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91.
Tatiene R. M. Silva Flvia N. L. Barros Michele Bahia Francisco D. Sampaio Junior Sidney S. F. Santos Larissa S. Inoue Thamirys S. Gonalves Laerzio Chiesorin Neto Diogo C. L. O. Faria Camila Tochetto Gisele M. R. Viana Frederico O. B. Monteiro Gustavo Ges-Cavalcante Alessandra Scofield 《Zoonoses and public health》2019,66(7):798-804
The Brazilian Amazon is endemic for malaria and natural infections by Plasmodium spp. have been detected in Neotropical primates. Despite the diversity of primate species in the region, studies on infections by these agents are limited. The aim of the present study was to investigate the frequency of infection by Plasmodium vivax and P. falciparum in free‐born primates that were kept in captivity, in the western Amazon, Brazil. Blood samples were collected from 98 Neotropical primates. Detection of P. vivax and P. falciparum DNA was performed using a semi‐nested PCR, and the amplified products were sequenced. Plasmodium spp. DNA was detected in 6.12% (6/98) of the primates. P. vivax, and P. falciparum DNA was detected in 2.04% (2/98) and 4.08% (4/98) of these mammals, respectively. Sequencing and phylogenetic analysis confirmed the results obtained from the semi‐nested PCR. The presence of infected non‐human primates (NHP) can be auxiliary in the maintenance of P. falciparum and P. vivax and may have implications for the malaria surveillance and control in the Brazilian Amazon. It is necessary to structure an efficient surveillance system for the aetiological agents of malaria that infect NHP and humans to reduce the risk of Plasmodium spp. introduction into new areas, to protect all susceptible species. 相似文献
92.
Raquel Lunedo Luiz R. Furlan Miguel F. Fernandez‐Alarcon Gustavo H. Squassoni Daniel M. B. Campos Dani Perondi Marcos Macari 《Journal of animal physiology and animal nutrition》2019,103(4):1070-1080
The present study was conducted to verify how feed restriction affects gut microbiota and gene hepatic expression in broiler chickens and how these variables are related to body weight gain. For the experiment, 21‐d‐old Cobb500TM birds were distributed in a completely randomized experimental design with three treatments: T1. Control (ad libitum—3.176 Mcal/kg ME—metabolizable energy—and 19% CP—crude protein); T2. Energetic restriction (2.224 Mcal/kg ME and 19% CP) from 22 to 42 days with consumption equivalent to control; T3. Quantitative restriction (70% restriction, i.e., restricted broilers ingested only 30% of the quantity consumed by the control group—3.176 Mcal/kg ME and 19% CP) for 7 days, followed by refeeding ad libitum from 28 to 42 days. Ileum and caecum microbiota collections were made at 21, 28 and 42 days of age. Hepatic tissue was collected at 28 and 42 days old for relative gene expression analyses. At 43‐d‐old, body composition was quantified by DXA (Dual‐energy X‐ray Absorptiometry). Both feed restriction programmes decreased Lactobacillus and increased Enterococcus and Enterobacteriaceae counts. No differences were found in the refeeding period. Energetic restriction induced the expression of CPT1‐A (Carnitine palmitoyltransferase 1A) gene, and decreased body fat mass. Quantitative feed restriction increased lipogenic and decreased lipolytic gene expression. In the refeeding period, CPT1‐A gene expression was induced, without changing the broilers body composition. Positive associations were found between BWG (Body Weight Gain) and Lactobacillus and Clostridium cluster IV groups, and negatively associations with Enterobacteriaceae and Enterococcus bacterial groups. In conclusion, differences found in microbiota were similar between the two feed restriction programmes, however, hepatic gene expression differences were only found in quantitative restriction. Higher counts of Lactobacillus and Clostridium cluster IV groups in ileum are likely to be related to better broiler performance and low expression of lipogenic genes. 相似文献
93.
Cardoso Daniel Barros Véras Robson Magno Liberal de Carvalho Francisco Fernando Ramos Magalhães André Luiz Rodrigues de Vasconcelos Gustavo Araújo Maciel Maria Inês Sucupira Madruga Marta Suely Urbano Stela Antas de Lima Silva Janaina 《Tropical animal health and production》2019,51(6):1515-1521
Tropical Animal Health and Production - The objective of this study was to evaluate the effect of the replacement of corn by cassava dregs (0, 33, 66, and 100%) in the diet of lamb on... 相似文献
94.
Balaro Mario Felipe A. de Mello Samuel Guaraná Valverde da Silva Santos Alex Cavalcanti Luiza Mattos Almosny Nádia Regina Pereira Fonseca Jeferson F. Brandão Felipe Z. 《Tropical animal health and production》2019,51(2):345-353
Tropical Animal Health and Production - This study aimed to verify the reproductive seasonality in Saanen goats from distinct parity orders (nulliparous, primiparous, and pluriparous) throughout an... 相似文献
95.
