全文获取类型
收费全文 | 328篇 |
免费 | 21篇 |
专业分类
林业 | 25篇 |
农学 | 23篇 |
基础科学 | 6篇 |
56篇 | |
综合类 | 47篇 |
农作物 | 16篇 |
水产渔业 | 15篇 |
畜牧兽医 | 120篇 |
园艺 | 11篇 |
植物保护 | 30篇 |
出版年
2023年 | 2篇 |
2021年 | 2篇 |
2020年 | 5篇 |
2019年 | 5篇 |
2018年 | 7篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 13篇 |
2014年 | 5篇 |
2013年 | 21篇 |
2012年 | 11篇 |
2011年 | 16篇 |
2010年 | 16篇 |
2009年 | 14篇 |
2008年 | 18篇 |
2007年 | 22篇 |
2006年 | 18篇 |
2005年 | 22篇 |
2004年 | 23篇 |
2003年 | 14篇 |
2002年 | 21篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 8篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1985年 | 2篇 |
1984年 | 5篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1960年 | 1篇 |
1955年 | 1篇 |
1953年 | 1篇 |
1945年 | 1篇 |
排序方式: 共有349条查询结果,搜索用时 15 毫秒
141.
Bacterial-derived lipopolysaccharides (LPS) can cause defective intestinal barrier function and play an important role in the development of inflammatory bowel disease. In this study, a nanocarrier based on chitosan and fucoidan was developed for oral delivery of berberine (Ber). A sulfonated fucoidan, fucoidan-taurine (FD-Tau) conjugate, was synthesized and characterized by Fourier transform infrared (FTIR) spectroscopy. The FD-Tau conjugate was self-assembled with berberine and chitosan (CS) to form Ber-loaded CS/FD-Tau complex nanoparticles with high drug loading efficiency. Berberine release from the nanoparticles had fast release in simulated intestinal fluid (SIF, pH 7.4), while the release was slow in simulated gastric fluid (SGF, pH 2.0). The effect of the berberine-loaded nanoparticles in protecting intestinal tight-junction barrier function against nitric oxide and inflammatory cytokines released from LPS-stimulated macrophage was evaluated by determining the transepithelial electrical resistance (TEER) and paracellular permeability of a model macromolecule fluorescein isothiocyanate-dextran (FITC-dextran) in a Caco-2 cells/RAW264.7 cells co-culture system. Inhibition of redistribution of tight junction ZO-1 protein by the nanoparticles was visualized using confocal laser scanning microscopy (CLSM). The results suggest that the nanoparticles may be useful for local delivery of berberine to ameliorate LPS-induced intestinal epithelia tight junction disruption, and that the released berberine can restore barrier function in inflammatory and injured intestinal epithelial. 相似文献
142.
Tammi S. Epp Paul McDonough Don E. Myers Danielle J. Carlin Brad J. Behnke Casey A. Kindig David C. Poole Howard H. Erickson 《Journal of Equine Veterinary Science》2009
Inflammatory airway disease has been linked to exercise-induced pulmonary hemorrhage (EIPH), and consequently, we hypothesized that immunomodulation via concentrated equine serum (CES) treatment would reduce EIPH as evidenced by red blood cell (RBC) concentrations in bronchoalveolar lavage fluid (BALF). Separate trials were conducted on Thoroughbred horses treated with either CES (n = 6) or placebo (0.9% saline; n = 4). All horses completed pre-treatment and post-treatment (2 and 4 weeks after initiating treatment) maximal exercise tests on a 10% inclined treadmill (1 m/s/min increments to fatigue) over a 10-week period (2−3 weeks between tests), with bronchoalveolar lavage (BAL) performed 30 minutes after exercise. Treatment ensued 10 days after the pre-treatment exercise test, with horses receiving a series of five CES or placebo injections 24 hours apart (20 mL intratracheal and 10 mL intravenously), with subsequent weekly injections for 5 weeks thereafter. After CES treatment, both EIPH (RBC in BALF) and inflammation (white blood cell concentration [WBC] in BALF) were significantly diminished by the 4-week posttreatment run, demonstrating 46 ± 12% and 24 ± 11% decreases, respectively (P < 0.05). In contrast, EIPH was elevated significantly at the 4-week time point, and inflammation remained constant in the placebo trial. In conclusion, these preliminary data suggest that therapeutic intervention involving immunomodulation may represent a viable approach to reducing the severity of EIPH. 相似文献
143.
