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931.
BACKGROUND: The Marin strain of Culex pipiens Say is a pyrethroid‐resistant population that was collected in Marin County, California, in 2001 and subsequently maintained in the laboratory under regular permethrin exposure. RESULTS: In this study, two cDNAs, CpGSTd1 and CpGSTd2, encoding glutathione S‐transferase (GST) were cloned from Cx. pipiens Marin. Phylogenetic analysis of the deduced amino acid sequences, CpGSTD1 and CpGSTD2, of these genes indicated that they belong to the Delta class of insect GSTs. The nucleotide and deduced amino acid sequences of CpGSTd1 and CpGSTd2 were 59 and 48% identical respectively. CpGSTD1 and CpGSTD2 were expressed in Escherichia coli and purified by affinity chromatography. The recombinant GSTs exhibited unique selectivity towards the general GST substrates 1‐chloro‐2,4‐dinitrobenzene (CDNB) and 1,2‐dichloro‐4‐nitrobenzene (DCNB), and also differed in their sensitivity to known inhibitors of GSTs. CpGSTD1 exhibited peroxidase activity with cumene hydroperoxide, while CpGSTD2 appeared to lack this activity. CpGSTD1 was able to metabolize 1,1,1‐trichloro‐2,2‐bis(4‐chlorophenyl)ethane (DDT), while DDT metabolism by CpGSTD2 was not detectable. CpGSTD1 and CpGSTD2 showed no detectable metabolism of permethrin. Gene expression of CpGSTd1 and CpGSTd2 in Marin mosquitoes was elevated about twofold in comparison with that found in a pyrethroid‐sensitive mosquito strain. CONCLUSION: The results indicate that CpGSTD1 and CpGSTD2 have unique biochemical characteristics, but they do not appear to play major roles in permethrin resistance in Marin mosquitoes. Copyright © 2012 Society of Chemical Industry 相似文献
932.
Purpose
Carbon nanomaterials (CNMs) have attracted a great deal of research interest for their potential environmental applications because of their unique properties. Adsorption of organic chemicals on CNMs was reported to be important in controlling their environmental risks. However, the kinetics of the adsorption is hardly investigated in literature. The objective of this work was, therefore, to quantitatively describe the sorption kinetics of 17 α-ethinyl estradiol (EE2) and bisphenol A (BPA) on CNMs as compared to activated carbon (AC). 相似文献933.
Ruijuan Li Jinfeng Zhao Chuanfan Sun Wenjing Lu Chengjin Guo Kai Xiao 《Frontiers of Agriculture in China》2010,4(2):195-209
As a kind of enzyme widely existing in eukaryotic species, especially in grains and oil seeds, phytases play an important
role in the degradation of some phosphates containing organic molecules. So far, phytases derived from various species have
been successfully used as animal feed additives. It has also been experimentally verified that phytases have a potential use
in generating crop germplasm with high phosphorus use efficiency, based on their biochemical role in releasing Pi from the
phytate and its derivatives. In this paper, the biochemical properties, molecular characterizations, functions and the potential
application perspective of phytases are reviewed and commented on, aiming at the further exploration of the biochemical and
molecular characterizations, and promotion of the application of phytases, a kind of important enzyme possessing potential
use in animal feeding and creation of high P use crop cultivars, in the future. 相似文献
934.
I. Placha M. Pogany Simonova K. Cobanova A. Laukova S. Faix 《Research in veterinary science》2010,89(2):257-261
We investigated the effect of Enterococcus faecium on phagocytic activity, antioxidative status in vivo and the effect of E. faecium and 0.4% concentration of Thymus vulgaris essential oil (EO) on the duodenal tissue integrity in vitro in laying hens. The birds were fed the same standard diets and were divided into four groups. E. faecium was added to the drinking water for the second and fourth groups. EO was added to special chambers for measuring trans-epithelial electrical resistance (TEER) for the third and fourth groups only. TEER was lower in groups where EO was added, but in the group with E. faecium TEER was not changed significantly. Our results show that EO at 0.4% concentration may negatively affect intestine integrity, and the probiotic strain E. faecium AL41 is able to eliminate this effect and can strengthen non-specific immunity. To confirm our findings further histopathological investigations of intestinal tissue are needed. 相似文献
935.
