The protein,non-protein and total nitrogen inSolanum tuberosum,ssp.andigena potatoes

Total protein estimated from 80% ethanol insoluble nitrogen in residue ofS. tuberosum, ssp.andigena tubers agreed well with results obtained by Potty method and estimation of non-dialyzable nitrogen. The method is simple, rapid and applicable for screening large numbers of samples. Dry matter, total nitrogen, protein nitrogen and non-protein nitrogen were analyzed for 50 clone samples harvested in Huancayo, Peru, and correlations between the various constituents are statistically analyzed and discussed. An increase in total nitrogen with an increase of protein nitrogen was observed. Clones with higher dry matter normally showed lower % of non-protein nitrogen. The percentage of protein nitrogen to total nitrogen varied from 40 to 74% in the 50 clone samples and clonal differences in protein production were evident.     

数字乡村发展的实践与探索——基于北京的调研

研究旨在通过分析北京市数字乡村建设实践,发现存在问题,找出推进路径,为数字乡村发展提供可借鉴经验.利用文献分析、典型案例和专家咨询法,探讨数字乡村发展理论与实践.数字化是现代农业4.0阶段的关键特征,数字经济是农业农村高质量发展的新引擎、新动能.北京是数字乡村发展的先行者,为北京乡村振兴和农业现代化建设打下了坚实基础....     

Relationship between stable carbon isotope discrimination and water use efficiency under regulated deficit irrigation of pear-jujube tree

To investigate the relationship between stable carbon isotope discrimination (Δ) of different organs and water use efficiency (WUE) under different water deficit levels, severe, moderate and low water deficit levels were treated at bud burst to leafing, flowering to fruit set, fruit growth and fruit maturation stages of field grown pear-jujube tree, and leaf stable carbon isotope discrimination (Δ_L) at different growth stages and fruit stable carbon isotope discrimination (Δ_F) at fruit maturation stage were measured. The results indicated that water deficit had significant effect on Δ_L at different growth stages and Δ_F at fruit maturation stage. As compared with full irrigation, the average Δ_L at different growth stages and Δ_F at fruit maturation stage were decreased by 1.23% and 2.67% for different water deficit levels, respectively. Δ_L and Δ_F among different water deficit treatments had significant difference at the same growth stage (P < 0.05). Under different water deficit conditions, significant relationships between the Δ_L and WUE_i (photosynthesis rate/transpiration rate, P_n/T_r), WUE_n (photosynthesis rate/stomatal conductance of CO_2, P_n/g_s), WUE_y (yield/crop water consumption, Y/ET_c) and yield, or between the Δ_F and WUE_y and yield were found, respectively. There were significantly negative correlations of Δ_L with WUE_i, WUE_n, WUE_y and yield (P < 0.01) at the fruit maturation stage, or Δ_L with WUE_i and WUE_n (P < 0.01) over whole growth stage, respectively. Δ_F was negatively correlated with WUE_y, WUE_n and yield (P < 0.05), but positively correlated with ET_c (P < 0.01) over the whole growth stage. Thus Δ_L or Δ_F can compare WUE_n and WUE_y, so the stable carbon isotope discrimination method can be applied to evaluate the water use efficiency of pear-jujube tree under the regulated deficit irrigation.     

Expression of miRNA-22 in ovarian cancer and effect of miRNA-22 over-expression on SKOV-3 ovarian cancer cell proliferation,migration and invasion

AIM: To examine the expression of miRNA-22 in the ovarian tissues and the effect of miRNA-22 over-expression on the proliferation, migration and invasion in SKOV-3 cells. METHODS: The expression levels of miRNA-22 in different ovarian tissues and SKOV-3 cells were determined by qPCR. miRNA-22 was over-expressed by transfection of miRNA-22 mimic. The cell viability was examined by CCK-8 assay. The cell migration was measured by wound healing test. The cell invasion was analyzed by Transwell assay. The protein expression levels of VEGF and P53 were determined by Western blot. RESULTS: Compared with the normal ovarian tissue, the expression level of miRNA-22 was remarkably decreased in the ovarian tumor tissues. After transfection with miRNA-22 mimic, the expression level of miRNA-22 in the SKOV-3 cells was significantly increased, while the cell viability, migration and invasion were obviously decreased. Moreover, the protein expression of VEGF and P53 was dramatically inhibited after over-expression of miRNA-22. CONCLUSION: The decreased miRNA-22 expression may be correlated with the development of ovarian can-cer. Over-expression of miRNA-22 decreases the cell viability, migration and invasion by reducing the protein expression of VEGF and P53.     

