富贵竹室内加工技术研究

 设杀菌剂瑞毒霉锰锌、营养液爱多收和促根剂吲哚丁酸不同浓度9个处理组合, 研究其对富贵竹去叶植株修口、顶侧芽生长及生根的影响。结果表明, 瑞毒霉锰锌3水平间修口效果、爱多收3水平间顶侧芽生长、吲哚丁酸3水平间根系生长的差异均极显著; 最佳处理组合, 小竹为0. 8 g/L^-1瑞毒霉锰锌+ 0. 15 mL/L^-1爱多收+ 30 mg/L^-1吲哚丁酸, 大竹为0. 8 g/L^-1瑞毒霉锰锌+ 0. 30 mL/L^-1爱多收+ 30 mg/L^-1吲哚丁酸。     

Differential gene expression of Eph‐ephrin A1 and LEPR‐LEP with high or low number of embryos in pigs during implantation

The objective of this study was to ascertain whether mRNA and protein expressions of implantation‐related genes (erythropoietin‐producing hepatocellular receptor–ligand A1, Eph‐ephrin A1 and leptin receptor–leptin, LEPR‐LEP) differed between pigs with high and low number of embryos, and whether these differences in gene expression might affect embryo implantation. Experimental pig groups (n = 24) for high and low number of embryos were prepared by altering the number of eggs ovulated in pre‐pubertal gilts treated with 1.5 × (High) or 1.0 × (Low) PG600 ([400 IU PMSG + 200 IU hCG]/dose, AKZO‐NOBEL). Gilts expressing oestrus were artificially inseminated twice and maintained in breeding and gestation until the reproductive tract was collected on day 22 of pregnancy. At slaughter, the reproductive tracts from each pregnant gilt from each treatment were immediately processed to collect samples for RNA and protein analysis. Within each gilt, three conceptus points were sampled, one from each horn and then a random conceptus within the tract. At each conceptus point, endometrial attachment site, chorion–allantois and embryo were collected and immediately frozen in liquid nitrogen. Number of corpus luteum (CL) (35.4 vs. 12.6) and total embryo number (18.8 vs. 10.2) were greater in the high‐embryo compared to the low‐embryo group, respectively (p < .05). Real‐time qPCR results showed that Eph‐ephrin A1 mRNA expression was less in the high‐embryo (p < .05) compared to the low‐embryo group. In addition, Western blotting analysis indicated that Eph‐ephrin A1 and LEP protein expression at endometrial attachment site in high‐embryo was less (p < .05) compared to low‐embryo group. It was also noted that mRNA expression of Eph‐ephrin A1 and LEPR‐LEP was greater in pregnant than non‐pregnant gilts (p < .05). Moreover, mRNA expression of Eph‐ephrin A1 (p < .05) and LEPR‐LEP was greatest at endometrial attachment site among all three tissues. There was a positive correlation between expressions of Eph‐ephrin A1, LEPR‐LEP and embryo length with the correlation coefficient 0.31–0.59. For Eph‐ephrin A1, the highest correlation coefficient appeared between Eph A1 expression and normal embryo number, between ephrin A1 expression and embryo length. For LEPR‐LEP, the highest correlation coefficient appeared between LEPR‐LEP expression and ovary weight (0.79 for both, p < .05), followed by embryo length and weight. The results of this study suggest that low expression of Eph‐ephrin A1 and LEPR‐LEP is somehow related to increased embryo number during implantation and that endometrial attachment site might be the main target tissue of these gene products. Yet, the increased expression of Eph‐ephrin A1 and LEPR‐LEP appeared associated with increased embryo growth (length and weight) and ovary weight, Eph‐ephrin A1 and LEPR‐LEP might play roles in the regulation of embryo implantation in pigs.     

紫花苜蓿9个盐响应相关基因表达模式

紫花苜蓿(Medicago sativa)是世界上种植面积最大、应用最广泛的豆科牧草。由于其耐盐性中等,其在我国北方地区的产量和种植受到土壤盐渍化的限制。因此提高紫花苜蓿的耐盐性具有重要的科学和生产意义。为此,以龙牧801紫花苜蓿(M.sativa‘Longmu 801’)为试验材料,采用实时荧光定量PCR技术分析了不同浓度NaCl胁迫下9个盐胁迫蛋白质组筛选出的盐响应相关基因的表达模式。结果显示,处理时间和处理浓度对9个基因的相对表达量均有显著性影响,表明这9个基因均在紫花苜蓿盐胁迫应答中发挥着一定作用。处理1h时,G6PI、ABP19a、Trx-h1、PR bet 1、FBPA、6PGDH和ALDH这7个基因的相对表达量在不同NaCl胁迫浓度处理的紫花苜蓿中均显著上调,RRM和GDPD在NaCl处理2h后开始上调。除G6PI基因,其他8个基因在0.4%NaCl处理下相对表达量显著高于0.2%和0.8%NaCl处理。这些基因参与糖代谢、信号转导和胁迫响应。以上结果表明,紫花苜蓿的耐盐性极其复杂,涉及到多基因的表达和代谢通路的调控。研究结果有助于全面研究并了解紫花苜蓿的盐响应相关基因的表达模式,促进紫花苜蓿耐盐分子育种。     

埋钻事故分析及预防措施

本文对埋钻事故进行分析,提出了预防措施,结合工程实例,对埋钻事故分析更加清晰.     

