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121.
Florian Wichern Darima Andreeva Rainer Georg Joergensen Yakov Kuzyakov 《植物养料与土壤学杂志》2011,174(5):732-741
To investigate C and N rhizodeposition, plants can be 13C‐15N double‐labeled with glucose and urea using a stem‐feeding method (wick method). However, it is unclear how the 13C applied as glucose is released into the soil as rhizorespiration in comparison with the 13C applied as CO2 using a natural uptake pathway. In the present study, we therefore compared the short‐term fate of 14C and 15N in white lupine and pea plants applied either by the wick method or the natural pathways of C and N assimilation. Plants were pulse‐labeled in 14CO2‐enriched atmosphere and 15N urea was applied to the roots (atmosphere–soil) following the natural assimilation pathways, or plants were simultaneously labeled with 14C and 15N by applying a 14C glucose–15N urea solution into the stem using the wick method. Plant development, soil microbial biomass, total rhizorespiration, and distribution of N in plants were not affected by the labeling method used but by plant species. However, the 15N : N ratio in plant parts was significantly (p < 0.05) affected by the labeling method, indicating more homogeneous 15N enrichment of plants labeled via root uptake. After 14CO2 atmosphere labeling of plants, the cumulated 14CO2 release from roots and soil showed the common saturation dynamics. In contrast, after 14C‐glucose labeling by the wick method, the cumulated 14CO2 release increased linearly. These results show that 14C applied as glucose using the wick method is not rapidly transferred to the roots as compared to a short‐term 14CO2 pulse. This is partly due to a slower 14C uptake and partly due to slow distribution within the plant. Consequently, 14C‐glucose application by the wick method is no pulse‐labeling approach. However, the advantages of the wick method for 13C‐15N double labeling for estimating rhizodeposition especially under field conditions requires further methodological research. 相似文献
122.
Response of sediment biofilm to increased dissolved organic carbon supply in groundwater artificially recharged with stormwater 总被引:1,自引:0,他引:1
Arnaud Foulquier Florian Mermillod-Blondin Florian Malard Janine Gibert 《Journal of Soils and Sediments》2011,11(2):382-393
Purpose
Best management practices encompass diverse artificial groundwater recharge (AGR) systems that heavily rely upon the capacity of the soil and vadose zone to retain large quantities of organic matter generated during stormwater runoff on urban catchments. However, the supply of stormwater-derived dissolved organic carbon (DOC) at the water-table region of aquifers can enhance the rate of biogeochemical processes by fueling heterotrophic microbial metabolism. This study examined changes in the abundance and activity of sediment biofilm in response to increased DOC supply at the water table of an urban aquifer intentionally recharged with stormwater. Changes in microbial abundance and activity under field conditions were compared with those measured in laboratory slow filtration columns supplied with an easily biodegradable source of DOC. 相似文献123.
Diskin R Scheid JF Marcovecchio PM West AP Klein F Gao H Gnanapragasam PN Abadir A Seaman MS Nussenzweig MC Bjorkman PJ 《Science (New York, N.Y.)》2011,334(6060):1289-1293
Antibodies against the CD4 binding site (CD4bs) on the HIV-1 spike protein gp120 can show exceptional potency and breadth. We determined structures of NIH45-46, a more potent clonal variant of VRC01, alone and bound to gp120. Comparisons with VRC01-gp120 revealed that a four-residue insertion in heavy chain complementarity-determining region 3 (CDRH3) contributed to increased interaction between NIH45-46 and the gp120 inner domain, which correlated with enhanced neutralization. We used structure-based design to create NIH45-46(G54W), a single substitution in CDRH2 that increases contact with the gp120 bridging sheet and improves breadth and potency, critical properties for potential clinical use, by an order of magnitude. Together with the NIH45-46-gp120 structure, these results indicate that gp120 inner domain and bridging sheet residues should be included in immunogens to elicit CD4bs antibodies. 相似文献
124.
