increased the rate of apoptotic cells and decreased the numbers of viable cells. Further, sequence-specific down-regulation of Mcl-1 expression was measured by real time-PCR and Western blot analysis. The siRNA directed against the Mcl-1 gene reduced both the mRNA and protein expression in the CF33. Our study suggests the importance of Mcl-1 in canine mammary tumors for inducing apoptosis and reinforces using Mcl-1 as a putative therapeutic target in canine mammary gland tumor. 相似文献
32.
Nardi AR Salvadori MR Coswig LT Gatti MS Leite DS Valadares GF Neto MG Shocken-Iturrino RP Blanco JE Yano T 《Veterinary microbiology》2005,105(3-4):245-249
The culture supernatant of Escherichia coli, isolated from ostriches with diarrhea in Brazil, caused elongation in Vero cell, rounding in Chinese hamster ovary (CHO) cells and a cytoplasmic vacuolation in ostrich embryo fibroblasts (OEF), but it was not cytotoxic for chicken embryo fibroblasts (CEF). These effects were not neutralized by antiserum to cholera toxin. Polymerase chain reaction assays showed that the ostrich E. coli contained the gene encoding (eltII-A), but not those for type 1 heat-labile enterotoxin (eltA), heat-stable enterotoxins (estA, estB), verocytotoxins (stx-I, stx-II), or cytotoxic necrotizing factors (cnf 1, cnf 2). All isolates belonged to serotype O15:H8. The enteropathogenic relevance of LT-II in ostrich diarrhea remains undetermined. 相似文献
33.
Naruto?FuruyaEmail author Fumika?Shimokusuzono Yutaka?Nakamura Kishiko?Nishimura Minoru?Takeshita Nobuaki?Matsuyama Kayo?Manabe Youichi?Takanami 《Journal of General Plant Pathology》2004,70(1):39-44
Crown gall disease of tobacco was found in Iwate Prefecture, Japan in 1995. Ten bacterial isolates, obtained from the galls of tobacco, were identified as Agrobacterium tumefaciens (Smith and Townsend 1907) Conn 1942 biovar 1 based on their ability to induce galls on the 14 tested plants, including tobacco after needle-prick inoculation, and on 12 cultural, physiological, and biological characteristics. The growth of the causal organism was not inhibited in vitro by agrocin of A. radiobacter strain K84. This report is the first on the natural occurrence of crown gall caused by A. tumefaciens on tobacco plants. 相似文献
34.
Jeraj KP O'brien TD Yano BL 《The Canadian veterinary journal. La revue veterinaire canadienne》1983,24(1):20-23
Systemic mastocytoma was diagnosed in a 16 year old cat. Prednisone therapy was initiated but discontinued after 11 months because of development of a duodenal ulcer. Twenty months after the initial diagnosis the cat developed dyspnea, due to enlarged pharyngeal lymph nodes. Euthanasia was performed; systemic mastocytoma and lymphosarcoma were found at necropsy. 相似文献
35.
Production of intergeneric hybrids between Brassica and Sinapis species by means of embryo rescue techniques 总被引:1,自引:0,他引:1
Reciprocal crosses were made to produce intergeneric hybrids among seven species of Brassica and three species of Sinapis
with the aid of embryo rescue techniques. Ovule culture showed better responses in terms of hybrid plant development. Altogether,
hybrids from 8 different combinations were obtained from the crosses between B. campestris var. trilocularis × S. turgida,
B. campestris var. pekinensis × S. arvensis, S. arvensis × B. campestris var. pekinensis, B. oleracea var. alboglabra × S.
alba, B. oleracea var. alboglabra × S. turgida, B. carinata × S. alba, B. carinata × S. arvensis, and B. carinata × S. turgida.
