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181.
Atlantic sturgeon (Acipenser oxyrhynchus), which are bottom dwelling and migratory fish, experience environmental hypoxia in their natural environment. Atlantic sturgeon,
acclimated to either 5 or 15°C, were subjected to a 1 h severe (<10 mm Hg) hypoxia challenge in order to document their physiological
responses. We measured hematological parameters, including O2 transport (hemoglobin, hematocrit), ionic (chloride, osmolality), and metabolic (glucose, lactate) variables under normoxic
conditions (~160 mm Hg), immediately following a 1 h exposure to hypoxic water, and following a further 2 h of recovery from
this challenge in normoxic water. In a second experiment, we assessed the opercular beat frequency before, during, and after
hypoxic exposure. Hemoglobin concentrations and hematocrit were significantly different between fish held at 5°C vs. 15°C
and also significantly different between normoxia prior to hypoxia and following recovery. Plasma lactate concentrations increased
following hypoxia at both temperatures, indicative of an increase in anaerobic metabolism. In contrast, a significant increase
in plasma glucose concentrations in response to hypoxia only occurred at 5°C, suggesting different fuel demands under different
temperatures. Changes in opercular beat frequency (OBF) were dependent on temperature. At 5°C, OBF increased upon exposure
to hypoxia, but returned to pre-exposure levels within 35 min for the remainder of the experiment. During hypoxia at 15°C,
OBF increased very briefly, but then rapidly (within 20 min) decreased to levels below control values. Following a return
to normoxia, OBF quickly increased to control levels. Overall, these findings suggest that Atlantic sturgeons are relatively
tolerant to short-term and severe hypoxic stress, and the strategies for hypoxia tolerance may be temperature dependent. 相似文献
182.
Jeanne B. Wexler Vernon P. Scholey Robert J. Olson Daniel Margulies Akio Nakazawa Jenny M. Suter 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):327-353
A land-based culture facility for research on yellowfin tuna, Thunnus albacares, was developed at the Achotines Laboratory in the Republic of Panama. Six concrete tanks, and seawater and life support systems were built to maintain a yellowfin broodstock. On average, 50% of the yellowfin caught survived capture and handling, and approximately 30% became broodstock in Tank 1 (17 m diameter, 6 m depth) or Tank 2 (8.5 m diameter, 3 m depth). Each fish was tagged with a microchip implant tag, then weighed, measured, and injected with oxytetracycline (OTC) prior to stocking. Daily rations of primarily market squid, Loligo opalescens, and Pacific thread herring, Opisthonema spp., were regulated based on the feeding activity and energy requirements of the fish. Feeding activity of the broodstock decreased when the water temperatures decreased, and the fish ate decreasing daily rations and increasing calories with increasing size. Spawning occurred in both tanks within 6–8 months of capture. Spawning first occurred in Tank 1 when 24 females ranged in size from 6 to 16 kg and 65 to 93 cm fork length (FL). Spawning was intermittent during the first 2 months and occurred near daily thereafter. Tank size appeared to affect survival rates, the types of mortalities that occurred, and the growth of the fish. Survival rates after 1 year in captivity were higher, and the fish were larger, on average, in Tank 1 than in Tank 2. Most of the mortalities in Tank 1 were the result of wall strikes, which occurred more frequently after the fish reached their highest density of 0.64 kg m−3 and sizes greater than 96 cm FL and 19 kg. Non-linear growth models were fitted to the initial stocking sizes and final sizes of fish that died or were removed from Tank 1 during 1996–1999. Estimated growth rates in length (11–48 cm year−1) for fish between 51 and 150 cm FL decreased with increasing length. Estimated growth rates in weight ranged from 9 to 19 kg year−1 for fish less than 19 kg and 20–23 kg year−1 for fish greater than 19 kg. The results of this work demonstrate that the stable environment of a land-based culture facility may be the preferred system for long-term maintenance of a yellowfin broodstock. 相似文献
183.
Visualisation of standardized life-history patterns 总被引:1,自引:0,他引:1
The life‐history patterns of fish and invertebrate species are complex. But much of this complexity can be captured in simple diagrams of coastal transects, where juveniles usually occur in larger numbers in shallow waters, while adults generally inhabit deeper, offshore waters. Iconographic representations of generalized life‐history patterns and depth profiles, with specific key life‐history parameters can capture much of these standardized patterns, including spawning areas, nursery/juvenile distributions, adult distributions and spawning migrations. Several examples presented here from a wide range of habitats and ecosystems (temperate and tropical waters, demersal, deep water, pelagic and coral reefs), including an example of different stocks of the same species, illustrate some general patterns with regard to water depth and distance from shore. The present approach should be viewed as a first step towards obtaining standardized patterns about key life‐history parameters, and will hopefully lead to incorporation into management of life‐history interconnectivity between different fishery sectors or gears. This may contribute to sustainable, ecosystem‐based approaches to management by informing policy options when faced with decisions to rationalize overcapitalized fisheries. 相似文献
184.
