稻棉轮作对棉红蜘蛛控制作用的研究

稻棉轮作棉田因轮种水稻使红蜘蛛越冬的土壤和寄主杂草环境受到严重破坏，减少了红蜘蛛的越冬基数及早春活动繁殖场所，推迟了进入棉田为害的时间，并促进棉田中天敌数量的增加。从而有效地控制了轮作棉田红蜘蛛的发生危害，减少了防治棉田红蜘蛛的用药次数。     

甲壳动物保幼激素的研究

近年来，在甲壳动物大颚腺中发现了一种具有保幼激素活性的物质——法呢酸甲酯（MF），它是一昆虫保幼激素Ⅲ（JHⅢ）结构相似的一种倍半萜类化合物，对甲壳动物的生长、发育和繁殖有重要调控作用，而MF的分泌受到眼柄等因子的负调节作用。     

加系双肌臀大白猪不同杂交组合性能比较试验

本试验选择加系双肌臀大白猪、美系杜洛克、丹麦长白三个品种的公母猪,组成5个杂交组合(双公×双母,双公×长母,双公×杜母,杜公×双母),测定了双×双、长×双、双×长、双×杜、杜×双五个组合猪的繁殖性能与肥育性能。结果表明:杜×双组合在日增重(896.05g)、饲料报酬(2.80∶1)、瘦肉率(66.01%)等主要指标上优于其它杂交组合,且繁殖性能居中、胴体品质优良,是加系双肌臀大白猪二元杂交瘦肉猪生产中的最佳杂交父本品种。试验还表明,双肌臀大白猪产仔多(12.08头)、增重快(851.97g)、瘦肉率高(65.24%)、适应性强,值得大力繁殖推广。     

An experimental study of hypercholesterolemia-induced disorder of Oddi's sphincter

AIM:To investigate the functional changes and histopathological basis of Oddi’s sphincter under hypercholesterolemia. METHODS:Twenty - four New Zealand rabbits were equally divided into be groups ran- domly. Experimental groups Ⅰ and Ⅱ fed with cholesterol - added forage for 4 and 8 weeks respectively before sacri- ficed. Images, functions and histopathological characteristics of Oddi’s sphincters of experimental groups were studied and compared with control group by cholangiography, catheter manometry, and quantitative analysis of nitric oxide synthase. RESULTS: The hasal pressures of proximal low - pressure segment of Oddi’s sphincter of hath experimen- tal groups Ⅰand Ⅱ (20.9±6.1 mmHg, 25 .6±9.1 mmHg, respectively) were higher than those of control group sig- nificantly (11.7±2.8 mmHg, P < 0.01, P < 0.01 ). The hasal pressures of distal high - pressure "valve" segment of both experimental pops (138 .4±45. 5 mmHg and 144. 5 ±40 .4 mmHg, respectively) were significantly higher than those of coned group (69.3±9.8 mmHg, P < 0.01 and P<0.01). The gallbladder volUmes of experimental group Ⅰ and Ⅱ (3.4±1.0 mL and 3.9±1.9 mL) were significantly larger than those of control group (2.0 ±1.9 mL, P < 0.05 and P < 0.01 ). Between groups there were no significant differences in nitric oxide synthase con- tents. Enlargement of the cell volume, wavy distortion of the cell membrane, loosening and disarrangement of myofila- ment, uneven in size and distribution of dense body, angulation of longitudinal axis between dense body and myofila- ment, swelling, congregated at one end of nucleoulus and disappearance of cristae of plasmosomes and concertration of chromosome at nucleolus margin were observed in experimental groups under electron-microscope. CONCLUSION: The diastole function of Oddi’s sphincter could be damaged by hyperecholesterolemia, inducing a functional disorder of Oddi’s sphincter with increased resistance in bile excretion. The result is retertion of bile in the gallbladder.     