母猪在其生产阶段或哺乳阶段,通常对特定矿物质日粮有其特殊需求,因此正确供给矿物质对确保母猪的生产性能非常重要。 相似文献
144.
Boillée S Yamanaka K Lobsiger CS Copeland NG Jenkins NA Kassiotis G Kollias G Cleveland DW 《Science (New York, N.Y.)》2006,312(5778):1389-1392
Dominant mutations in superoxide dismutase cause amyotrophic lateral sclerosis (ALS), a progressive paralytic disease characterized by loss of motor neurons. With the use of mice carrying a deletable mutant gene, expression within motor neurons was shown to be a primary determinant of disease onset and of an early phase of disease progression. Diminishing the mutant levels in microglia had little effect on the early disease phase but sharply slowed later disease progression. Onset and progression thus represent distinct disease phases defined by mutant action within different cell types to generate non-cell-autonomous killing of motor neurons; these findings validate therapies, including cell replacement, targeted to the non-neuronal cells. 相似文献
145.
Gamete recognition proteins can evolve at astonishing rates and lie at the heart of reproductive isolation and speciation in diverse taxa. However, the source of selection driving this evolution remains unknown. We report on how the sperm bindin genotype influences reproductive success under natural conditions. An interaction between genotype frequency and spawning density determines how sperm bindin genotype influences reproductive success. Common genotypes are selected under sperm-limited conditions, whereas rare genotypes are selected under conditions of intense sperm competition and sexual conflict. Variation in the evolutionary rates of bindin may reflect historic differences in sperm availability. 相似文献
146.
ABSTRACT Resistance is the primary means of control for crown rust of oat (Avena sativa L.), caused by Puccinia coronata f. sp. avenae, and better knowledge of the genetics of resistance will enhance resistance breeding. Disease data were generated in the field and greenhouse for parents and recombinant inbred lines of the Ogle/TAM O-301 (OT) oat mapping population using (i) a new quantitative assay that employs quantitative real-time polymerase chain reaction (q-PCR) to estimate fungal growth in the host, (ii) digital image analysis, and (iii) visual ratings. The objectives of this study were to evaluate each assessment method's ability to map a major gene from cv. Ogle and potential quantitative trait loci (QTL) contributed by Ogle and TAM O-301. All three assessment methods identified the major gene in Ogle, which was mapped to linkage group OT6. The resolution produced by q-PCR, however, enabled more precise mapping of the major gene. Quantitative analysis indicated that 64% of the phenotypic variation was accounted for using q-PCR, whereas 41 and 52% were accounted for using visual and digital assessments, respectively. Data generated by q-PCR permitted identification of QTL on linkage groups OT32, accounting for 6% of the phenotypic variation, and OT2, accounting for 4% of the variation. QTL on both OT32 and OT2 were conferred by TAM O-301, one of which (OT2) was indiscernible using data from the visual and digital assessments. The new method of precisely phenotyping crown rust resistance provided a more accurate and thorough means of dissecting resistance in the OT mapping population. Similar methods could be developed and applied to other important cereal rust diseases. 相似文献
147.
