Ghrelin对雌激素诱导小鼠胸腺萎缩过程中细胞因子及凋亡相关蛋白基因表达的影响

应用光镜和实时定量PCR技术研究了Ghrelin对雌激素诱导小鼠胸腺萎缩过程中胸腺形态学以及部分细胞因子和凋亡相关蛋白基因表达的影响。结果显示,注射Ghrelin后,雌激素诱导的小鼠萎缩胸腺在形态学上基本恢复到正常水平,胸腺中IL-6、TGF-[31、Caspase3、FADDmRNA含量显著降低,Caspase9、FasLmRNA含量略为下降,而IL-7、Bcl-2mRNA含量有所上升。结果表明,Ghrelin可能通过促进胸腺上皮细胞的增殖以及阻断Caspase级联程序和Fas／FasL凋亡信号通路抑制胸腺细胞的凋亡,从而逆转雌激素诱导的小鼠胸腺萎缩。     

Pharmacokinetics of mequindox and one of its major metabolites in chickens after intravenous, intramuscular and oral administration

Pharmacokinetics of mequindox and one of its major metabolites (M) was determined in chickens after intravenous (i.v.), intramuscular (i.m.) and oral administration of mequindox at a single dose of 10 (i.v. and i.m.) or 20 mg/kg b.w. (oral). Plasma concentration profiles were analyzed by a non-compartmental pharmacokinetic method. Following i.v., i.m. and oral administration, the areas under the plasma concentration-time curve (AUC(0-∞)) were 0.71±0.15, 0.67±0.21, 0.25±0.10 μg h/mL (mequindox) and 37.24±7.98, 36.40±9.16, 86.39±16.01 μg h/mL (M), respectively. The terminal elimination half-lives (t(1/2λz)) were determined to be 0.15±0.06, 0.21±0.09, 0.49±0.23 h (mequindox) and 5.36±0.86, 5.39±0.52, 5.22±0.35 h (M), respectively. The bioavailabilities (F) of mequindox were 89.4% and 16.6% for i.m. and oral administration. Steady-state distribution volume (V(ss)) of 1.20±0.34 L/kg and total body clearance (Cl(B)) of 13.57±2.16 L/kg h were determined for mequindox after i.v. dosing. After single i.m. and oral administration, peak plasma concentrations (C(max)) of 3.04±1.32, 0.36±0.13 μg/mL (mequindox) and 3.81±0.92, 5.99±1.16 μg/mL (M) were observed at t(max) of 0.08±0.02, 0.32±0.12 h (mequindox) and 0.66±0.19, 6.67±1.03 h (M), respectively. The results showed that mequindox was rapidly absorbed after i.m. or p.o. administration and most of mequindox was transformed to metabolites in chickens, with much higher C(max)s and AUCs of metabolite (M) than those of mequindox in plasma.     

早期妊娠猕猴孕激素水平的检测

利用全自动化学发光免疫分析仪检测正常未妊娠的与不同孕期猕猴血清中雌二醇及孕酮的分泌水平。结果表明孕5d、10d、25d、30d血清孕酮比正常猕猴的分泌水平有非常明显的上升（P〈0.01）,孕15d也有显著的上升（P〈0.05）;孕15d、20d的雌二醇分泌水平比正常组有非常显著升高（P〈0.01）,而孕5d、10d、2...     

The role of bisphosphonates in the management of patients that have cancer.

Bisphosphonates are pharmacologic agents widely used in people for managing pathologic bone resorptive conditions. Based on their physicochemical properties, bisphosphonates concentrate within areas of active bone remodeling and induce osteoclast apoptosis. Appropriate use of bisphosphonates for treating companion animals requires a thorough understanding of how bisphosphonates exert their biologic effects. This review article highlights general properties of bisphosphonates, including their pharmacology, mechanisms of action, adverse side effects, anticancer mechanisms, surrogate markers for assessing response, and potential clinical utility for treating dogs and cats diagnosed with malignant skeletal tumors.     

辽西地区野兔危害情况调查

通过走访、问卷调查和样地调查,对辽西地区野兔危害情况进行了初步调查,并对调查数据进行了统计分析。结果表明：辽西地区野兔主要危害杏树,其次是刺槐、杨树和梨树等;野兔对不同林龄杏树危害差异显著。其中,对3林龄以下杏树危害严重,3林龄以上危害较轻;野兔对不同地区杏树危害程度差异显著,主要表现在对海拔较高降雨较少的地区危害程度较海拔低降雨相对多的地区高。     

动物疫病区域化管理的新模式——生物安全隔离区划

2003年,OIE提出了动物疫病区域化政策的新理念——生物安全隔离区划(Compartmentalisation),即通过采取生物安全管理控制措施等手段,建设基于企业水平的无疫生物安全隔离区。从2004年起,OIE先后在《陆生动物卫生法典》和《OIE公告》中提出了定义、评价和确认生物安全隔离区的原则、要素和步骤建议,为世界各国开展生物安全隔离区建设评估工作提供了方向性指导。虽然生物安全隔离区划的概念提出时间较短,但作为一种全新的动物疫病区域化管理模式,生物安全隔离区划已经引起了世界范围的广泛关注,美国、泰国等畜牧业发达国家均在积极研究探索生物安全隔离区建设评估的模式。     

ELISE测定猪尿、猪肝中克伦特罗残留的研究

ELISA(酶联免疫吸附法)测定猪尿、猪肝中违禁药物克伦特罗残留量影响实验结果表明,德国拜发与北京望尔生产的两种ELISA试剂盒具有操作简单、时间短、成本低、敏感度高等特点。在室温20-22℃条件下,试验制备过程中必须做到控温度、控转速、控流速及洗板过程中应注意事项,这样可大大提高在实际操作过程中检测的准确性。     

猪繁殖与呼吸综合征病毒致病机理的研究进展

猪繁殖与呼吸综合征(PRRS)是由猪繁殖与呼吸综合征病毒(PRRSV)引起的一种以妊娠母猪严重繁殖障碍及仔猪的呼吸道症状和高死亡率为特征的传染病。现已遍及世界各主要养猪国家和地区,成为危害养猪业最严重的传染病之一。作者就猪繁殖与呼吸综合征病毒致病机理方面的研究情况作一综述,为诊断技术、免疫机理研究、疫苗设计和疫病防制提供借鉴。     

新城疫病毒实时荧光RT-PCR检测方法的建立与应用

为满足新城疫病毒高通量快速检测的需要,针对新城疫病毒M基因序列,通过基因比对分析保守区域,经同源性分析后,设计合成多条引物和探针并对其筛选,建立了一种能够快速检测新城疫病毒的实时荧光RTPCR检测方法,并对该方法的特异性、灵敏性进行了评估。结果显示,该方法检测耗时短、特异性好,检测下限达10~(-4) ng/μL。利用该方法,对480份临床采集的各类家禽咽拭子样品进行检测,共检测出25份阳性,与常规RT-PCR检测方法结果一致,κ值为1(P 0.001)。结果表明,该方法快速、准确,可用于新城疫病毒的快速检测。