全文获取类型
收费全文 | 50972篇 |
免费 | 2737篇 |
国内免费 | 33篇 |
专业分类
林业 | 2622篇 |
农学 | 1544篇 |
基础科学 | 217篇 |
5748篇 | |
综合类 | 10151篇 |
农作物 | 1949篇 |
水产渔业 | 2346篇 |
畜牧兽医 | 25468篇 |
园艺 | 559篇 |
植物保护 | 3138篇 |
出版年
2019年 | 451篇 |
2018年 | 735篇 |
2017年 | 789篇 |
2016年 | 687篇 |
2015年 | 636篇 |
2014年 | 767篇 |
2013年 | 1759篇 |
2012年 | 1486篇 |
2011年 | 1666篇 |
2010年 | 1074篇 |
2009年 | 1163篇 |
2008年 | 1633篇 |
2007年 | 1647篇 |
2006年 | 1539篇 |
2005年 | 1466篇 |
2004年 | 1379篇 |
2003年 | 1349篇 |
2002年 | 1283篇 |
2001年 | 1530篇 |
2000年 | 1485篇 |
1999年 | 1215篇 |
1998年 | 456篇 |
1997年 | 476篇 |
1996年 | 473篇 |
1995年 | 524篇 |
1994年 | 472篇 |
1993年 | 482篇 |
1992年 | 925篇 |
1991年 | 919篇 |
1990年 | 914篇 |
1989年 | 920篇 |
1988年 | 868篇 |
1987年 | 899篇 |
1986年 | 890篇 |
1985年 | 894篇 |
1984年 | 724篇 |
1983年 | 653篇 |
1979年 | 647篇 |
1978年 | 540篇 |
1977年 | 459篇 |
1976年 | 462篇 |
1975年 | 503篇 |
1974年 | 692篇 |
1973年 | 605篇 |
1972年 | 675篇 |
1971年 | 671篇 |
1970年 | 598篇 |
1969年 | 590篇 |
1968年 | 450篇 |
1967年 | 511篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
421.
422.
423.
424.
Human–Dromedary Camel Interactions and the Risk of Acquiring Zoonotic Middle East Respiratory Syndrome Coronavirus Infection 下载免费PDF全文
C. Gossner N. Danielson A. Gervelmeyer F. Berthe B. Faye K. Kaasik Aaslav C. Adlhoch H. Zeller P. Penttinen D. Coulombier 《Zoonoses and public health》2016,63(1):1-9
Middle East respiratory syndrome coronavirus (MERS‐CoV) cases without documented contact with another human MERS‐CoV case make up 61% (517/853) of all reported cases. These primary cases are of particular interest for understanding the source(s) and route(s) of transmission and for designing long‐term disease control measures. Dromedary camels are the only animal species for which there is convincing evidence that it is a host species for MERS‐CoV and hence a potential source of human infections. However, only a small proportion of the primary cases have reported contact with camels. Other possible sources and vehicles of infection include food‐borne transmission through consumption of unpasteurized camel milk and raw meat, medicinal use of camel urine and zoonotic transmission from other species. There are critical knowledge gaps around this new disease which can only be closed through traditional field epidemiological investigations and studies designed to test hypothesis regarding sources of infection and risk factors for disease. Since the 1960s, there has been a radical change in dromedary camel farming practices in the Arabian Peninsula with an intensification of the production and a concentration of the production around cities. It is possible that the recent intensification of camel herding in the Arabian Peninsula has increased the virus' reproductive number and attack rate in camel herds while the ‘urbanization’ of camel herding increased the frequency of zoonotic ‘spillover’ infections from camels to humans. It is reasonable to assume, although difficult to measure, that the sensitivity of public health surveillance to detect previously unknown diseases is lower in East Africa than in Saudi Arabia and that sporadic human cases may have gone undetected there. 相似文献
425.
