竹子基因调查分析报告

竹子作为一种重要经济植物,具有经济、生态和文化价值。随着生物技术的快速发展,越来越多的以竹类植物为对象的基因序列得到克隆与验证,基于BPG(2012)更新系统,截至2014年底,已经有3913个竹子基因得到确证,这些基因覆盖了竹亚科的3个族,隶属19个属30个种。文章统计分析了已确认的基因描述、基因类型和参考序列情况,展望了竹子基因发展态势。     

城市森林生态服务居民自愿供给分析

城市森林生态服务的多重属性导致市场和政府供给均存在失灵问题。文中沿循公共选择理论和行为经济学、实验经济学社会偏好理论体系, 构建了一个基于居民自愿捐献行为的城市森林生态服务供给逻辑, 据此提出城市森林生态服务居民自愿供给的社区模式、俱乐部模式和公益平台模式, 并给出政策建议。     

Establishment of a high-frequency regeneration system in <Emphasis Type="Italic">Cerasus humilis</Emphasis>, an important economic shrub

Cerasus humilis is a species of small, perennial, drought-resistant and multipurpose deciduous shrub grown in arid and semi-arid conditions in northern China. In this study, an efficient protocol for the rapid micropropagation of C. humilis has been standardized using stem and/or leaf explants. Direct multiple shoot induction was observed when the stem explants were cultured on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. The highest shoot induction was obtained when stem explants from adult trees were cultured on MS medium supplemented with 2.0 mg L^?1 6-benzyladenine (6-BA) and 0.9 mg L^?1 α-naphthaleneacetic acid (NAA). The leaf and stem explants cultured on MS medium with 1.0 mg L^?1 6-BA and 0.6 mg L^?1 NAA, and 0.5 mg L^?1 6-BA and 0.8 mg L^?1 NAA, respectively, produced the highest induction frequency of callus. Maximum proliferation of callus was observed on MS medium containing a combination of 0.5 mg L^?1 6-BA with 0.6 mg L^?1 2,4-dichlorophenoxyacetic acid (2,4-d). Optimal shoots differentiated from callus were obtained on MS medium supplemented with 5.0mg L^?1 6-BA and 0.9 mg L^?1 NAA. In vitro rooting was achieved on half-strength (1/2) MS medium containing 0.5 mg L^?1 NAA. Rooted plantlets were hardened under control conditions and successfully acclimatized under field conditions.     

BL-甲醛消纳剂在环保细木工板生产中的应用研究

从细木工板的发展现状概述了开发绿色环保细木工板的必要性,介绍了试制绿色细木工板的试验结果。试验结果显示,BL-甲醛消纳剂与UF树脂兼容,加消纳剂后的UF树脂的黏度及pH可满足生产需要。在UF树脂中加入10%左右的消纳剂在实验室及试生产中可生产出物理力学性能合格的E_0细木工板。     

如何走出一条解困发展的路子 --关于内蒙古五岔沟、阿尔山林业局的调查

内蒙古五岔沟林业局和阿尔山林业局5年实现林业跨越式发展,创新机制是其发展的根本经验。两个林业局在机制转变、改革创新、发展非公有制、加强科学管理等方面提供了借鉴。通过调研针对改革实践中存在的问题提出了政策建议。     

Assessment of Rhizospheric Microorganisms of Transgenic Populus tomentosa with Cowpea Trypsin Inhibitor (CpTI) Gene

To have a preliminary insight into biosafety of genetically transformed hybrid triploid poplars (Populus tomentosa × P. bolleana) × P. tomentosa with the cowpea trypsin inhibitor (CpTI) gene, two layers of rhizospheric soil (from 0 to 20 cm deep and from 20 to 40 cm deep, respectively) were collected for microorganism culture, counting assay and PCR analysis to assess the poten-tial impact of transgenic poplars on non-target microorganism population and transgene dispersal. When the same soil layer of suspension stock solution was diluted at both 1:1 000 and 1:10 000 rates, there were no significant differences in bacterium colony numbers between the inoculation plates of both transgenic and non-transgenic poplars. The uniform results were revealed for both soil layer suspension solutions of identical poplars at both dilution rates except for non-transgenic poplars at 1:10 000 dilution rates from the same type of soil. No significant variation in morphology of both Gram-positive and Gram-negative bacteria was observed under the microscope. The potential transgene dispersal from root exudates or fallen leaves to non-target microbes was repudiated by PCR analysis, in which no CpTI gene specific DNA band was amplified for 15 sites of transgenic rhizospheric soil samples. It can be concluded that transgenic poplar with the CpTI gene has no severe impact on rhizospheric microorganisms and is tentatively safe to surrounding soil micro-ecosystem.     

电磁屏蔽功能胶合板初探

为了赋予木基复合材料电磁屏蔽功能,在脲醛树脂胶中加入碳、石墨等导电单元,制备3层结构的杨木胶合板,研究不同导电单元对胶合板电磁屏蔽效能的影响.结果表明:导电单元的粒度越小,胶合板的电磁屏蔽效能越好,最大可达22 dB,但相关机理和量化关系还有待于深入研究.     

城市道路改造交通设计导则研究

通过对南京市主干道改造前交通特性分析,研究确定该道路改造应遵循的交通设计原则和合理的交通组织方案。旨在探讨城市道路改造过程中如何运用现代城市交通设计理念,实现支撑土地开发利用、提高道路交通效率的交通设计思想和导则。对于我国其他城市开展此类项目可起到一定的参考价值。     

FSS系列桥梁伸缩缝的计算机仿真分析

采用ANSYS通用分析软件,对FSS系列桥梁伸缩缝进行了计算机仿真分析,并详细介绍了用AN-SYS仿真分析的过程。对伸缩缝锚固装置及伸缩缝与加强混凝土间的受力及抗裂性能进行分析,通过与试验值的比较,验证了仿真结果的真实、可靠性。     

纤维素酶催化水解和氧化机制的研究进展

人类对纤维素的开发与利用还非常有限,未能完全搞清天然纤维素的生物降解机制是其一个重要原因.自从C1-Cx假说提出以来,又有较多的降解理论出现对其改进,纤维素酶水解机制不能完全解释天然纤维素的降解,大量文献证实天然纤维素降解存在氧化机制.本文主要介绍水解与氧化机制中具代表性酶协同降解模型、纤维二糖水解酶(CBH)协同作用假说、羟基自由基的氧化机制和纤维二糖脱氢酶途径等不同降解理论,将水解和氧化两种机制结合起来给出其降解途径,也许能够较为容易理解纤维素的降解具体过程.