Sonia Jurado José A Orden Pilar Horcajo Ricardo De La Fuente José A Ruiz-Santa-Quiteria Susana Martínez-Pulgarín Gustavo Domínguez-Bernal 《Journal of veterinary diagnostic investigation》2008,20(3):342-345
Thirty-seven fluoroquinolone-resistant Escherichia coli strains from ruminants (according to Clinical and Laboratory Standards Institute guidelines) were screened by molecular methods for mutations in the quinolone resistance-determining region (QRDR) of the gyrA and parC genes and for the presence of the qnrA gene. One of the strains studied was an enterohemorrhagic E. coli (EHEC) strain potentially pathogenic for humans. Three E. coli strains resistant to enrofloxacin (minimal inhibitory concentration [MIC] = 2 microg/ml) but not to ciprofloxacin (MIC = 1 microg/ml) presented single mutations in the gyrA and parC genes, while 34 strains resistant to both fluoroquinolones presented double and single mutations in gyrA and parC, respectively (31 strains), or double mutations in gyrA and parC (3 strains). The EHEC strain presented a double amino acid substitution in the GyrA protein (Ser-83-->Leu and Asp-87-->Gly) and a double amino acid substitution in the ParC protein (Gly-78-->Cys and Ser-80-->Arg), one of which has not been previously described. The present study shows that most of the mutations in the QRDR of the gyrA and parC genes of fluoroquinolone-resistant E. coli strains from ruminants are the same as those seen in E. coli strains from other animal species and humans and that there are no differences in mutation patterns in the QRDR of E. coli strains from healthy ruminants and those with diarrhea. No strains carried qnrA, which indicates that this gene does not play an important role in the selection of fluoroquinolone-resistant E. coli strains from ruminants. 相似文献
96.
Gabriela Noronha de Toledo Pamela Rodrigues Reina Moreira Daniele Santos Rolemberg Andréia Coutinho Facin Julio Edward Hough Monteiro Mareliza Possa de Menezes Maria Eduarda Bastos Andrade Moutinho da Conceição Felipe Augusto Ruiz Sueiro Rosemeri de Oliveira Vasconcelos Paola de Castro Moraes Mirela Tinucci Costa Andrigo Barboza de Nardi 《Topics in companion animal medicine》2018,33(2):35-39
Osteosarcoma is the most commonly reported bone tumor in dogs, typically affecting the axial and appendicular skeleton. Involvement of the paranasal sinuses and the nasal cavity is extremely rare. This report describes a 6-year-old, female, cross-breed dog presented with signs of 90 days duration and diagnosed with a mass located in the right nasal cavity. Radiography, rhinoscopy, and computed tomography findings suggested the presence of an intranasal tumor. Osteosarcoma in the nasal cavity was diagnosed based on histopathological examination and clinical and imaging findings. Survival time was 12 months from diagnosis. 相似文献
97.
Characterization of the LH peak after short and long fixed‐time artificial insemination protocols in sheep raised in the tropics
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Gabriela Azenha Milani Soriano Felipe Rydygier de Ruediger Marilice Zundt Marco Aurélio da Cruz Nobre Gomes Lilian Francisco Arantes de Souza Ines Cristina Giometti Guilherme de Paula Nogueira Gustavo Ricci Zanelli Fabiola Cristiane de Almeida Rego Caliê Castilho 《Animal Science Journal》2018,89(9):1245-1252
The aim of this study was to evaluate the peak in luteinizing hormone (LH) and the pregnancy rate of sheep (Texel × Santa Inês) in the tropics using short‐ (6 days) and long‐term (12 days) progesterone protocols followed by artificial insemination (AI) both in and out of the breeding season. Experiment 1 was conducted within (IN) the breeding season (autumn, n = 36), and experiment 2 was conducted outside (OUT) of the breeding season (spring, n = 43). In each experiment, the sheep were divided into two groups (6 or 12 days) according to the duration of treatment with a single‐use progesterone release vaginal device (CIDR®, Pfizer, São Paulo, SP, Brazil), and blood samples were collected from 10 animals per group every 4 hr to measure the LH and progesterone concentrations. In the spring, the characteristics of the LH peak did not differ between groups; but in the autumn, there were differences between groups at the beginning (G‐6 IN: 36.44 ± 5.46 hr; G‐12 IN: 26.57 ± 4.99 hr) and end of the LH peak (G‐6 IN: 46.22 ± 7.51 hr; G‐12 IN: 34.86 ± 8.86 hr). The results showed alterations in the LH peak during the breeding season only in the sheep undergoing the short‐term protocol. 相似文献
98.