936.
Xing-Pu Li Su-Que Lan Ye-Lun Zhang Yu-Ping Liu 《Genetic Resources and Crop Evolution》2010,57(7):1007-1012
Pericarp color in wheat is an important trait related to nutrients and health benefits. Identification of molecular markers
closely linked to the grain color genes will help utilize pericarp trait in wheat breeding. Genetic studies involving F1, F2, and F3 populations from the cross Jizi 439 (purple grain)/Gao8901 (white grain) showed that the purple grain color was controlled
by two genes. By using the bulked segregant analysis (BSA) approach, two DNA pools were established from the dark purple-grain
and white-grain plants respectively. Among the 230 pairs of microsatellite primers used in this study, two markers showed
polymorphism in DNA pools, parents, F1 and F2 populations. Linkage analysis indicated that the two markers Xgwm47 and Xgwm155 were linked with two purple pigment genes. One of the purple pigment genes is located on chromosome 2AS and is 34.7cM away
from the marker Xgwm47, whereas another gene is mapped on 3AL with 14.7cM away from marker Xgwm155. 相似文献
937.
938.
939.
This study focused on a suite of vascular plant species (six herbs and two grasses) common to traditionally managed, species-rich grasslands in Western Norway. We assessed the suitability of two species transfer methods (seed sowing and soil seed bank) for restoration of species-rich grassland on a newly established road verge. We compared the species' frequencies one and three years after they were sown on a naked, newly created road verge with their frequencies in aboveground vegetation and soil seed banks of comparable, local grasslands. Species frequencies in the aboveground vegetation differed significantly from those in the seed banks. Moreover, the frequencies in the seed banks differed from those recorded one year after sowing, and the frequencies in the aboveground vegetation differed from those recorded three years after sowing. Avenula pubescens and Knautia arvensis, found in more than 25% of the aboveground grassland plots, did not germinate from any of the seed bank samples. Festuca rubra, Galium verum, Pimpinella saxifraga and Silene vulgaris were more frequent in the aboveground plots than in the seed bank samples. Pimpinella saxifraga, Galium verum and Lychnis viscaria emerged quite well both from sown seeds and from the seed bank. Avenula pubescens was frequent in the aboveground vegetation, but did not germinate from sown seeds. Six species established well from seeds, and most increased in frequency in the sown plots from the first to the third year. No species was found in the sown plots only, but three years after sowing, three species were more frequent in the sown plots than in the aboveground vegetation of donor grassland plots. Our fine-scale, point-to-point study demonstrates that different restoration methods produce widely differing species composition even when the donor material is identical. We propose that different substrates and a combination of establishment methods (sowing and hay transfer) are needed as supplements to seed banks to re-establish species-rich grassland. 相似文献
940.
Living in poverty is associated with high levels of protracted stress associated with health problems. Economic and food insecurity
are particularly poignant aspects of poverty and condition the work of securing basic daily needs of families. Recent studies
suggest that levels of stress increase as family food needs rise. This paper presents new findings which clarify the relationship
of food provisioning to stress levels, by examining actual food provisioning strategies and food insecurity among the Northern
Cheyenne Indians of southeastern Montana. Results clearly show that stress varies by types of food acquisition strategies.
Contrary to our expectations, more complex strategies, including relatively unpredictable and cumbersome food provisioning
activities, are not linked to higher stress levels in our analysis. Controlling for food security levels, households using
a combination of local programs and informal subsistence sources are the least stressed, despite the demands of managing a
large number of food sources. Households primarily using Food Stamps are the most likely to experience high levels of stress.
Interviews with Food Stamp recipients show that potential sources of stress include inadequate allocations of Food Stamps,
difficulty achieving and maintaining eligibility, challenges to complying with paperwork and appointment requirements, as
well as personal obstacles and community barriers to making food stamps last. Analyses indicate that contradictions between
local cultural norms for food provisioning and the realities of food insecurity in this context promote strategies emphasizing
greater independence from federal food programs. 相似文献