Reciprocal crosses yielded no hybrid except in the combination of S. arvensis × B. campestris var. pekinensis. Among them
hybrids from 6 combinations were established in the glass house. The hybridity of the plants was confirmed by morphological
characters, pollen stainability, chromosome number and by isozyme analysis. Hybrids of four combinations out of six turned
out as true hybrids, one as sesquidiploids and the another one as false hybrid plant.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
36.
S Kawaii Y Tomono E Katase K Ogawa M Yano 《Journal of agricultural and food chemistry》1999,47(7):2509-2512
To eliminate the masking effect by flavonoid glycosides, which comprise approximately 70% of conventionally prepared sample, the readily extractable fraction from Citrus juice, which was prepared by adsorbing on HP-20 resin and eluting with ethanol and acetone from the resin, was subjected to antiproliferative tests against several cancer cell lines. Screening of 34 Citrus juices indicated that King (Citrus nobilis) strongly inhibited proliferation of all cancer cell lines examined. Sweet lime and Kabuchi inhibited three of the four cancer cell lines. In contrast, these samples were substantially less cytotoxic toward normal human cell lines. 相似文献
37.
Hiroshi Oikawa Yukihiko Matsuyama Masataka Satomi Yutaka Yano 《Fisheries Science》2008,74(5):1180-1186
The accumulation of paralytic shellfish poisoning (PSP) toxin in the crab Charybdis japonica was investigated in Kure Bay, when a bivalve Mytilus galloprovincialis, in the same water, showed toxicity caused by PSP toxin. In 2005, 17 specimens of the crab had PSP toxin over 4 MU/g in the
hepatopancreas, and the highest toxicity was at 37.4 MU/g. Since the regulation limit of PSP for crab hepatopancreas was set
in 2004, this is the first observation of regulatory level of PSP toxin in C. japonica. Unlike in the hepatopancreas of the crab, the hazardous level of the toxin was not detected in the muscle of the cephalothorax
and the appendage. The toxin accumulation in the crab was also investigated by feeding toxic mussels to the crab. The crab
retained the toxin mainly in the hepatopancreas, and the ratio of retention in the crab was from 12.9 to 24.6%. The toxin
profiles, shown in the feeding experiments, suggest that the transformation of PSP toxin occurs in the crab because dcGTX2
and dcGTX3 was detected in all crab specimens despite the lack of these analogs in the mussels used as feed. 相似文献
38.
Heat stress causes oxidative stress, which decreases plasma levels of vitamin C, an antioxidative vitamin in pigs and poultry. We investigated the plasma vitamin C concentration in lactating cows exposed to a hot environment temperature. In the first experiment, plasma vitamin C concentrations of 8 lactating cows were measured in summer and autumn. The concentration was found to be significantly lower in summer. The second experiment was performed using 4 lactating cows in a crossover design. The ambient temperature was set at 18 °C in the control treatment and at 28 °C in the heat-stressing treatment. Milk yields and feed intake tended to be lower in the stressing than control treatment. Rectal temperature and respiration rate were significantly (P < 0.05) higher in the heat-stressing treatment. The plasma vitamin C concentration was significantly (P < 0.05) lower in the heat-stressing period. These results indicate that heat stress decreases the plasma vitamin C concentration. Dietary supplementation with vitamin C may be beneficial for lactating cows in hot weather. 相似文献
39.
Nobuya Hishiyama Hideki Kayanuma Tohru Matsui Hideo Yano Tsunenori Suganuma Masayuki Funaba Hiroshi Fujise 《Canadian journal of veterinary research》2006,70(4):305-307
Most mammals, including dogs, synthesize vitamin C in the liver. We measured the plasma concentration of vitamin C to assess the body vitamin C status in 15 dogs with a portosystemic shunt (PSS). The plasma biochemical parameters indicated liver abnormalities in all the dogs. In contrast, the plasma concentration of vitamin C ranged from 2.21 to 9.03 mg/L in the 15 dogs and was below the reference range (3.2 to 8.9 mg/L) in only 2 dogs. These findings suggest that vitamin C status is not impaired in dogs with PSS. 相似文献
40.