杉木不同世代不同类型种子园遗传改良增益研究

以浙江省17个无性系种子园为研究对象,连续2年采种育苗造林,进行2次4地点5区组的种子园子代测定试验,测定树高、胸径、材积、冠幅、侧枝等8个性状.结果表明:从未经遗传改良普通林分种子到1代、1.5代、2代种子园,子代生长增益是逐代增长的,但世代之间增幅明显下降.优良双系种子园子代生长增益最大,优良种源种子园次之.1代、1.5代和2代种子园,种源种子园,双系种子园的子代生长,在6年生时,树高生长现实增益分别为4.35%、6.04%、7.88%、12.02%和12.02%,遗传增益依次为3.90%、5.41%、7.06%、10.77%和10.77%;胸径现实增益为7.60%、9.68%、12.08%、15.05%和19.70%,遗传增益为7.07%、9.00%、11.23%、14.00%和18.32%;材积现实增益为20.65%、27.47%、32.14%、39.93%和46.14%,遗传增益为16.97%、22.58%、26.42%、32.82%和37.93%.为持续获得高增长的遗传改良效果,在高世代种子园建设中,应充分发掘和优先利用优良种源的优质种质资源;在提升育种群体遗传水平,保持遗传基础多样性的同时,建立双系种子园,实现工业原料林用种的品种化,应是今后的一个重要发展方向.     

浅议审计部门在建设项目全过程造价控制中的作用

审计部门如何发挥自身监督作用,合理有效地控制工程造价,直接关系到企业的健康发展。建设项目全过程造价控制已经成为审计实践的发展方向,并有助于提高企业建设项目的管理水平,发挥建设资金的使用效益。     

陇中黄土高原半干旱区9个燕麦品种籽粒产量及其营养价值研究

本研究对9个燕麦(Avena sativa)品种的主要农艺性状、种子产量和籽粒营养品质进行了比较,以期筛选出适宜在陇中黄土高原半干旱区生产利用的燕麦品种。结果表明：供试燕麦品种均属于中熟型,在当地均能成熟;9个燕麦农艺性状、种子产量及籽粒营养品质之间存在显著差异(P<0.05),‘迪燕1号’的株高和穗长最高,分别为150.00cm和30.50cm,但种子产量最低,为1734.20kg·hm^-2,穗粒数和穗粒重均以‘魏都莜5号’最高,分别为128.4粒和2.84g,‘晋燕17号’的种子产量和淀粉含量最高,分别为2500.00kg·hm^-2和53.68%,但粗蛋白含量最低,仅为15.95%,‘张莜8号’的千粒重最高,为28.20g,粗蛋白含量以‘张莜9号’最高,达18.75%,‘坝莜18号’的β-葡聚糖和粗脂肪含量最高,分别为4.37%和6.35%。应用灰色关联度理论方法对9个燕麦品种的主要农艺性状、种子产量与籽粒营养品质指标进行综合分析得出,‘晋燕17号’‘魏都莜5号’‘迪燕1号’综合性状较好,适宜在陇中黄土高原半干旱区种植及推广。     

在适应变革中实现平稳转型——在华西希望集团饲料经营分析会上的讲话

2004年是我国饲料行业具有转折意义的一年。经过这一年的市场利好,饲料行业出现了长达几年的严冬。据统计,截止于2006年底,全国已有5000多家中小饲料企业停产。2007年已经过去快半年了,由于受整个行情的影响,大企业的销量及利润大幅下滑,相当一部分企业出现亏损。[第一段]     

毛竹林内夏季气温变化的特征比较

定位研究位于浙江省富阳市的不同结构毛竹林,2003 年夏季林内气温的日变动状况。结果表明:毛竹林内以日最低气温的变异幅度最大,日最高气温次之,日均气温最小;毛竹纯林内日均最低气温21.20℃,低于竹阔混交林1.32℃,日均最高气温为29.67℃,低于竹阔混交林1.03℃。毛竹林内最低气温主要出现在凌晨 4:00￣6:00,毛竹纯林调节林内最低气温的效应值与栎林相似约-0.3℃,竹阔混交林效应值约1.25℃。方差分析显示毛竹纯林、竹阔混交林、茶园3类林分间的日均气温差异极显著,日最低气温差异显著。