Alberto J.P. Nunes Marcelo V.C. S Francisco Felipe Andriola-Neto Daniel Lemos 《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):244-254
Nine commercial feeding attractants and stimulants for Litopenaeus vannamei were evaluated by observation of behavioral responses in animals allotted in one Y-maze aquarium apparatus. In the validation phase, fishmeal–Brazilian origin (FMBO); fishmeal–Peruvian origin (FMPO); blood meal (BM), meat and bone meal (MBM), squid meal (SM), fish oil (FO) and fish solubles (FS) were evaluated. There was also a control without stimulatory raw material. The tested materials were included at 3% in neutral gelatin pellets (wet basis). In each behavioral observation, two different ingredients were offered at the same time in equal quantities, being allotted in the end of each chamber's arm. In Phase II after system validation, the following commercial attractants were tested: 80% crude protein (CP) vegetable dried biomass (VDB80), 68% CP vegetable dried biomass + glutamate + betaine (VDB68), complex of amino acids (alanine, valine, glycine, proline, serine, histidine, glutamic acid, tyrosine and betaine) with enzymatically digested bivalve mollusk (CAA), condensed fish soluble protein (CFSP), squid liver meal (SLM), betaine (Bet), dried fish solubles–low biogenic amines (DFSLB), dried fish solubles–high biogenic amines (DFSHB) and whole squid protein hydrolysate (WSPH). Attractants were used at a 3% level wet basis with neutral gelatin, without any additional ingredient source available. The best four commercial attractants from this phase (CAA, CFSP, SLM and WSPH) were compared under 0.5% and 1.0% levels. In Phase I of the study, a higher percentage of choices were observed for FMPO and FMBO. BM and FO were the least chosen ingredients. In Phase II, the worst results were observed for Bet, DFSHB and, mainly, for VDB80 and VDB68. When two-by-two comparisons were performed, results suggested that CFSP and CAA were the best commercial attractants tested. In the last phase, both CFSP and CAA at 1.0% level were significantly more chosen by shrimp than CFSP (0.5%), SLM (0.5 or 1.0%) or WSPH (0.5 or 1.0%). At both 0.5% and 1.0% levels, shrimp spent a similar amount of time feeding on CFSP and CAA. However, only CAA was statistically better than SLM and WSPH together. Further work is required to better elucidate the chemical drivers of chemostimulation for L. vannamei for each one of the attractants tested. 相似文献
185.
Emily F. Pomeranz Daniel J. Decker William F. Siemer Arthur Kirsch Jeremy Hurst James Farquhar 《Human Dimensions of Wildlife》2014,19(5):448-457
The trend in wildlife management over the last two decades has been to develop locally based approaches for responsiveness to local conditions, but some state wildlife agencies are finding the amount of staff time required to service this approach prohibitive. Although local engagement strategies have been lauded as assuring that public trust obligations of state government to citizens are met, we can expect that states with a local focus as their operational level of stakeholder engagement may opt to change their approach to reflect their resource limitations. We argue for comprehensive regional level effort to understand stakeholders augmented with local engagement processes where needed to deal with special circumstances in smaller areas within a region. Such an approach can be anticipated to have implications for stakeholder engagement and human dimensions research needs, which we discuss in the context of public trust resource administration and good governance of wildlife resources. 相似文献
186.
Daniel Elad 《Veterinary journal (London, England : 1997)》2014,199(3):319-323
The history of recorded cases of anthrax in human beings and animals from 1909 to 2012 in the area between the Jordan River and the Mediterranean Sea is reviewed. The disease was endemic until the middle of the 20th century, but the incidence decreased thereafter, with only sporadic cases from the 1980s onwards. Human cases have not been diagnosed in the region since 1984 and the number of episodes of animal disease has reduced to less than one per year. This decline is mostly due to the disruption of the infective cycle by improved veterinary control, including vaccination, treatment and outbreak management. A policy of reactive vaccination for 10 years of affected herds and herds grazing in their proximity has been applied. No new outbreaks have been observed in such herds after the cessation of vaccination, despite continued grazing on the same sites, so it is assumed that spore survival in such areas is shorter than 10 years. This is independent of the soil composition, which is calcareous throughout most of the relevant area. However, reemergence of anthrax, even after decades, has occurred following disturbance and heavy rainfall. 相似文献
187.