Robertson-Hoyt LA Betrán J Payne GA White DG Isakeit T Maragos CM Molnár TL Holland JB 《Phytopathology》2007,97(3):311-317
ABSTRACT Fusarium verticillioides, F. proliferatum, and Aspergillus flavus cause ear rots of maize and contaminate the grain with mycotoxins (fumonisin or aflatoxin). The objective of this study was to investigate the relationships between resistance to Fusarium and Aspergillus ear rots and fumonisin and aflatoxin contamination. Based on a previous study of 143 recombinant inbred lines from the cross NC300 x B104, 24 lines with the highest and 24 lines with the lowest mean fumonisin concentration were selected for further evaluation. Paired plots of each line were inoculated with F. verticillioides and F. proliferatum or with A. flavus in replicated trials in 2004 and 2005 in Clayton, NC, and College Station, TX. The low-fumonisin group had significantly lower levels of fumonisin, aflatoxin, and Fusarium and Aspergillus ear rots. Across year-location environments, all four traits were significantly correlated; the genotypic correlation (r(G)) ranged from r(G) = 0.88 (aflatoxin and Aspergillus ear rot) to r(G) = 0.99 (Fusarium and Aspergillus ear rots). Quantitative trait loci (QTLs) were identified and their effects estimated. Two QTLs affected both toxin concentrations, one QTL affected both ear rots, and one QTL affected Aspergillus and Fusarium rots and fumonisin. These results suggest that at least some of the genes involved in resistance to ear rots and mycotoxin contamination are identical or genetically linked. 相似文献
148.
The breeding of new sweet potato varieties is a highly inefficient process, confounded by incompatibility, poor fertility,
open-pollination and hexaploidy. Upwards of 12–20 lines are combined in open pollinated nurseries based on horticulturally
important characteristics. After several years of selection most progeny can be traced back to just 3 or 4 maternal lines.
A method that would identify the paternal parent of superior progeny would enable breeders to combine parents that exhibit
superior combining ability in more efficient, smaller nurseries. The objective of this work is to explore by means of computer
simulation the application of genealogy reconstruction techniques on hexaploid individuals based on co-dominant marker data.
The progeny obtained from each female parent is categorically assigned to each male with non-zero exclusion probability based
on its paternity likelihood. Computer simulations show that even with polysomic segregation types, it is possible to discriminate
between putative parents with few errors or mis-assignments. The number of loci scored for a 10 parent population should not
be less than 20 in the case of 3 alleles per locus, and no more than 10 loci for a five allele model. An increment in the
number of alleles or loci increases the discriminatory power with the number of alleles yielding a far more important effect
than the number of loci. This study also demonstrates the feasibility of using simulations to determine the minimum requirements,
i.e. number of loci to be genotyped, for unambiguous parentage allocation in polyploids.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
149.
Seed dormancy mechanisms in warm season grass species 总被引:6,自引:0,他引:6
Embryogenic protoplasts of Dancy tangerine (Citrus reticulata Blanco) were X-ray irradiated at three doses and electrofused with iodoacetic acid-treated embryogenic protoplasts of Page
tangelo (C. reticulata Blanco × C. paradisi Macf.). Shoots could regenerate only from the fusion combination with the lowest irradiation dose, but were recalcitrant
to rooting. In vitro grafting was applied to obtain complete plants. Chromosome examination showed that the plants contained mainly diploid and
aneuploid cells, together with few tetraploid cells, indicating that they were mixoploids. Random amplified polymorphic DNA
analyses with 10-mer arbitrary primers confirmed the plants as true somatic hybrids. This is the first report on regeneration
of mixoploid hybrid plants via protoplast asymmetric fusion in Citrus. Negative effects of ionizing irradiation on regeneration of embryoids and plantlets and possible agronomic interest of the
mixoploid plants are also discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
150.
不同时期追施肥水对冬小麦根系、根系活性和植株性状的影响 总被引:18,自引:3,他引:18
冬前至孕穗期不同生育期追施肥水,对根系的影响不同。追施肥水可提高根系活性,早施,根系活性最大值出现得早,后期根系活性降低得也快。开花、灌浆期的根系活性均以拔节、孕穗肥水为高。根系活性对叶片衰老速度有明显的影响,与上部干重、产量及产量性状存在着密切的关系。 相似文献