柑橘褐斑病菌对不同抗性柑橘品种防御酶活性的影响

以柑橘褐斑病高抗品种Lw8,中抗品种L2,高感品种Lw14为实验材料,通过孢子喷雾法使柑橘褐斑病菌感染柑橘叶片,研究接菌0d、1d、2d、3d、6d、8d、10d时,柑橘叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、几丁质酶(CHT)酶和β-1,3葡聚糖酶(GLU)含量及活性变化规律与品种抗病性的关系。结果表明,接种前,两个抗病品种Lw8、L2中的POD、SOD、PAL、PPO 、GLU、CHT活性显著高于高感品种Lw14,CAT活性则与高感品种Lw14相近;接种后,3个不同抗性品种中SOD、POD、CAT、PAL、PPO 、GLU、CHT 7种防御酶活性较对照明显提高,但不同品种增加幅度不一致,除CAT外,其余6种防御酶活性均表现为抗病品种Lw8、L2增幅显著高于高感品种Lw14,且两个抗病品种一般在接菌3d内6种防御酶活性迅速升高并达到峰值;而高感品种Lw14防御酶(除CAT外)活性或增幅较小或较两个抗病品种滞后。本研究初步探讨了不同抗性品种接种褐斑病菌后7种防御酶的活性动态变化与柑橘品种抗病性关系,为进一步研究其抗病机理奠定基础。     

接触二甲苯实验人员淋巴细胞染色体畸变分析

目的:研究二甲苯对实验人员外周血淋巴细胞染色体畸变的影响.方法:外周血培养制备染色体标本,分析外周血淋巴细胞染色体畸变.结果:二甲苯接触组的染色体数目畸变细胞率(11.3%)高于对照组(6.8%)(P＜0.05);结构畸变细胞率(包括裂隙)(3.57%)高于对照组(2.6%)(P＜0.05)并且呈剂量效应关系;接触组的未成熟着丝粒分离细胞率(1.85%)与对照组(1.34%)相比无显著差异(P＞0.05).结论:二甲苯接触导致外周血淋巴细胞染色体畸变增加;二甲苯接触是否诱导未成熟着丝粒分离有待进一步深入研究.     

油菜秸秆饲料化潜力及展望

为了研究油菜秸秆饲料化利用技术,综述了国内外近30年对油菜秸秆的研究,发现油菜秸秆粗脂肪和粗蛋白含量较高,饲用潜力大,但油菜秸秆因其木质化程度高、适口性差在实际生产中难以饲喂而存在大量丢弃、焚烧的情况。有关油菜秸秆饲料化技术的研究还存在不足,油菜秸秆饲用的限制因素难以得到解决,因此,今后还需研究如何改善油菜秸秆的适口性以增加动物的采食量,提高油菜秸秆的利用效率。本研究从油菜秸秆的饲用潜力出发,探讨了限制油菜秸秆饲用的因素,综述了目前已有的氨化、青贮、微贮等油菜秸秆饲料化技术,分析了不同技术的优缺点,并进一步提出了未来油菜秸秆饲料化有可能的研究方向。     

Evaluation on the fitness and population projection of Nilaparvata lugens in response to elevated CO2 using two-sex life table

Abstract

The life history and fitness of Nilaparvata lugens being reared under ambient level (current CO₂ concentration), medially elevated (550?µL/L) and highly elevated (750?µL/L) CO₂ concentration for long-term generation were compared using two-sex life table instead of traditional age-specific life table. The results showed that significantly longer larval duration and lower fecundity of N. lugens were observed in 750?µL/L relative to 550?µL/L treatment (P?<?0.05). Accordingly, 550?µL/L CO₂ significantly enhanced population parameters of N. lugens, including significantly higher intrinsic rate of increase (r), finite rate (λ) and net reproductive rate (R_O), but not for 750?µL/L CO₂. Taken together, N. lugens performs enhanced development rate, fecundity and survival in individual life history and higher potential in population multiplication under 550?µL/L CO₂ level, while only indicates the less enhanced development rate and survival without significant increased capacity of population expansion under 750?µL/L CO₂ level. These results should facilitate predicting the fitness and potential population damage of N. lugens, which is valuable for the integrated control of N. lugens in the future increasing CO₂ concentration.     

Integrating meteorological factors for better understanding of the urban form-air quality relationship

Landscape Ecology - Understanding the urban form-air quality relationship is essential to mitigate intra-urban air pollution and to improve urban ecology. However, few studies considered urban form...     

重组酶聚合酶等温扩增技术在动物源性成分鉴定中的应用

在打击肉制品掺假走私、维护市场秩序、保护野生动物资源等方面,对动物源性成分鉴定技术的特异性、灵敏性、重复性提出了很高的要求。本文阐述了动物源性成分的检测鉴定技术,有多样性和局限性的特点。归纳了重组酶聚合酶恒等温扩增技术原理与应用,解决了PCR技术在仪器上的局限性,优化了早期恒温扩增技术的缺点。指出重组酶聚合酶恒等温扩增技术为动物源性成分的快速准确鉴定提供了坚实的技术基础,并在动物疫病诊断方面也有很好的应用前景。     

大豆miR482家族的生物信息学分析

为进一步对大豆中的miR482家族有更全面的了解,本研究以大豆miR482家族为研究对象,对大豆中miR482家族的前体及成熟序列的特征、靶基因预测及其表达模式进行分析.在通过miRBase搜索得到的gma-miR482家族成熟序列中,除gma-miR482b/e均位于chr.20以外,其余序列均位于不同染色体.gma...     

Generation of a uniform thymic malignant lymphoma model with C57BL/6J p53 gene deficient mice

Lymphoma is the third most common cancer diagnosed in children, and T-cell lymphoma has the worst prognosis based on clinical observations. To date, a lymphoma model with uniform penetrance has not yet been developed. In this study, we generated a p53 deficient mouse model by targeting embryonic stem cells derived from a C57BL/6J mouse strain. Homozygous p53 deficient mice exhibited a higher rate of spontaneous tumorigenesis, with a high spontaneous occurrence rate (93.3%) of malignant lymphoma. Because tumor models with high phenotypic consistency are currently needed, we generated a lymphoma model by a single intraperitoneal injection of 37.5 or 75 mg/kg N-methyl-N-nitrosourea to p53 deficient mice. Lymphoma and retinal degeneration occurred in 100% of p53^+/− mice administered with higher concentrations of N-methyl-N-nitrosourea, a much greater response than those of previously reported models. The main anatomic sites of lymphoma were the thymus, spleen, bone marrow, and lymph nodes. Both induced and spontaneous lymphomas in the thymus and spleen stained positive for CD3 antigen, and flow cytometry detected positive CD4 and/or CD8 cells. Based on our observations and previous data, we hypothesize that mice with a B6 background are prone to lymphomagenesis.