Daniel M. Parker Brendan M. Whittington-Jones Richard T. F. Bernard Harriet T. Davies-Mostert 《Human Dimensions of Wildlife》2014,19(6):512-522
Subsistence pastoralists often view large carnivores negatively given that any loss of livestock is likely to represent a significant economic cost. Consequently, large carnivore conservation initiatives in rural landscapes should incorporate appropriate human–carnivore conflict mitigation strategies. In this study, structured interviews with local residents (n = 247) were used for determining attitudes toward African wild dogs (Lycaon pictus) among rural communities neighboring two protected areas in KwaZulu-Natal, South Africa. Contrary to expectations, the majority of respondents (58%) were generally positive toward wild dogs. This was probably due to a lack of livestock depredation by wild dogs and relatively high levels of formal education among respondents. Respondents with greater knowledge of wild dogs and conflict mitigation had more positive attitudes, and most were aware of effective husbandry techniques. The positive attitudes observed in these rural communities may benefit wild dog dispersal from natal home ranges and the future conservation of this endangered carnivore. 相似文献
188.
189.
Manuel Martin‐Flores Daniel M Sakai Luis Campoy RD Gleed 《Veterinary anaesthesia and analgesia》2014,41(3):269-277
ObjectiveTo evaluate if return of spontaneous ventilation to pre-relaxation values indicates complete recovery from neuromuscular blockade.Study designProspective, with each individual acting as its own control.AnimalsTen healthy adult female Beagle dogs weighing 6.2–9.4 kg.MethodsDogs were anesthetized with propofol, dexemedetomidine and isoflurane. Spontaneous ventilation was assessed by measuring end-tidal CO2, expired tidal volume, peak inspiratory flow, respiratory rate and minute ventilation. Vecuronium 25 μg kg?1 IV was administered and neuromuscular block was evaluated by measuring the train-of-four (TOF) ratio with acceleromyography in the hind limb. During spontaneous recovery from neuromuscular block, the TOF ratio when each ventilatory variable returned to baseline was recorded.ResultsThis dose of vecuronium produced moderate neuromuscular block in all dogs, with TOF ratio values of 0–18% at maximal block. Expired tidal volume, peak inspiratory flow and minute ventilation returned to pre-relaxation values when the median TOF ratio was ≤ 20%. The median TOF ratio was 42% when the end-tidal CO2 returned to pre-relaxation values.Conclusions and clinical relevanceSignificant residual neuromuscular block could be measured at the hind limb with acceleromyography when ventilation had spontaneously returned to pre-vecuronium values. Monitoring spontaneous ventilation, including end-tidal CO2, expired tidal volume, peak inspiratory flow or minute ventilation cannot be used as a surrogate for objective neuromuscular monitoring, and this practice may increase the risk of postoperative residual paralysis. 相似文献
190.
John M David Sandra Duarte Vogel Kimberly Longo Daniel Sanchez Gregory Lawson 《Veterinary anaesthesia and analgesia》2014,41(6):654-659
ObjectiveTo investigate a topical local anesthesia technique as a means to prevent and/or diminish pain in mice in a laboratory setting associated with tail vein injections performed by personnel in training.Study designProspective, randomized experimental trial.AnimalsThirty six adult female, 23–28 g CD-1 mice from an in-house training colony. They were acclimated to routine training and handling classes.MethodsEutectic mixture of local anesthetics (EMLA) cream (2.5% lidocaine/2.5% prilocaine) or a bland ointment control (n = 18) was applied on the tail prior to intravenous injection. The injections were performed by novices, who had never attempted the procedure, and experienced personnel. All participants were blinded to treatment groups. Three injection attempts were allowed per animal. The mice were observed and scored by blinded evaluators for behavioral and physiological changes, including respiratory rate, vocalization, tail flick, and escape behaviors, during and after the injection.ResultsThis study demonstrates that aversive behaviors induced by lateral tail vein injection were not changed by the preemptive application of EMLA cream. The aversive behaviors associated with lateral tail vein injection were significantly affected by the number of injection attempts and the individual's experience level.Conclusions and clinical relevanceTopical EMLA cream did not reduce signs of aversive reaction to tail vein injection and thus we did not find support for its use in mouse training programs for tail vein injections. 相似文献