Creis Bendelac Emeline Delage Ludovic Vallet Laurent Leblanc Catherine Inken Kruse Ar Gall Erwan Weinberger Florian Potin Philippe 《Marine drugs》2021,19(4)
Mechanisms related to the induction of phlorotannin biosynthesis in marine brown algae remain poorly known. Several studies undertaken on fucoid species have shown that phlorotannins accumulate in the algae for several days or weeks after being exposed to grazing, and this is measured by direct quantification of soluble phenolic compounds. In order to investigate earlier inducible responses involved in phlorotannin metabolism, Fucus vesiculosus was studied between 6 and 72 h of grazing by the sea snail Littorina littorea. In this study, the quantification of soluble phenolic compounds was complemented by a Quantitative real-time PCR (qRT-PCR) approach applied on genes that are potentially involved in either the phlorotannin metabolism or stress responses. Soluble phlorotannin levels remained stable during the kinetics and increased significantly only after 12 h in the presence of grazers, compared to the control, before decreasing to the initial steady state for the rest of the kinetics. Under grazing conditions, the expression of vbpo, cyp450 and ast6 genes was upregulated, respectively, at 6 h, 12 h and 24 h, and cyp450 gene was downregulated after 72 h. Interestingly, the pksIII gene involved in the synthesis of phloroglucinol was overexpressed under grazing conditions after 24 h and 72 h. This study supports the hypothesis that phlorotannins are able to provide an inducible chemical defense under grazing activity, which is regulated at different stages of the stress response. 相似文献
125.
Jóska Gerendás Florian Heuser Burkhard Sattelmacher 《Journal of plant nutrition》2013,36(9):1499-1516
Fried potato products may accumulate substantial amounts of acrylamide due to high precursor contents, namely reducing sugars and asparagine. In a two-factorial experiment increasing N supply, increased the contents of reducing sugars in most cases, and resulted in higher contents of free amino acids. α -amino-N, which was tightly correlated with the contents of free amino acids, can be regarded a suitable rapid test for free asparagine for a given variety. Increasing K addition always raised the citrate contents, but lessened the contents of reducing sugars. Selected treatments were processed into French fries. Highest acrylamide contents were observed in tubers grown with high N and inadequate K supply, which also contained the highest contents of precursors. The experiment clearly demonstrates that nutrient supply has significant impact on the contents of acrylamide precursors and thus for the acrylamide formation during frying. 相似文献
126.
Sophie Cornu Saskia Keesstra Antonio Bispo Maria Fantappie Fenny van Egmond Bozena Smreczak Rafał Wawer Lenka Pavlů Jaroslava Sobocká Zsófia Bakacsi Kinga Farkas-Iványi Sándor Molnár Anders Bjørn Møller Sevinc Madenoglu Dalia Feiziene Katrien Oorts Florian Schneider Maria da Conceição Gonçalves Raquel Mano Gina Garland Rastislav Skalský Lilian O'Sullivan Raimonds Kasparinskis Claire Chenu 《European Journal of Soil Science》2023,74(4):e13398
At the European scale, soil characteristics are needed to evaluate soil quality, soil health and soil-based ecosystem services in the context of the European Green Deal. While some soil databases exist at the European scale, a much larger wealth of data is present in individual European countries, allowing a more detailed soil assessment. There is thus an urgent and crucial need to combine these data at the European scale. In the frame of a large European Joint Programme on agricultural soils launched by the European Commission, a survey was conducted in the spring of 2020, in the 24 European participating countries to assess the existing soil data sources, focusing on agricultural soils. The survey will become a contribution to the European Soil Observatory, launched in December 2020, which aims to collect metadata of soil databases related to all kind of land uses, including forest and urban soils. Based upon a comprehensive questionnaire, 170 soil databases were identified at local, regional and national scales. Soil parameters were divided into five groups: (1) main soil parameters according to the Global Soil Map specifications; (2) other soil chemical parameters; (3) other physical parameters; (4) other pedological parameters; and (5) soil biological features. A classification based on the environmental zones of Europe was used to distinguish the climatic zones. This survey shows that while most of the main pedological and chemical parameters are included in more than 70% of the country soil databases, water content, contamination with organic pollutants, and biological parameters are the least frequently reported parameters. Such differences will have consequences when developing an EU policy on soil health as proposed under the EU soil strategy for 2023 and using the data to derive soil health indicators. Many differences in the methods used in collecting, preparing, and analysing the soils were found, thus requiring harmonization procedures and more cooperation among countries and with the EU to use the data at the European scale. In addition, choosing harmonized and useful interpretation and threshold values for EU soil indicators may be challenging due to the different methods used and the wide variety of soil land-use and climate combinations influencing possible thresholds. The temporal scale of the soil databases reported is also extremely wide, starting from the '20s of the 20th century. 相似文献
127.
