浅谈奶牛子宫内膜炎

随着人们生活水平的不断提高。我国乳业在迅速的发展,奶牛养殖业也在大规模地扩增。奶牛的不孕症是奶牛四大类疾病之一,而子宫内膜炎是奶牛不孕症的主要原因之一。子宫内膜炎是奶牛生产中的常见病,一旦发生,奶牛配种困难或长期不孕,导致养殖效率低,甚至提前淘汰。抓好子宫内膜炎的预防和治疗,对促进奶业增效、奶农增收意义重大。据报道,我国奶牛子宫内膜炎型不孕症占成年母牛不孕症的17％以上。每年美国有12．19％的奶牛患有繁殖疾病。经济损失近2．5亿美元;英国报道的不孕牛中约95％是由于子宫内膜炎引起的。在国内,1984～1988年,中国农业科学院中兽医研究所对10个城市30个奶牛场的10495头适龄母牛的调查表明,产后患子宫内膜炎的有2107头．发病率为20．08％。瞿自明等1983～1985年对北京、上海、南宁、兰州等16个城市41个奶牛场调查了9754头适龄奶牛,有1684头发生子宫内膜炎,约占17．26％,占不孕牛的68．34％。由此可见,奶牛子宫内膜炎对畜牧业所造成的经济损失是相当严重的。     

蜡样芽孢杆菌及其生物被膜对有机酸及乙醇的抗性比较

为比较蜡样芽孢杆菌及其生物被膜对环境压力的抗性,该文主要研究了4种有机酸（乙酸、柠檬酸、乳酸和苹果酸）和乙醇对蜡样芽孢杆菌及其生物被膜存活率的影响。结果表明：生物被膜态蜡样芽孢杆菌对乙酸的抗性高于浮游态菌。扫描电镜结果显示,经乙酸处理后,浮游态蜡样芽孢杆菌的细胞表面严重受损,而生物被膜态菌的表面形态未发生明显变化。在柠檬酸、乳酸、苹果酸和乙醇存在的条件下,生物被膜态蜡样芽孢杆菌比浮游态菌表现出更强的压力抗性,特别是在高浓度（有机酸16%～20%,乙醇50%～60%）的情况下,该现象尤为显著。因此,在食品工业中控制被膜态蜡样芽孢杆菌对预防和阻止食品腐败非常重要。该研究结果为实际生产中有效控制蜡样芽孢杆菌及其生物被膜的形成提供参考。     

Soil Redistribution investigations by combined use of soil 137Cs and selected chemical properties

Abstract

To improve the methods of application of phosphorus or supply of soil P to Azolla (A. microphylld), basal application, split application, inoculation of P-enriched Azolla, and soil disturbance were compared. Soil disturbance did not increase the floodwater P content. Phosphorus was applied to inoculum production plots to enrich Azolla with P. Thus, phosphorus-enriched Azolla could multiply 5–7 times after inoculation until it became P deficient. Trials on the methods of enrichment of Azolla with P showed that the best method was to broadcast twice 4.33 kg P (10 kg P₂O₅/ha) at 2-day intervals and to harvest Azolla 3 days after P application. Addition of P once or twice 2 weeks after the inoculation of P-enriched Azolla further increased the biomass production. Efficiency of P application was analyzed in terms of N gain in relation to the amount of P applied. This ratio in the P-enriched Azolla treatment was higher than the economically sound ratio -5-, and higher than or equal to that in the standard split application.     

Assessment of intake inadequacy and food sources of zinc of people in China

OBJECTIVES: To assess the intake inadequacy and food sources of zinc of people in China. DESIGN AND SUBJECTS: Diets of 68 962 subjects aged 2-101 years (urban 21 103, rural 47,859) in the 2002 China National Nutrition and Health Survey were analysed. Dietary intake was assessed using 24-hour recall for three consecutive days. Zinc intake inadequacy was calculated based on values suggested by the World Health Organization. RESULTS: The median zinc intake ranged from 4.9 mg day- 1 (urban girls, 2-3 years) to 11.9 mg day-1 (rural males, 19+ years). The zinc density of urban residents (2-3 to 19+ years) was 5.0-5.3 mg day-1 (1000 kcal)-1, significantly higher than that of their rural counterparts (4.7-4.8 mg day-1 (1000 kcal)-1). Differences in food sources of zinc from cereal grains (27.4-45.1 vs. 51.6-63.2%) and animal foods (28.4-54.8 vs. 16.8-30.6%) were found between urban and rural residents. Zinc from vegetables and fruits (8.2-13.8 vs. 9.7-12.4%) and legumes (1.3-3.3 vs. 2.5-3.4%) was comparable between urban and rural residents. The proportion of zinc intake inadequacy ranged between 2.8% (urban females, 19+ years) and 29.4% (rural lactating women). Rural residents had higher proportions of zinc intake inadequacy than their urban counterparts. Significantly higher proportions of zinc inadequacy were found in the category of phytate/zinc molar ratio >15 for both rural and urban residents. CONCLUSIONS: About 20% of rural children are at risk of inadequate zinc intake, with phytate as a potential important inhibitor. Moreover, lactating women are also considered a vulnerable group.     

