Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province

In order to study and analyze L1 gene of bovine papillomavirus（BPV）in Guizhou province,the L1 gene of BPV-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,and the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis, transmembrane domain and signal peptide of L1 gene were predicted.The results showed that the length of L1 gene was 1 494 bp,encoding 497 amino acids.The L1 gene of BPV-GZ01 strain shared an amino acid identities of 98.6%,99.4%,98.4%,94.4% and 91.3%,and a nucleotide identities of 99.1%,99.8%,99.4%,87.6% and 82.8% with those of BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1 strains,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between BPV-GZ01 and BPV2-SW01 strains.The prediction of secondary structure of L1 protein indicated that the random coil,extended strand and alphahelix took a higher percentage.The L1 protein was supposed contain 6 potential antigen epitopes.And no transmembrane domains and no signal peptide were found.The tertiary structure of L1 protein was curved spiral structure.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of BPV.     

Isolation and Identification of Pathogenic Bacteria Causing Dairy Cow Mastitis in Liaoning and Drug Sensibility Test of Pathogenic E.coli

In order to understand the main bacterial pathogen species causing dairy cow mastitis in Liaoning, as well as the characteristics of drug sensitivity of the pathogenic E.coli,the milk samples from 75 dairy cows with clinical manifestations for mastitis in certain large-scale dairy farm in Liaoning were collected.The bacteria in milk were cultured and isolated with biochemical methods and in vitro drug sensitivity tests were processed with the isolated E.coli strains.The results showed that the main bacterial pathogen for dairy cow mastitis were E.coli（separation rate 58.7%),S.aureus（64.0%）and S.agalactiae（54.7%),and multiple infection including double and triple infection were identified.The drug sensitivity tests on the isolated E.coli indicated that the E.coli isolates were highly resistant to sulfonamides（resistance rate>85%）and chloramphenicol（resistance rate>30%),and they were relatively low resistant to ampicillin（9.5%),ciprofloxacin（9.5%),ceftiofur（7.1%）and ofloxacin（4.8%).The results was able to provide reliable theoretical basis for prevention and control of dairy cow mastitis in Liaoning area.     

“赤霞珠”葡萄组培快繁体系研究

以"赤霞珠"葡萄为试材,对组培快繁中的取材部位、消毒方法、不同激素用量、是否带叶继代培养等关键技术措施进行了研究探讨。结果表明:以半木质化,0.1%升汞溶液消毒8min的茎段接种,成活率高,污染率低;并且继代培养和生根培养最好的培养基为1/2MS+IBA0.2mg·L~(-1)+蔗糖20g·L~(-1)+琼脂7g·L~(-1)。     

避雨栽培对“户太八号”葡萄生长及果实发育的影响

以"户太八号"葡萄为试材,比较了露地栽培与避雨栽培2种模式下葡萄的生长发育状况、外观品质及理化性质。结果表明:避雨栽培极显著削弱了叶幕层光照强度,为常规栽培的68.71%;避雨栽培下葡萄日平均净光合速率降低12.10%,叶绿素含量平均降低5.57%;在叶幕微气候方面,避雨栽培下叶幕层日平均温度提高了1.68℃,日平均湿度降低了10.98%。与常规栽培比较,避雨栽培平均果粒质量降低了1.52g,平均果穗质量降低了92g;避雨栽培下葡萄的可溶性固形物高于常规栽培,差异不显著;含酸量在果实发育时期极显著低于常规栽培下的葡萄,且差值较大。避雨栽培可显著提高果实固酸比,提高果实品质。     

水分胁迫对幼龄仁用杏叶片光合特性的影响

以2年生仁用杏为试材,采用盆栽方法,以正常供水(土壤含水量为田间最大持水量的75%)为对照,设置了轻度水分胁迫(55%)、中度水分胁迫(45%)和重度水分胁迫(30%)3个处理,开展了水分胁迫条件下仁用杏叶片光合色素、光合指标及叶绿素荧光参数等光合特性研究。结果表明:水分胁迫不同程度降低了类胡萝卜素含量、叶绿素a、b含量、净光合速率(Pn)、蒸腾速率(Tr)、水分利用效率(WUE)、最大荧光产量(Fm)、最大光化学效率(Fv/Fm)和PSⅡ潜在活性,且下降幅度随着胁迫时间延长而增大;轻度水分胁迫下降幅度很小,中度和重度水分胁迫下降幅度较大。     

