数字环境下农业科研档案资源开发与发展路径

当前,农业科学技术研究档案面临数字经济带来的历史性变革.文章基于农业科研档案的内涵价值,探讨数字环境下农业科研档案创新发展的模式与路径.以深化农业科研档案资源价值为导向,分析应用数字技术解决传统农业科研档案数字化数据与现行数字农业科研档案融合管理的实践策略与技术方案.分别分析传统载体数字化数据与现行数字农业科研档案的优...     

减蛋综合征病毒环介导等温扩增检测方法的建立

根据GenBank登录的减蛋综合征病毒(EDSV)六邻体基因的保守区,设计了3对环介导等温基因扩增(LAMP)引物,优化反应体系,并对其灵敏性、特异性进行验证.结果表明:LAMP优化后的反应体系为外引物浓度0.1μm01·L-1,内引物浓度0.8 μmol·L-1,环引物浓度0.4μmol·L-1,Mg2+浓度8.0 ...     

泗水县气象灾害预警平台建设探析

介绍了泗水县气象灾害预警平台的建设与应用情况、配置技术要求以及监测系统站网布设的原则。利用Internet网络、GIS地理信息、蜂窝网络带宽传输、嵌入式文语转换、LED显示控制等技术,按照山洪地质灾害预警平台配置技术要求以及监测系统站网布设原则,建设成了泗水县集无线文转语应急广播、电话应急广播、无线LED显示屏发布于一体的灾害预警平台,实现了各级气象灾害预警平台与相关部门的基础信息采集系统对接,建立社会经济和自然地理基础资料数据库、气象灾害风险数据库、山洪地质灾害指标库等资源的共享,已成为山洪灾害的及时监测、预警预报以及各类灾害预警信息传播的可靠稳定预警手段。     

高盐对肉鸡肺小动脉中膜平滑肌细胞表型转化的影响

观察了高盐诱发肉鸡腹水综合征（AS）发生发展过程中肺小动脉中膜平滑肌细胞（SMC）表型转化的规律，旨在探讨肺小动脉中膜SMC表型转化在AS发生过程中的可能作用。100只1日龄商品代AA雄性肉仔鸡常规育雏，8日龄起随机分为对照组和高盐组（饮水中添加0．30％氯化钠）。分别在15、22、29、36、43、50日龄时每组随机取5只鸡的肺组织并制备石蜡切片，以免疫组织化学方法结合图像分析法测定SMC表型标志蛋白α-SM-actin的表达。结果表明：（1）对照组肉鸡的生长发育过程中存在肺小动脉中膜SMC表型的动态转化，22日龄时发生了由收缩表型向增殖表型的转化，随后逐步向收缩表型回转，且各级肺小动脉SMC转化时间不一致。（2）高盐组SMC表型转化时间（15日龄）较对照组提前了1周，高盐促进了SMC由收缩表型向增殖表型转化。且直径20～50μm的肺小动脉SMC表型转化受高盐影响最大，推测其在高盐诱发肉鸡AS的过程中可能起更重要的作用。     

Effect of Dietary Lactobacillus fermentum from Pig on Growth Performance,Nutrient Apparent Digestibility,Faecal Microflora Number and Serum Immune Indices in Weaned Piglets

This experiment was conducted to study the effects of Lactobacillus fermentum on growth performance, nutrient apparent digestibility, faecal microflora number and serum immune indices of weaned piglets.A total of 160 weaned piglets with an average age of (30±2) days and averge body weight of (7.85±0.68) kg were randomly distributed into 4 groups with 4 replicates per group and 10 piglets per replicate feeding basal diet, 0.1%, 0.2%, and 0.3% Lactobacillus fermentum supplementation diets, respectively.The experiment lasted for 35 d.The results showed that, compared with the control group, the average daily gain, the feed conversion ratio, the apparent digestibility of crude protein, calcium and total phosphorusin of Lactobacillus fermentum groups were all increased in varying degrees.The optimum supplemental level of Lactobacillus fermentum should be 0.2%, and at this level, the average daily gain was significantly increased by 8.70% (P<0.05), the F/G was decreased by 4.62% (P<0.05), the apparent digestibility of crude protein, calcium and total phosphorus were significantly increased (P<0.05), the number of faecal E.coli was significantly decreased (P<0.05), and the serum IgG was significantly increased by 8.39% (P<0.05).The results indicated that Lactobacillus fermentum supplementation could improve the immune function, growth performance, the apparent digestibility of crude protein, calcium and total phosphorus and reduce the number of faecal E.coli of weaned piglets.     

不同层次的专科生学习《遗传学》的元认知意识研究

借助调查问卷,调查了不同层次的专科生在学习《遗传学》课程的元认知意识,揭示了两组学习者在主体知识、任务知识、策略知识、体验及控制能力等方面的差异,并提出培养其元认知能力的途径,以期为《遗传学》教学提供有益的指导。     

甜菜果胶-WPI复合对β-胡萝卜素乳液消化特性的影响

基于静电吸附原理和酶促交联原理,分别采用静电自组装与酶促交联技术对β-胡萝卜素乳液进行界面改性,将甜菜果胶层吸附于蛋白乳液界面。结果表明,以乳清分离蛋白(WPI)作为乳化剂,果胶质量分数为0.15%时,可以制备出两种改性β-胡萝卜素乳液。尽管改性乳液的粒径明显大于原乳液,但是,消化后β-胡萝卜素的生物有效率明显低于原乳液,表明改性后的β-胡萝卜素乳液具有一定的缓释功能。并且,果胶间以酶促交联形式存在的改性乳液受pH值的影响更小,缓释效果更突出。     

梨抗黑星病AFLP标记筛选

 Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears. Molecular markers linked to scab resistance gene is expected to be useful for improving pear. In this study, the F₁ population derived from the cross of ‘Huangguan’ and ‘Yali’ was analyzed genetically. The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner. Bulked segregant analysis (BSA) was conducted to screen 64 fluorescent AFLP primer pairs. A marker designated as D3-365 was found to be linked to the resistant locus. Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.     

生态旅游目的地空间结构发展演变研究——以河北省为例

在中外学者对旅游目的地空间结构研究的基础上,分析了生态旅游目的地的主要构成因素：生态旅游资源、旅游交通和旅游中心城市,并以河北省为例,介绍了其生态旅游发展存在的结构问题,确定了河北省的3级生态旅游系统：环京津1级生态旅游系统和以石家庄、邢台和邯郸为节点的2级生态旅游系统以及以衡水为节点城市的3级生态旅游系统。     

樟树林地土壤多环芳烃的含量及其分布特征

研究樟树林地和无林地土壤中PAHs的含量和分布特征,并对两种土地类型土壤中的PAHs含量水平进行评价.结果表明:两种土地类型土壤中PAHs主要积累在0～30 cm的表土层,并随土层加深而明显减少,各土层中以高环的PAHs组分为主,PAHs主要源于燃烧源;樟树林地土壤中PAHs含量明显低于无林地,无林地表土层中PAHs含量是樟树林地表土层的1.1倍;对两种土壤中的PAHs含量水平进行评价,得知樟树林地土壤PAHs超标5种,无林地土壤不仅超标8种,且超标倍数明显大于樟树林地,樟树林地土壤的PAHs污染程度小于无林地.