Light and electron microscopic studies of calf kidneys after exposure to subtoxic lead levels]

Eight calves were fed from the fourth to the twentieth week of age a milk powder diet containing 40 mg lead acetate per kg dry substance. This is twice the lead concentration legally permitted. In average, each animal has daily taken up 0.834 mg lead per kg body weight. Eight calves were used as controls. The animals gained the usual fattening weights and did not show clinical symptoms. Beginning with week 14, increased levels of blood urea were encountered in the animals which received lead in their diet. Morphologically, severe renal lesions were found in these calves. The kidneys were increased in weight, pale and of firm consistency. Histologically, fibrosis and periglomerular interstitial non-purulent nephritis were found. Light- and electronmicroscopically, numerous intranuclear inclusion-bodies typical for lead poisoning were demonstrated in epithelial cells of proximal tubules. The results show, that daily uptake of less than 1 mg lead per kg body weight must be considered as a toxic dose for calves, and not 5-7 mg as stated in literature.     

湖南省茶树黑刺粉虱寄生蜂调查

在湖南调查茶园黑刺粉虱的寄生蜂,共获5种。其中,长角广腹细蜂Amitus longicornis(?),粉虱长棒蚜小蜂东方型Eretmoeerus serius var orientalis Silvestri,粉虱蚜小蜂TrichaPorus formosus(Gah.)及黄盾粉虱蚜小蜂Encarsia sP.系中国新记录种。另一种为长腹扑虱蚜小蜂Prospaltella ishii Silvestri,系湖南新记录种。本文分别记述了它们的形态、寄主、分布及习性观察结果。     

遗传背景对水稻无融合生殖材料C1001双胚苗频率影响的研究

同核异质雄性不育材料与无融合生殖材料Ｃ１００１Ｂ杂交和回交表明，Ｃ１００１Ｂ的双胚苗，是受核基因控制的性状，双胚苗频率的表达，具明显的胞质效应和胞核效应，在胞质效应中，不育胞质与可质胞质的差异尤其显著，不育胞质对双胚苗的表现有明显的抑制作用，不同世代间胞质效应和胞核效应的表现也不一致。此外，环境条件对Ｃ１００１的双胚苗表现有一定的影响。     

Enzootic abortion in a goat herd, caused by mixed infection with Coxiella burnetii and Chlamydia psittaci. Case report]

We report on an outbreak of enzootic abortion in a herd of goats with high losses caused by mixed infection with Coxiella burnetii and Chlamydia psittaci. Both agents could be detected by microscopic investigation of smears from afterbirths by Giménez staining and by a capture enzyme-linked immunofluorescence assay (Capture ELIFA). Additionally, Coxiella burnetii was isolated from the same tissues in BGM cell cultures. Serological investigations with CF, indirect ELIFA, and IF tests revealed high antibody titers against both agents in most animals. Coxiella antigen was still detected 91 days later in milk samples of two infected goats by Capture ELIFA.     

猪肉毒梭菌毒素中毒的诊断报告

１９９９年７月１５日，遵化市某养猪场的６０ｋｇ左右的育肥猪，因饲喂霉变的小干鱼，引起猪只急性发病和死亡。发病猪只７５头，死亡２１头，治愈５４头，治愈率７２％，现将情况报告如下。１ 临床症状 病猪呆立，食欲废绝，精神沉郁，鼻端、眼结膜发绀，口角流涎并有泡沫，呼吸困难，腹壁松驰，有的腹泻，粪便呈黄绿色或灰绿色水样物，站立不稳或颤抖，尾下垂，不走动，叫声嘶哑，反应迟钝。后肢多呈瘫痪状，卧地不起，最后瞳孔散大，窒息死亡。病猪大多体温正常，个别达４０～４１℃。病程一般在１～３ｄ，个别猪发病到死亡仅 ５～６ ｈ…     

法氏囊活性肽对鸡体增重及饲料转化率的影响

把不同剂量的法氏囊活性肽 (BS)冻干粉与传染性法氏囊病 (IBD)活苗混合后 ,对 2 1日龄SPF鸡滴鼻点眼 ,同时另选一批同样生长状态的鸡 ,在颈部皮下接种IBD细胞毒油苗 ,同时肌肉注射上述不同剂量法氏囊活性肽 ,观察法氏囊活性肽对鸡生长性能的影响。结果发现 :免疫后7d ,活苗 0 2mLBS组增长最快 ,比免疫对照组多增 82 5 % ,并且饲料报酬高。但从整个试验期看 ,0 4mLBS组增重效果较好 ,比免疫对照组多增 1 9 8% ,并且有较高的饲料转化率。而肌肉注射BS组中 ,高剂量 (0 8mL)BS组的增重效果一直很好 ,比对照组总增重平均多增 7 2 3 % ,并有较高的饲料报酬。这说明滴鼻点眼或是肌肉注射 ,BS都有促生长的作用     

中国的枸橼

枸橼在我国有11个类型(变种、品种或杂种),根据它们的地理分布和植物学性状,认为枸橼的原发中心在云南的西南部和西部;野生的沧源香橼是枸橼的原始类型,由其演化为小香橼、小果香橼和栽培的大香橼等;佛手是由枸橙演化而来。     

油气管道风险管理技术现状及对策

介绍了国内外油气管道风险管理技术的研究现状、应用成果及最新的发展趋势,对比分析了国内外油气管道风险管理技术的差距,强调了油气管道风险管理的重要性,指出了油气管道风险技术的发展方向,为油气管道安全运行提供了技术支持.     

呼和浩特市南郊大气污染源调查的分析

呼和浩特市南郊大气污染源调查的分析杨秋林，郭玲，杨性恺，席延钰（内蒙古农牧学院基础课部，呼和浩特市，０１００１８）摘要调查了呼市南郊大气污染源的现状，分析了主要污染源和污染物，提出了对呼市南郊大气污染的防治对策和建议。关键词大气，大气污染，污染源，污...     

Differential effects of heparin on NO and tumor necrosis factor-alpha production in bovine blood mononuclear cells stimulated with Salmonella typhimurium lipopolisaccharide.

We investigated the influence of heparin, one of the extracellular matrix (ECM) components, on nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) production by bovine peripheral blood mononuclear cells (PBMC) and monocytes left to adhere for 2 (freshly adherent monocytes) and 48 h (resting monocytes), activated with Salmonella typhimurium lipopolysaccharide (LPS). After 24-h stimulation with LPS, heparin (100 microg/ml) increased (by about 40%) NO production by peripheral blood mononuclear cells and by freshly adherent monocytes. However, it did not change NO synthesis by the resting monocytes. Unlike its influence on NO level, heparin diminished TNF-alpha production by PBMC and monocytes stimulated with LPS. Microscopical examination of PBMC stained with biotin-labeled heparin, showed that both lymphocytes and monocytes were able to bind this glycosaminoglycan. We suggest that heparin, as a component of ECM, modulates the early response of monocytes to exogenous stimuli.