鸡肝脏和肌肉组织中常山酮残留的ELISA检测

将常山酮进行人工改造,制备了半抗原常山酮琥珀酸衍生物（Hal-suc）.采用N-羟基琥珀酰亚胺活性酯法将半抗原与牛血清白蛋白（BSA）、卵清白蛋白（OVA）偶联,制备免疫原和包被原.动物免疫6次后采血制备抗血清,以间接ELISA法测定血清效价,测得抗血清的最佳工作浓度为1∶102 400.建立了常山酮在鸡肝脏和肌肉组织中检测的ELISA法,该方法在50、100、500 ng/g 的添加浓度水平下,在鸡肝脏和肌肉组织中测得的平均回收率范围分别为74.2%～96.8%和74.3%～90.0%,检测限分别为28和19 ng/g.     

基因型鹅副粘病毒NA-1株全基因组的克隆及特性分析

将本实验室分离保存的NA-1株鹅副粘病毒(GPMV)经SPF鸡胚增殖,收集鸡胚尿囊液进行病毒纯化,提取病毒基因组RNA。参考GenBank已收录的GPMVZJ1株基因组序列,设计了8对特异性引物,RT-PCR法分别特异性的扩增出病毒各基因片段,并将各目的基因片段回收纯化,克隆PGEM-T载体,转化大肠杆菌DH5α,小提质粒选取阳性克隆酶切鉴定并进行序列测定,对测定结果进行拼接得到全长cDNA序列(GenBank登录号:DQ659677)。NA-1株核苷酸比新城疫病毒(NDV)核苷酸多6个碱基,位于1644~1645nt之间,符合NDV核苷酸“六碱基原则”。序列分析结果表明,GPMVNA-1株与各地代表株核苷酸同源性81.2%~91.3%。同时根据各毒株F基因开放阅读框前389个核苷酸序列绘制出系统发育进化树,结果表明GPMNNA-1株与SF02和QY97病毒株同属于基因型,不同于基因IX型NDV的国家标准株F48E9和基因型的LaSota传统弱毒株。     

鸡IFN-γ重组鸡痘病毒对鸡传染性腔上囊炎MB43弱毒疫苗免疫鸡免疫应答的影响

将重组鸡痘病毒（rFPV—IFN-γ）与IBDMB43弱毒疫苗联合接种SPF鸡，研究重组鸡IFN-γ对IBD疫苗免疫效果的影响。结果显示，rFPV—IFN-γ和MB43弱毒疫苗联合免疫组（MB43＋rFPV-IFN-）＂）及rlFN-γ和MB43弱毒疫苗联合免疫组（MB43＋rIFN-γ）在免疫后第2周即可检出ELISA抗体，比MB43疫苗单独免疫组早1周。用IBDVGx株攻击后，MB43＋rFPV-IFN-γ组和MB43＋rIFN-γ组免疫鸡的脾只有个别淋巴细胞核浓缩、核崩解，少数滤泡萎缩变性坏死；胸腺损伤程度轻于MB43疫苗单独免疫组和非免疫对照组。免疫后1周，3个免疫组外周血CD8^＋T淋巴细胞含量均显著高于非免疫对照组，CD4^＋T淋巴细胞含量变化不明显；攻毒后第2周，3个疫苗免疫组CD4^＋、CD8^＋T淋巴细胞含量均显著升高，各组之间CD4^＋、CD8^＋T淋巴细胞含量无明显差异。证实，rFPV—IFN-γ和rIFN-γ可加强疫苗的细胞免疫和体液免疫应答。     

口蹄疫病毒整联蛋白受体的研究进展

从组织分布、各毒株利用率、配体结合区介导感染的能力及β亚基胞质区作用等方面论述了4种整联蛋白αvβ1、αvβ3、αvβ6和αvβ8的研究进展，旨在阐明各整联蛋白决定口蹄疫病毒宿主组织嗜性的作用，并为口蹄疫病毒致病机制的研究提供理论依据。     

Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3′ untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus doris (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (p < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results.     

饲料配方中影子价格的定义及其应用

1 饲料配方中影子价格的定义和意义 在以线性规划为基础的饲料配方模型分析和应用中，对影子价格的表述有两个：一是配方营养约束条件的影子价格；二是原料的影子价格。