白兰修剪截口愈合生长研究

以白兰(Michelia alba)为试材,于2013年12月对其10~20、20~30、30~40mm这3个直径的枝条进行疏枝试验,并于2014年3、6、9、12月跟踪测量截口的愈合程度,研究了截口直径、季度变化对其愈合的影响,以期为园林树木修剪工作提供理论依据。结果表明:截口愈合速度与园林树木年生长物候相一致;20~30mm直径的截口愈合效果明显优于另2个直径的截口,是较理想的疏枝直径。     

珍稀濒危植物膝柄木种子催芽研究

以采自越南广宁省海河县的膝柄木种子为试材,研究了不同药剂和浓度对种子发芽率和芽苗主根生长的影响。结果表明:与水浸相比,艾比蒂生根剂1号和双吉尔氨基酸肥料6号0.5~15.0mg/L浸种1h,对膝柄木种子发芽均没有促进作用,可见水浸即可有效促进膝柄木种子发芽;艾比蒂生根剂1号0.5mg/L和双吉尔氨基酸肥料6号0.5~10.0mg/L对膝柄木芽苗主根生长有很好的促进作用。     

我国果树玻璃化试管苗特征、成因、机理及控制研究进展

该研究统计了我国已报道的出现玻璃化试管苗的果树种类多达50种,并基于近年果树工作者的相关研究,总结并系统论述了玻璃化试管苗的生理生化特性、形态解剖学特征、DNA的MSAP分析、成因、发生机理及控制措施,对系统研究果树试管苗玻璃化现象、增加转基因植株的遗传稳定性、保存和恢复珍贵材料无性系、提高次生代谢物产量和消除毒性及工厂化育苗都具有十分重要的意义。     

赤霉素和磷肥配合施用对胡麻光合酶活性及其产量形成的影响

为探究赤霉素和磷肥配合施用对胡麻叶片光合酶活性以及籽粒产量的影响,以‘轮选2号’为材料,采用田间二因素裂区试验设计,研究了不同磷肥用量水平(P₀:0 kg·hm^-2 P₂O₅,P₁:67.5 kg·hm^-2 P₂O₅,P₂:135 kg·hm^-2 P₂O₅)和赤霉素(Gibberellin acid, GA₃)喷施浓度(G₀:0 mg L^-1,G₁:15 mg·L^-1,G₂:30 mg·L^-1)对胡麻光合同化酶活性、干物质积累量及籽粒产量的影响。结果表明：施磷和喷施赤霉素均显著提高了胡麻叶片RuBP(Ribulose-1,5-bisphosphate)、PEP(Phospho...     

我国小麦茎基腐病菌致病力比较分析

为明确我国不同种、地理来源和毒素化学型小麦茎基腐病菌的致病力分化情况,采用纸塔法对来自全国9个省市80个采样点分离的224株小麦茎基腐病菌进行致病力分析。结果表明,不同种镰刀菌的致病力不同,黄色镰刀菌Fusarium culmorum,禾谷镰刀菌F.graminearum,假禾谷镰刀菌F.pseudograminearum及亚洲镰刀菌F.asiaticum致病力强于其他种。F.culmorum致病力显著高于F.pseudograminearum和F.asiaticum,而F.pseudograminearum,F.graminearum及F.asiaticum三者之间无显著性差异。中华镰刀菌F.sinensis,木贼镰刀菌F.equiseti,锐顶镰刀菌F.acuminatum致病力较弱,三者间苗期致病力无显著性差异;多数省份F.pseudograminearum群体间致病力无显著差异,仅山东F.pseudograminearum群体的致病性显著低于河南群体;此外,产毒类型为3ADON的F.pseudograminearum群体致病力显著高于15ADON群体。