Levels of active oxygen species are controlled by ascorbic acid and anthocyanin in Arabidopsis

Stabilization of the levels of active oxygen species (AOS) is important to the survival of organisms. To clarify the system controlling levels of AOS in plants, this study used an electron spin resonance (ESR) method to directly measure superoxide radical (O(2)(.-)) scavenging activities in the wild-type Arabidopsis thaliana (Col and Ler ecotypes), two anthocyanin mutants (tt3 and ttg1), and an ascorbic acid mutant (vtc1). Under ordinary growth conditions, Arabidopsis contained superoxide-scavenging activity (SOSA) of approximately 300-500 SOD units/g of fresh weight. The ESR pattern indicated that most (40-50%) of this activity was due to ascorbic acid. For the analysis of SOSA under conditions of oxidative stress, synthesis of AOS was induced by gamma-irradiation. The radical scavenging activity in irradiated plants increased approximately 10-fold following an associated increase in the accumulation of ascorbic acid and anthocyanin. The accumulation of ascorbic acid and anthocyanin was suppressed by treatment with an antioxidant before irradiation and was induced by treatment with a radical-generating reagent. The contributions of ascorbic acid and anthocyanin to the total superoxide radical scavenging activity differed among ecotypes. In the Ler ecotype, ascorbic acid accumulated at twice the level of that in the Col ecotype, and induction of anthocyanin was half that in Col. To confirm the activity of ascorbic acid and anthocyanin against AOS stress, the viability of the wild type and mutants (tt2, tt3,tt5, ttg1, and vtc1) was examined after gamma-irradiation. Only the plants in which ascorbic acid and anthocyanin were induced had the ability to grow and flower.     

发生分类淋溶土与系统分类参比特征研究

利用最新建立的中国 1∶10 0万土壤数据库 ,研究了我国发生分类淋溶土与中国土壤系统分类的参比及其在中国土壤系统分类下的空间分布和数量特征。结果表明 ,我国发生分类淋溶土总面积为10 5 975 7.8km2 ,分布规律明显 ;与系统分类参比 ,发生分类淋溶土分属于中国土壤系统分类 4个土纲 ,即淋溶土、雏形土、新成土和灰土 ,它们分别占发生分类淋溶土总面积的 72 .8%、2 6 .1%、1.0 %、0 .1% ,其中包含系统分类的 2 5个土类和 4 7个亚类 ,参比关系复杂 ,不是简单的一对一的关系。分析发生分类某一类型土壤分属于系统分类不同类型的面积比例及其标准偏差 ,结果表明土壤参比的单元级别越低 ,越易于参比和把握。为了使中国土壤系统分类更实用、更易于普及 ,深入开展土壤基层分类研究 ,进一步发展和完善中国土壤系统分类是必要的 ,也是十分迫切的。研究结果对于土壤类型的正确参比、中国土壤系统分类的应用与发展 ,具有很好的参考应用价值     

作物根系分区交替灌溉田间实施技术探索

作物根系分区交替灌溉方法的提出开辟了节水灌溉的新思路,该方法具有明显的节水、增产、提高水分利用效率的优点.目前对它的田间实施技术研究还不全面、不系统.本文针对其特点,探索分析能够实现根系分区交替灌溉目的的灌水技术种类、技术要点及适用条件,提出适合目前我国农村经济发展现状的根系分区交替灌溉田间实施技术,并对未来的技术发展提出了研究思路.     

稻瘟病拮抗菌NK413的鉴定及抑菌效果

菌株NK413是从黑龙江省农田土壤中分离得到的一株放线菌,为明确其分类地位及在植物病害生物防治中的应用潜力,采用多相分类法对其进行鉴定,并采用孢子萌发抑制法、菌丝生长速率法、杯碟法考察了NK413菌株及其代谢产物对稻瘟病菌的抑菌效果及作用方式。结果表明:菌株NK413的形态特征、生理生化特征与小白链霉菌(Streptomyces albulus)接近,细胞壁类型为Ⅰ型、磷脂脂肪酸成分符合链霉菌属特征。16S rDNA全长为1 614 bp,与小白链霉菌亲缘关系最近,因此将NK413定名为小白链霉菌(S.albulusNK413)。NK413对稻瘟病菌孢子萌发和菌丝生长的抑制作用较强,原液对稻瘟病菌的抑菌圈直径达30 mm,无菌发酵滤液稀释1倍对孢子萌发的抑制率为100%,稀释5倍时对菌丝生长的抑制率为100%。抗菌谱广,具有较好的开发前景。     

杂交小麦品种绵杂麦168稀植高效栽培技术初探

为解决杂交小麦生产用种量大,种子生产成本高这一难题,加速杂交小麦新品种在生产上的推广应用,特就施氮量与密度对绵杂麦168产量的影响进行了研究。试验采用两因素裂区设计,施氮量为主区,密度为副区,施氮量与密度各5水平。结果表明,施氮量、密度以及施氮量与密度的互作都极显著地影响绵杂麦168的产量。产量随着施氮量的增加而呈增加趋势;产量随着密度的增加也呈增加趋势,但在密度极高的情况下,产量又有所下降。在基础地力中等水平的情况下,在低用种量（密度≤165×104基本苗／hm2）下,在施氮量为150～300kg／hm2情况下,种植绵杂麦168可获得高于6750kg/hm2的产量和高于8000元／hm2的经济效益。