Protein synthesis: its control in erythropoiesis

Erythropoiesis in the fetal mouse provides a model to study several important aspects of the regulation of cell differentiation and differentiated protein synthesis. Changes in the patterns of hemoglobins formed during fetal and postfetal development are shown to be associated with the substitution of the liver erythroid cell line. In the course of differentiation of yolk sac erythroid cells there are at least two classes of proteins distinguishable with respect to dependence on continued RNA formatoin. The bulk of nuclear proteins, "nondifferentiated" proteins, appear to be dependent on relatively short-lived messenger RNA while synthesis of differentiated proteins, the hemoglobins, proceeds on relatively stable molecules of messenger RNA. Hemoglobin formation occurs in those cells which are actively synthesizing DNA and dividing. On the average, two to three cell divisions may occur after the formation and stabilization of the messenger RNA for globin. Yolk sac erythropoiesis, at least from day 10 of gestation, is unresponsive to erythropoietin. By comparison, in fetal liver erythropoiesis, the hormone, erythropoietin, acts selectively on the most immature erythroid cell precursor to induce differentiation, cell replication, and hemoglobin formation. The erythropoietin responsive cell in the liver is apparently differentiated from the progenitor, pluripotential stem cell and committed to erythroblast formation and hemoglobin synthesis on exposure to the hormone. The initial effects of erythropoietin on macromolecular synthesis are to stimulate RNA synthesis, which temporally is followed by cell replication and the increase in hemoglobin formation. During liver erythropoiesis, there appears to be a transition from hemoglobin synthesis dependent on RNA formation to hemoglobin synthesis directed by relatively stable messenger RNA.     

Root diseases, weeds, and nematodes with poultry litter and conservation tillage in a sweet corn–snap bean double crop

The effect of continuous applications of poultry litter on root diseases, nematodes, and weeds with different tillage practices in vegetable production in Georgia was investigated. Litter treatments (noncomposted and composted broiler or breeder litter) applied 3–5 weeks before planting were compared with no litter. In sweet corn and snap bean there were differences among years, and tillage and litter treatments had a variable effect on yield, post-emergence damping-off and plant stand. Crown and brace root rot in corn induced by Rhizoctonia solani AG 2-2 increased after 3 years conservation tillage, but there were no differences among litter treatments. Population densities of R. solani AG-4 were increased by conservation tillage before planting snap bean the first year but not in later years, while litter treatments had no effect. After 3 years, populations of Pythium spp., Fusarium solani, and Fusarium spp. did not differ among treatments, but noncomposted broiler litter increased populations of saprophytic fungi in soil compared with no litter. Population densities of stylet-bearing nematodes were low and usually not different among treatments. After 3 years of sweet corn populations of Meloidogyne incognita increased with conventional tillage compared with conservation tillage, populations of Paratrichodorus christiei were reduced by litter treatments and populations of Helicotylenchus dihystera were reduced by noncomposted broiler litter compared with no litter. Weed infestation increased in both tillage systems with time, but in snap bean there was a greater infestation in conservation tillage than in conventional tillage. Litter treatments had little effect on weed infestation.     

Modeling the Effect of pH and Salinity on Biogeochemical Reactions and Metal Behavior in Sediment

A mathematical model is developed to investigate the effect of pH and salinity fluctuation on biogeochemical reactions and metals' behavior in sediments. The model includes one-dimensional vertical advective and diffusive transport of species, serial reductions of electron acceptors, and precipitation/dissolution of species, acid–base chemistry, and metal sorption to sediments. The model was tested using data obtained from laboratory microcosm experiments which exposed metal (Cd, Zn) contaminated sediment to alternating fresh and salty overlying water. The model successfully reproduces the contrasting metal's release behavior and the vertical profiles of pH, Cl^?, SO₄ ^2?, Mn and Fe in porewater and the acid volatile sulfides (AVS) and simultaneously extracted metals (SEM) in sediments. The model showed that FeOOH_(s) was the dominant sorption phase controlling the solubility of the metals at the surficial sediments while AVS controlled the solubility of the metals in anoxic sediments. The model also showed that the release of the metals to overlying water was controlled by the oxidation of metal sulfides in a very thin layer of oxic sediments (2–3 mm). The proposed model can be useful in managing metal contaminated sediments where pH and salinity are fluctuating by assessing the underlying biogeochemical processes and metals' behavior.     

"Green" composites from renewable resources: preparation of epoxidized soybean oil and flax fiber composites

In recent years there has been considerable interest in using natural plant fibers as reinforcements for plastics. The motivation includes cost, performance enhancement, weight reduction, and environment concerns. High performance flax fiber could potentially substitute for glass or carbon fibers as reinforcements for plastics. This study reports the "green" composites obtained from a mixture of epoxidized soybean oil and epoxy resin, 1,1,1-tris(p-hydroxyphenyl)ethane triglycidyl ether (THPE-GE), reinforced with flax fiber. The compression molding method is used for making the composites. Curing agents triethylenetetramine and diethylenetriamine provide better physical properties of the composites than Jeffamine agents D-230 and EDR-148. Both the flexural modulus and the tensile modulus of the composites increase as the amount of THPE-GE increases. The flexural modulus increased at a fiber content of <10 wt %, but there is a decrease beyond 10 wt %. The tensile modulus increases with fiber content until a maximum at 13.5 wt %, and then it decreases. The flax fiber length affected the mechanical properties of the composites: the longer the fiber length, the better are the mechanical properties observed.     

