贵州黔西南州野生三叶青种质资源调查分析

目的:黔西南州分布有大量的名贵中药材,但很多资源分布情况尚不清楚,如三叶青,导致对野生药材的研究滞后于其他省份.故对黔西南州野生三叶青进行调查分析,旨在鉴定各县市三叶青的分布情况及各地三叶青之间的性状差异.方法:通过在州内各县市的调查,收集一定数量的各地野生三叶青资源,对比分析各地野生三叶青的性状特征.结果:黔西南州分布有大量野生三叶青资源,且为不同的种质类型.结论:黔西南野生三叶青资源分布较广泛,分为茎藤褐紫色和茎藤青绿色两大类,具有巨大的研究价值.     

青海半地下式日光节能温室结构及建造

介绍了半地下式日光节能温室的结构,根据青海省气候特点,进行优化设计,提出了半地下日光节能温室墙体厚度、后屋面、畦面下切1.4-1.5m等结构设计参数,以及温室建设的技术及流程。     

柞蚕雄蛾油中α-亚麻酸对小鼠血脂代谢的影响

为了研究柞蚕雄蛾油中α-亚麻酸对高脂小鼠血脂代谢的影响,给高脂小鼠以不同剂量的α-亚麻酸灌胃,并每周称体重,4周后测定小鼠肝系数及血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)。结果表明:与摄食高脂饲料的小鼠相比,柞蚕雄蛾油处理组中的α-亚麻酸可使小鼠血清TC下降22.48%~29.04%,TG下降25.53%~46.38%,LDL-C下降30.17%~45.70%,HDL-C升高11.18%~26.97%,并能明显降低小鼠体重及肝系数。可见,α-亚麻酸能够较好的调节小鼠血脂代谢,明显降低摄食高脂饲料小鼠的血脂水平。     

西方盲走螨防治苹果树叶螨的研究

通过室内个体饲养观察,明确西方盲走螨捕食山楂叶螨和李始叶螨生长发育情况良好。1983～1985年,兰州田间试验表明:5月下旬到6月中旬,根据苹果树的不同树龄和叶螨的虫口基数,以1:36～64的益、害比,每株释放西方盲走螨雌成螨350～2,750头,经过45～60天,释放树上山楂叶螨和李始叶螨的种群数量发展缓慢,渐趋衰亡,达到完全控制。释放西方盲走螨不仅当年控制李始叶螨为害,可减少用药3～4次;其效果可持续两年。     

直播稻田杂草的生态特点和控制技术研究

近年来,随着直播稻种植面积不断扩大和栽培技术不断改革,田间草相也随生态条件的不同而发生了变化。水稻前期干、湿栽培,十分有利老草复活和新草的萌发生长。直播稻田杂草危害已成为阻碍直播稻优质高产和大面积推广应用的主要障碍。为了明确直播稻田杂草的生态特点和杂草萌发规律,科学制定防除策略,积极探索直播稻田杂草的化除技术,安全、有效、经济地将杂草危害损失控制在最低限度,我们于2003年开展了直播稻田杂草的生态特点和防除技术研究,初步摸清直播稻田出草规律,得出化除配套技术,并进行了大面积推广应用,取得了显著的灭草、增产效果…     

75%磺酰唑草酮干悬浮剂芽前防除蔗田杂草研究

75%磺酰唑草酮干悬浮剂分别以30、40、50、60g/667m²用量,芽前土壤处理防除蔗田马唐、牛筋草、狗尾草、香附子、胜红蓟、铜锤、苋菜等杂草。试验结果表明,药后30、60d株防效分别达73.1%～100.0%、66.7%～100.0%,总草防效分别为90.7%～98.7%、83.7%～97.6%;药后60d,鲜重防效达68.4%～100.0%,总草防效为87.8%～99.4%,对一年生单双子叶杂草表现良好的广谱性。药后30、60d总草株数和鲜重防效显著优于38%莠去津悬浮剂250g/667m²的效果。75%磺酰唑草酮干悬浮剂60g/667m²对甘蔗出苗和生长有一定的影响,但与其它处理无显著差异;50g/667m²增产率最高为12.79%;30g/667m²增产率最低,但也增产7.06%;其它处理增产均在9.05%以上,显著高于莠去津和人工除草。     

In vitro inhibitory effect of CD8+ cells from patients with aplastic anemia on normal CFU-Mk growth is blocked by cimetidine

AIM: To investigate the effect of CD8+ cells from aplastic anemia (AA) patients and its histamine type II (H2) receptors on the growth of normal CFU-Mk. METHODS: The effects of CD8+ cells and/or cimetidine, on normal human CFU-Mk growth were studied by using CFU-Mk assay. RESULTS: The CD8+ cells from the perpheral blood of AA patients significantly suppressed the growth of normal allogeneic CFU-Mk. This inhibitory effect was blocked by cimetidine at concentration of 1.0×10-5 mol/L. 1.0×10-5 mol/L cimitidine alone didn't inhibit the growth of normal CFU-Mk. CONCLUSION: H2 receptor antagonist cimitidine abolishes the suppressive effect of AA patients CD8+ cells on the growth of normal CFU-Mk.     

Establishment and biological characteristics of a new human endometrial carcinoma

AIM: To establish a new human endometial carcinoma cell line for basic and clinical study. METHODS: The minced tumor tissues were inoculated subcutaneously into the back of nude mice. After forming solid tumor, the mice were killed and the transplant was taken out for primery culture. RESULTS: The established cell line was maintained by serial passages, named EAC. EAC cell line grew rapidly,steadily, and it had similar microscopic morphology to adenocarcinoma cell. Chromosome analysis reveal EAC chromosome counts ranging in hyperdiploid. The expression of ER, PR, P53, MDM2 were negative by SP immunohistochemistry. Tumorigenicity of EAC in nude mice was 100%. CONCLUSION: A new human endometrial carcinoma, EAC, is established successfully, which is helpful for the basic and clinical study of endometrial carcinoma.     

B allele in Iα1 hs1,2 VNTR region is associated with IgA nephropathy

AIM: To investigate the relationships between Iα1 hs1,2 VNTR polymorphism and IgA nephropathy. METHODS: Four hundred and ninteen patients with IgA nephropathy and their first-degree relatives were recruited. Two hundred and one sex and age-matched normal Chinese Han volunteers were also recruited as controls. After extracting genomic DNA, the VNTR genotypes of Iα1 hs1,2 region were determined by PCR and electrophoresis, and the results were analyzed by transmission disequilibrium test (TDT) and haplotype relative risk (HRR) in the families, and Chi-Square test in the case-control analysis. RESULTS: ① TDT analyses showed that B allele of the Iα1 hs1,2 VNTR region was significantly more transmitted from heterozygous parents to patients than expected (101 Trios, χ2=6.818, P＜0.01), extended TDT produced the same results (164 families, χ2=7.583, P＜0.01). ② Consistent with the TDT results, HRR also showed that B allele was over-transmitted to patients (P＜0.05, χ2=4.122, HRR=1.180), and the BB genotype conferred a higher risk of developing the disease (P＜0.05, χ2=4.411, OR=1.538). ③ The case-control study indicated that the B allele had a higher frequency in the IgA nephropathy group (χ2=6.968, P＜0.05). CONCLUSION: B allele in Iα1 hs1,2 VNTR region is associated with susceptibility to IgA nephropathy.