Florian Diekmann 《Journal Of Agricultural & Food Information》2013,14(1):14-34
This qualitative study examines data practices of agricultural scientists. A series of individual, semi-structured interviews were conducted with researchers from multiple agricultural disciplines to identify commonalities of data management approaches, to document currently available data management infrastructure, and to explore experiences and attitudes toward sharing of research data. The study highlights the challenges and obstacles agricultural scientists face when managing research data. It shows how barriers to data access and sharing jeopardize usability and longevity of potentially valuable and unique agricultural data sets. 相似文献
128.
Florian Diekmann Robert A. Ford S. Kent Harrison Emilie E. Regnier Ramaro Venkatesh 《Journal Of Agricultural & Food Information》2013,14(4):290-320
Giant ragweed (Ambrosia trifida L.) has become one of the most persistent and problematic weeds to farmers and allergy sufferers in North America over the last decades. This study aims to identify and assess the scholarly literature using a bibliometric analysis approach. Based on bibliographic records retrieved from the Web of Science database, the study describes the periodic growth of literature, scientific fields and journals, author productivity and collaborations, institutions and countries, and topical focus and uses article citation analysis to identify the most influential works in this research field during the period of 1903 to 2012. 相似文献
129.
Edgardo M. Latrubesse Fernando M. d'Horta Camila C. Ribas Florian Wittmann Jansen Zuanon Edward Park Thomas Dunne Eugenio Y. Arima Paul A. Baker 《水产资源保护:海洋与淡水生态系统》2021,31(12):1136-1149
- The extent and intensity of impacts of multiple new dams in the Amazon basin on specific biological groups are potentially large, but still uncertain and need to be better understood.
- It is known that river disruption and regulation by dams may affect sediment supplies, river channel migration, floodplain dynamics, and, as a major adverse consequence, are likely to decrease or even suppress ecological connectivity among populations of aquatic organisms and organisms dependent upon seasonally flooded environments.
- This article complements our previous results by assessing the relationships between dams, our Dam Environmental Vulnerability Index (DEVI), and the biotic environments threatened by the effects of dams. Because of the cartographic representation of DEVI, it is a useful tool to compare the potential hydrophysical impacts of proposed dams in the Amazon basin with the spatial distribution of biological diversity. As the impact of Amazonian dams on the biota of both rivers and periodically flooded riparian environments is severe, DEVIs from different Amazonian tributary basins are contrasted with patterns of diversity and distribution of fish, flooded forest trees and bird species.
- There is a consistent relationship between higher DEVI values and the patterns of higher species richness and endemism in all three biological groups. An assessment of vulnerability at the scale of tributary basins, the assessment of biodiversity patterns related to DEVI, and the analysis of teleconnections at basin scale, demonstrate that recent construction of dams is affecting the biota of the Amazon basin.
- The evidence presented here predicts that, if currently planned dams are built without considering the balance between energy production and environmental conservation, their cumulative effects will increase drastically and represent a major threat to Amazonian biodiversity.
130.
Therese M. Work Pete Weingartner Hugh J. Murphy Ronald Knight Business Lind Sanford Linda Best Florian I. Lauer Joe Pavek Shelley Jansky James Bryan M. D. Groskopp Charles R. Brown Richard G. Clarke John Guerard Jim Davis Rikki Sterrett John Ojala Joseph Guenthner 《American Journal of Potato Research》1989,66(11):769-790