以嗜酸乳杆菌发酵虾头虾壳回收蛋白质和甲壳素的研究

利用嗜酸乳杆菌发酵虾头、虾壳,结合虾头、虾壳中内源蛋白酶的自溶作用,回收蛋白质和甲壳素。研究了影响发酵的因素并优化了发酵条件,分析了用稀盐酸脱除粗甲壳素(即发酵残渣)中的矿物质,制备食用级甲壳素的条件。研究结果表明,添加15%葡萄糖,固液比1∶2.5,接种量15%,初始pH值6.5,于40℃发酵48 h,得到的虾头、虾壳发酵液pH值低至3.79,蛋白质水解度达到21.9%;蛋白质回收率达到95.2%,发酵残渣(粗甲壳素)中仅残留2.71%矿物质,达到工业级甲壳素的纯度要求;以稀盐酸浸泡处理粗甲壳素,当浸泡温度为50℃,浸泡时间为1.5 h,粗甲壳素脱矿物质的效果最好,甲壳素中仅残留0.2%矿物质,达到食用级甲壳素的纯度要求。     

硝酸还原酶的研究——Ⅴ.棉花硝酸还原酶活力与硝态氮含量的关系

棉花中的酚类物质对硝酸还原酶(NR)活力的体外测定有强烈抑制作用。用含半胱氨酸(5mM)和乙二胺四乙酸二钠(5mM)的磷酸缓冲液提取测不出酶活。如在磷酸缓冲液中加入酚类物质的抑制剂—聚乙烯吡咯烷酮(PVP),酶活力则显著增高。运用此法,本文在盆栽的情况下研究了棉花 NR 活力和硝态氮含量的关系。结果表明在不同棉花品种中、不     

关于黑龙江省森林食品资源开发利用的思考

通过对黑龙江省森林食品资源及产业现状的优势分析,提出了森林食品产业发展的措施及建议,以期为促进森林食品资源的合理开发利用,调整森林食品产业结构,增加林区经济收入提供参考。     

中国主要纸产品国际竞争力综合评价

文中在分析中国主要纸产品出口贸易现状的基础上,选取国际市场占有率、贸易竞争力指数和显示性比较优势指数测算中国主要纸产品2010-2019年的国际竞争力指数,并采用基于主成分分析的定权方法对纸产品国际市场竞争力进行综合评价。结果表明:主要纸产品国际竞争力较强的芬兰、瑞典和德国位居综合评价前3位,中国主要纸产品综合竞争力经过近10年发展已处于中等偏上水平,仍有改进和提升空间。基于上述研究成果,提出推进人工林速生材高效利用、促进科技创新能力提升和探索"出口转内销"等政策建议。     

不同制粒温度对植酸酶活性的影响及畜禽饲养对比试验研究

试验将含有制粒专用植酸酶制剂的日粮样本在4种温度条件下进行制粒．制粒后的饲料应用植酸酶国标法进行酶活测定,结果表明：制粒专用植酸酶在调制温度70℃左右、蒸汽压力不高的情况下酶活基本未丧失,性能比较理想：当制粒温度达到80℃以上时,酶活明显降低,但总体保持在500U／kg以上．制粒用植酸酶仍具有较好的耐热性能。经过动物饲养试验表明．肉鸡和育肥猪饲养对照组和处理组之间的平均日增重和料肉比差异都不显著（p〉0．01）,这说明在添加制粒专用植酸酶后．对磷酸氢钙的量进行调整并不会影响肉鸡和育肥猪的生长性能．且同样能达到普通植酸酶在粉状饲料中的应用效果。     

鸽载脂蛋白B基因多态性与生长性状相关性分析

利用PCR-SSCP技术和DNA测序方法检测两群体鸽apoB基因编码区序列的单核苷酸多态性,最小二乘分析表明,A57G突变位点导致的3种基因型对石岐肉鸽屠体率及灰色肉鸽屠体重、腹脂率、肝脏重有显著影响(P<0.05);对灰色肉鸽活重、腹脂重、肝脏率及石岐肉鸽活重和全净膛率有一定影响(P<0.2).多重比较显示,石岐肉鸽群体中,BB基因型个体的屠体率高于从基因型个体.灰色肉鸽群体中,BB基因型个体的屠体重、肝脏重、腹脂率均为最大.