Garcia JL Navarro IT Biazzono L Freire RL da Silva Guimarães Junior J Cryssafidis AL Bugni FM da Cunha IA Hamada FN Dias RC 《Veterinary parasitology》2007,145(3-4):197-206
This study evaluated a vaccine made from crude rhoptry proteins of Toxoplasma gondii with Quil-A, which was administered to cats by the intranasal route. Eleven short-hair domestic cats were divided into four groups: G1 (n=3) received three doses (200 microg/dose) of the rhoptry vaccine with Quil-A (20 microg); G2 (n=3) received PBS with Quil-A (20 microg); G3 (n=3) and G4 (n=2) received only PBS. Treatments were administered at days 0, 21, and 42 by the intranasal route. Challenge was done to G1, G2, and G3 animals with 600 cysts of the VEG strain on day 51 (challenge day); G4 animals were unchallenged. The anti-T. gondii IgG and IgA antibody levels from sera were measured by indirect enzyme-linked immunosorbent assay (ELISA). At challenge, two animals from G1 revealed antibody levels for both IgG and IgA; oocysts were not detected in feces of these two cats. There were no differences in hematological values between groups throughout the experiment (p>0.10). Preventable fractions were 67% in G1 and 0% in G2 and G3. Comparatively, G1 animals shed 89.3% and 90.8% less oocysts than G3 and G4, respectively. Two out of three cats were protected against T. gondii oocyst shedding when the rhoptry vaccine was administered by the intranasal route. This is the first study using crude rhoptry proteins as vaccine by the intranasal route in cats to evaluate protection against oocysts shedding. 相似文献
99.
Buentello JA Reyes-Becerril M Romero-Geraldo Mde J Ascencio-Valle Fde J 《Journal of aquatic animal health》2007,19(3):195-203
The effects of elevated dietary arginine on the hematology and immune function of juvenile channel catfish Ictalurus punctatus were evaluated by means of in vivo and in vitro experiments. Healthy juvenile channel catfish (average weight, 34.8 g) were fed casein-gelatin-based diets containing 28% crude protein and supplemented with crystalline L-arginine (ARG) at 0.5, 1, 2, or 4% of diet. An intact-protein diet containing 1.3% arginine also was included to investigate the effects of amino acid form (crystalline-free amino acids versus intact protein). Each purified diet was fed to apparent satiation to triplicate groups of fish for 6 weeks. At the end of the experimental feeding period, the fish were injected intraperitoneally with two doses (3 d apart) of 2 mg lipopolysaccharide/kg body weight. Six days after the initial injection, the fish were anesthetized and tissue samples were obtained to evaluate hematological and humoral and cellular immune parameters, including phagocytic activity of peritoneal macrophages, hemoglobin, hematocrit, mean corpuscular volume (MCV), blood cell counts, plasma protein, and hepatic superoxide dismutase activity. High dietary levels (4% ARG) resulted in significantly higher levels of hemoglobin, hematocrit, and circulating erythrocytes. Dietary ARG did not significantly affect MCV and the number of circulating leukocytes, lymphocytes, eosinophils, and monocytes. In vitro, a moderate level (2 mM) of ARG in the culture media was found to be ideal in significantly enhancing phagocytosis. This study demonstrates that some aspects of the immune system of channel catfish are sensitive to changes in dietary ARG. 相似文献
100.
The effect of plant breeding on yield and their physiological determinants has been widely studied in wheat. However, it is
poorly understood how, and to what extent, yield stability has been modified. To attempt a direct analysis of changes in absolute
and relative yield stability, data of yield of cultivars released in different eras in different environments were obtained
from records from our lab and from the literature. Depending on the availability of data, effects of plant breeding on yield
stability of cultivars released in Argentina, Australia, Italy and the United Kingdom were evaluated using a quantitative
approach. In this paper it was assumed that the slope of yield vs. environmental index estimates the instability of the cultivars.
In addition, a more qualitative approach for Mexico, and the former USSR complemented this analysis. There was a clear decrease
in yield stability assessed in absolute terms as a consequence of wheat breeding. In Argentina, Australia, Italy and the UK
this decrease was related to the magnitude of yield increases. However, the decrease in yield stability in Argentina and Australia
was less than for Italy and the UK, particularly so during the last 30 years. Modern cultivars released in Argentina and Australia
showed a trend to maintain yield stability as a percentage of their yield similar to that of their predecessors, while the
two European countries analysed tended to a slight decrease in yield stability even in relative terms. The complementary,
less quantitative evaluation of Mexico and the former USSR appeared to confirm the quantitative trends described for the other
countries, i.e. a general decrease in yield stability (assessed in absolute terms) with genetic gains in yield potential.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献