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Measurements were made of the extent of sole and white line lesions on the claws of 115 Holstein-Friesian cows on at least three and at most 16 occasions, and some cows were followed up to their third lactation. All the measurements were made between 12 weeks before calving and 45 weeks after calving. In total, 1016 repeated observations were made. Correlations were calculated between pairs of claws, between types of lesion (sole and white line), and between pairs of the different measurements (number of lesions, proportion of the claw affected, maximum severity score and proportion of the claw affected weighted for severity). The outer hind claws had the greatest extent of lesions of both types. Spearman correlation coefficients and confidence intervals measured the strength of the association. All the associations between claws were positive, suggesting that the lesions did not occur in isolation. Sole and white line lesions were not associated at individual observation points. Lesions on the left and right claws were markedly similar, except for sole lesions on the two inner hind claws, and for white line lesions on the two outer hind claws. 相似文献
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Sections of the medulla oblongata from the brains of sheep were examined for prion protein (PrP) by immunohistochemistry. On the basis of the morphology and neuroanatomical distribution of the deposits, distinct disease-associated patterns of PrP deposition were identified in scrapie-affected sheep, suggesting at least four distinct phenotypes of scrapie. In addition, clearly defined patterns of PrP deposition, readily distinguished from the disease-associated PrP deposits, were identified in some normal sheep from scrapie-free flocks. In five sheep, believed to be preclinically affected by scrapie, PrP deposition of a disease-specific type but of restricted distribution was identified, demonstrating the sensitivity of the technique for the diagnosis of scrapie. The neuroanatomical distribution of these early PrP deposits suggest that the route of entry of the scrapie agent into the brain is via parasympathetic motor neurons in the vagus nerve which innervate the gastrointestinal tract. 相似文献
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