Effect of added caffeic acid and tyrosol on the fatty acid and volatile profiles of camellia oil following heating

Camellia oil is widely used in some parts of the world partly because of its high oxidative stability. The effect of heating a refined camellia oil for 1 h at 120 degrees C or 2 h at 170 degrees C with exogenous antioxidant, namely, caffeic acid and tyrosol, was studied. Parameters used to assess the effect of heating were peroxide and K values, volatile formation, and fatty acid profile. Of these, volatile formation was the most sensitive index of change as seen in the number of volatiles and the total area count of volatiles in gas chromatograms. Hexanal was generally the dominant volatile in treated and untreated samples with a concentration of 2.13 and 5.34 mg kg(-1) in untreated oils heated at 120 and 170 degrees C, respectively. The hexanal content was significantly reduced in heated oils to which tyrosol and/or caffeic acid had been added. Using volatile formation as an index of oxidation, tyrosol was the more effective antioxidant of these compounds. This is contradictory to generally accepted antioxidant structure-activity relationships. Changes in fatty acid profiles after heating for up to 24 h at 180 degrees C were not significant.     

Frictional afterslip following the 2005 Nias-Simeulue earthquake, Sumatra

Continuously recording Global Positioning System stations near the 28 March 2005 rupture of the Sunda megathrust [moment magnitude (Mw) 8.7] show that the earthquake triggered aseismic frictional afterslip on the subduction megathrust, with a major fraction of this slip in the up-dip direction from the main rupture. Eleven months after the main shock, afterslip continues at rates several times the average interseismic rate, resulting in deformation equivalent to at least a M(w) 8.2 earthquake. In general, along-strike variations in frictional behavior appear to persist over multiple earthquake cycles. Aftershocks cluster along the boundary between the region of coseismic slip and the up-dip creeping zone. We observe that the cumulative number of aftershocks increases linearly with postseismic displacements; this finding suggests that the temporal evolution of aftershocks is governed by afterslip.     

荔枝年生长周期内N、P、K营养动态规律与施肥管理体系

通过3年大田试验探索荔枝年生长周期内N、P、K营养规律,验证由高氮、高磷钾、高钾复合肥组成的荔枝平衡施肥管理措施的适用性。结果表明,荔枝年生长周期内,收获期N、P、K营养水平降至最低,随后因施肥而迅速恢复,在秋梢老熟到花芽分化期达年周期内最大值,此后随着开花结果N、P、K营养水平逐渐下降,至新的收获期又降至最低水平。秋梢和果枝的N、P、K水平因生长期的不同而明显变化,然而肥料用量(3～5kg/树)和N∶P∶K比例对树体N、P、K水平的影响较小。每个生育期结果母枝的N、P、K含量依次为N>K>P。收获期到开花前是树体营养恢复阶段,开花到果实生长发育期是消耗体内贮藏态N、P、K的过程。在荔枝年生长周期内,分别在收获期、秋梢老熟期和坐果期施用高氮复合肥、高磷钾复合肥和高钾复合肥能保障年周期内荔枝树体N、P、K营养水平处于最佳状态。     

Assessing the benefits of the marine protected area of Punta Coles,southern Peru (south-east Pacific) on productivity of conspicuous benthic species using pre-image population analysis

1. The coastal marine protected ecosystem at Punta Coles (as a part of the Reserva Nacional de Islas, Islotes y Puntas Guaneras) is located in a highly exploited area in southern Peru and contains several commercial, bioengineering and other benthic species.
2. Pre-image population analysis was used to estimate the transient values of intrinsic growth rate (r) based on biomass and density time series as a proxy of productivity to assess the effectiveness of protection.
3. The outcomes obtained showed that the gastropods Concholepas concholepas, Fissurella latimartiginata and the tunicate Purya chilensis showed a reduction of productivity during recent years, whilst the sea urchin Loxechinus albus, the barnacle Balanus laevis and the bivalve Semimytilus algosus showed an opposite pattern. Several species exhibited a chaotic dynamic, coinciding with the highest productivity values. In 2016 and 2017, several species exhibited a reduction of their abundance which could be delayed responses explained by changes in oceanographic conditions (reduction of coastal upwelling), La Niña event and illegal harvest.
4. The above notwithstanding, the results suggest that effective protection of benthic species in the protected ecosystem of Punta Coles have been partially accomplished. Therefore, this work could be considered a baseline study, permitting the subsequent monitoring of productivity of the main species inhabiting the coastal area of Punta Coles.
5. Pre-image population analysis could be used as a complimentary analytical tool since it enables the evaluation of transient population parameters (e.g. productivity) thus aiding population management, conservation and monitoring decisions.

     

Effects of Rough Rice Storage Conditions on the Amylograph and Cooking Properties of Medium-Grain Rice cv. Bengal

Rough rice (cv. Bengal) was stored at four moisture contents (8.8, 10.7, 12.9, and 13.6% MC) and three temperatures (3, 20, and 37°C) for up to six months. The amylograph overall paste viscosity of the milled rice increased during storage. This increase was most apparent in all samples stored at 37°C. For rice stored at 20 and 37°C at all MC levels, a 30–50% increase in peak viscosity (PV) was observed during the first three months of storage. PV subsequently leveled off for rice stored at 12.9 and 13.6% MC but declined for samples stored at 8.8 and 10.7% MC. The final viscosities also increased during storage. The water-absorption ratio of the samples during cooking in excess water increased by an average of 15% over six months of storage. The amylograph and cooking properties were significantly affected (P < 0.05) by rough rice storage duration, temperature, MC, and their respective interactions.