The Epidemiology and Characteristics of Q fever and Co‐infections with Scrub Typhus,Murine Typhus or Leptospirosis in Taiwan: A Nationwide Database Study 下载免费PDF全文
C.‐H. Lai W. Sun C.‐H. Lee J.‐N. Lin M.‐H. Liao S.‐S. Liu T.‐Y. Chang K.‐F. Tsai Y.‐C. Chang H.‐H. Lin Y.‐H. Chen 《Zoonoses and public health》2017,64(7):517-526
Q fever (QF) is a worldwide zoonosis associated with outbreaks. Only a few nationwide studies regarding the surveillance and epidemiology of human QF have been reported. Although QF is endemic in Taiwan, a nationwide database investigation of the epidemiology and characteristics of QF and its associations with scrub typhus (ST), murine typhus (MT) and leptospirosis (LS) has never been reported. We analysed nationwide databases of suspected QF, ST, MT and LS cases from October 2007 to December 2014 obtained from the Centers for Disease Control, Taiwan. A total of 468 (4.2%) QF cases were identified among 11 109 suspected QF cases. QF cases were mainly distributed in the southern and Kaohsiung–Pingtung regions but rarely in the eastern region. Compared to non‐QF cases, QF cases had significantly higher percentages of males (88.7 versus 66.2%) and high‐risk occupations (farming, animal husbandry or veterinary medicine) (16.2 versus 10.5%). But the percentages of specific animal contact, including cattle (0.6 versus 0.8%) and goats (0.9 versus 1.0%), were low in both. The majority of suspected QF cases (89.4%) were simultaneously suspected with ST, MT or LS, and the combinations of suspected diseases differed between regions. The number of suspected QF cases from the eastern region decreased since 2009, which was not observed in other regions. A total of 1420 (12.8%) cases had confirmed diseases, including QF (453, 4.1%), QF+ST (7, 0.06%), QF+MT (4, 0.04%), QF+LS (4, 0.04%), MT (186, 1.7%), ST (545, 4.9%), ST+LS (11, 0.1%) and LS (210, 1.9%). Compared to cases of unknown disease, QF cases had larger percentages of high‐risk occupations (16.2 versus 9.6%) but similar histories of animal contact (29.8 versus 25.1%). QF is an endemic disease in southern Taiwan. It is difficult to differentiate QF from ST, MT or LS only by high‐risk occupations and history of animal contact, and co‐infection of QF with these diseases should be considered. 相似文献
426.
427.
Detection of challenge virus in fetal tissues by nested PCR as a test of the potency of a porcine parvovirus vaccine 总被引:1,自引:0,他引:1
Belák S. Rivera E. Ballagi-Pordány A. Hanzhong W. Widén F. Soós T. 《Veterinary research communications》1998,22(2):139-146
To estimate the potency of a porcine parvovirus (PPV) vaccine, three vaccinated and three non-vaccinated pregnant gilts were infected with PPV and the distribution of the virus was studied in the tissues of their 51 fetuses. Virus detection was attempted using haemagglutination (HA) and immunofluorescence (IF) assays, as well as by standard (single) and nested polymerase chain reactions (PCR). None of the detection methods yielded positive results when used to test for the presence of virus in suspensions of organs from the fetuses from the vaccinated gilts. However, the virus was detected in the fetuses from non-vaccinated gilts as follows: HA was positive in 14 cases out of 23 (60.8%), IF in 16/23 (69.5%), standard PCR in 12/20 (60%), and the nested PCR in 19/23 (82.6%). Although the correlation among the results of various methods of virus detection was rather close (r<0.83), the sensitivity of the nested PCR was the highest, both when testing dilutions of PPV and when analysing the fetal organs. The nested PCR therefore provides a reliable approach for studies of virus distribution in fetal organs, with special reference to potency tests on vaccines. 相似文献
428.
Daniel D. Gallaher Kimberly J. Wood Cynthia M. Gallaher Leonard F. Marquart Alta M. Engstrom 《Cereal Chemistry》1999,76(1):21-24
The intestinal contents viscosities of oat-based breakfast cereals and muffins were examined. Male Sprague-Dawley rats were adapted for four days to a semipurified diet (AIN-76A). Following an overnight fast, the animals were meal-fed 5 g of either the AIN-76A diet (containing 5% cellulose), the AIN-76A diet containing 2% guar gum, whole-grain oat flour, one of five cereals (corn flakes, cooked oatmeal, uncooked oatmeal, cooked oat bran, or Cheerios), or one of two types of muffins (containing whole-grain oat flour or oatmeal). Two hours after presentation of the meal, the animals were killed, the small intestines removed, and the contents collected. The contents were centrifuged, and the viscosity values of the undiluted supernatants were determined. The supernatant viscosity from rats fed the AIN-76A diet was negligible (<5 mPa·sec), whereas that from rats fed guar gum was high (396 ± 117 mPa·sec). Of the cereals fed, corn flakes resulted in the lowest viscosity (<5 mPa·sec). However, all oat-based cereals resulted in high intestinal contents supernatant viscosity levels (cooked oatmeal 368 ± 128, uncooked oatmeal 307 ± 107, cooked oat bran 301 ± 85, Cheerios 199 ± 58, mPa·sec) with no statistically significant differences. The intestinal contents viscosity values for the whole-grain oat flour muffin and oatmeal muffin were 233 ± 52 and 111 ± 26 mPa·sec, respectively, a statistically significant difference (P < 0.05). This suggests that the form of the oat within a food may influence the degree of viscosity produced within the small intestine after that food is consumed. 相似文献
429.