Exencephaly in chicken‐pheasant hybrids

1. The incidence of exencephaly was investigated in chicken‐pheasant hybrids.

2. With the pheasant as sire, fertility was 7.lb6%, but with the fowl it was 19.lb5%; hatchability of fertile eggs, however, was similar, 42% and 41% respectively.

3. Only one exencephalic hybrid was obtained when the sire was the pheasant but 70 were noted when the fowl was sire. The incidence of this malformation in hatched chicks was 12 times more in crosses with the fowl as sire than in the reverse cross.

4. The frequency of exencephaly was greater with Rhode Island Red, Barred Leghorn or White Leghorn roosters than with Columbian Rock roosters.     

Responses of immune and nonimmune adult rhesus macaques (Macaca mulatta) to adenovirus SV-20.

Adenovirus SV-20 (ASV-20) was inoculated subcutaneously into adult rhesus macaques (Macaca mulatta), and various immunologic parameters were studied. Similar changes were observed in macaques that had anti-ASV-20 serum-neutralizing antibodies prior to inoculation and in those lacking detectable antibodies. There were absolute decreases in numbers of peripheral blood lymphocytes (PBL), erythrocyte-rosetting lymphocytes, complement-receptor lymphocytes, and Fc-receptor lymphocytes. These changes were most significant (P less than 0.05) on postinoculation days (PID) 3 and 7. Mitogenic responsiveness to concanavalin A, phytohemagglutinin, and pokeweed mitogen in cultured PBL from immune and nonimmune macaques was depressed on PID 3, 7, and 14. Ultraviolet-inactivated ASV-20 caused moderate suppression of phytohemagglutinin-induced mitogenesis when viral particles and lectin were added simultaneously to PBL cultures. Plasma cortisol (hydrocortisone) values were not significantly altered following inoculation of ASV-20. High titers of anti-ASV-20 antibody developed by PID 7 in nonimmune macaques, and previously immune macaques showed a booster effect in the same time period. Antibody titers were still increased 120 days after inoculation. There was no clinical evidence of an adverse effect of ASV-20 infection in these macaques.