乌鸡、火鸡实验性组织滴虫病病理学比较

以组织滴虫病疫区鸡异刺线虫虫卵实验诱发火鸡、乌鸡组织滴虫病，分别在潜伏期、症状明显期、转归期对盲肠、肝脏等组织器官进行病理学比较观察，结果显示：盲肠卡他（乌鸡13/20；火鸡8/29）和纤维素性坏死（火鸡19/27，乌鸡7/20）。肝脏脂肪变性（火鸡8/27；乌鸡11/20）及局灶性坏死（火鸡19/27；乌鸡4/20）。此外，火鸡肝脏与盲肠见典型传染性肉芽肿。     

基于分子技术的柑橘黄龙病研究进展

黄龙病是柑橘生产上的一种毁灭性病害。近年来该病害在世界多个柑橘主产区发生与流行,使其再次引起了世界柑橘生产者和研究者的高度关注。目前认为柑橘黄龙病病原是一种韧皮部难养细菌,暂命名为"Candidatus Liberibacter spp."。随着现代分子生物学技术的迅猛发展,柑橘黄龙病相关菌的全基因组和柑橘全基因组序列日趋完善。我们在这一大背景下主要介绍近5 a来柑橘黄龙病分子水平的研究概况,特别是在分子流行学、基因组学、转录组学、蛋白质组学、病原致病性和寄主抗病性分子机理及防控方法等方面的进展,并对目前研究中存在的困难进行分析,对今后的研究方向进行展望。     

白菜叶和茎中微量元素Cu、Mn含量的测定

为研究白菜的食品应用及工业应用价值,采用HNO3-HCl O4(4∶1)湿法消解样品,火焰原子吸收光谱法(FAAS)测定白菜叶和茎中微量元素Cu、Mn的含量。试验结果表明,白菜叶和茎中Mn、Cu含量存在明显差异,Mn的含量均大于Cu的含量,且这两种元素在叶中的含量均高于茎。     

Effects of microglia-derived IL-1β on differentiation of OPCs in corpus callosum of septic neonatal rats

AIM: To explore whether IL-1β inhibits the oligodendrocyte precursor cell (OPCs) differentiation and affects axonal myelination. METHODS: One-day-old SD rats were randomly divided into control group and LPS group (48 rats in each group). The rats in LPS group were intraperitoneally injected with 1 mg/kg LPS. The rats in control group were injected with an equal volume of PBS. The rats in each group were further divided into 3 h, 24 h, 3 d, 7 d, 14 d and 28 d subgroups after injection. The expression of IL-1β and IL-1R₁ in the rat corpus callosum at 3 h, 24 h, 3 d, 7 d was determined by double immunofluorescence and Western blotting. The myelin basic protein(MBP) expression in the rat corpus callosum at 14 d, 28 d after injection was also measured. In vitro, primary OPCs culture was performed and divided into control group, 30 μg/L IL-1β group, 30 μg/L IL-1β+IL-1Ra group and 30 μg/L IL-1Ra group. The expression of MBP in the OPCs induced differentiation for 3 d was observed by double immunofluorescence and Western blotting. RESULTS: The expression of IL-1β and IL-1R₁ in the rat corpus callosum at 3 h, 24 h, 3 d, 7 d after LPS injection was obviously increased and the expression of MBP in the rat corpus callosum at 14 d, 28 d in LPS group was obviously decreased compared with control group in vivo. The level of MBP was significantly decreased after IL-1β treatment for 3 d in vitro. However, IL-1Ra (IL-1R inhibitor) reversed the down-regulation of MBP expression. IL-1β inhibited the expression of p-ERK, ERK over-expression reversed the down-regulation of MBP expression compared with IL-1β group. CONCLUSION: IL-1β inhibits the differentiation of OPCs, which may be involved in ERK pathways, thus leading to axonal hypomyelination in the corpus callosum of septic neonatal rats.     

温室黄瓜新品种津优335号的选育

津优335号是以自交系A143-5为母本,以自交系B74A-7为父本配制而成的黄瓜一代杂种。耐低温弱光能力强,植株长势强,雌花节率高、连续结瓜能力强,瓜条生长速度快。瓜条商品性突出,品质好。抗枯萎病,中抗褐斑病、霜霉病和白粉病。越冬栽培不歇秧,单瓜质量约220g,总产量20000kg·(667m2)-1左右,适宜我国北方地区日光温室各茬口栽培。     

辣椒新品种海丰1052

海丰1052是以08-123为母本,F07-253-2-1为父本配制的早熟鲜食线椒一代杂种。始花节位为第7~8节,果实顺直,果面光滑,果肩略皱,辣味较强,青熟果亮绿色,老熟果鲜红色,果实纵径31.2cm,横径1.5cm,平均单果质量为32.6g,VC含量为672mg·kg-1,可溶性糖含量1.7g·kg-1,蛋白质含量为14.0g·kg-1,辣椒素含量为0.25g·kg-1。抗CMV、TMV,中抗疫病。一般每667m2产量3000kg左右。适合北方露地、秋延后大棚栽培,广东、广西、山西、陕西等地露地栽培。     

Cx43 is involved in electrical remodeling of atrial myocytes through regulating L-type calcium current

AIM: To investigate whether the association of connexin 43(Cx43) and L-type calcium channel involved in the pathogenesis of atrial fibrillation (AF). METHODS: The biochemical assays and whole-cell patch-clamp technique were used to study the expression of Cx43 in human atrial tissue. The co-localization of Cx43 and L -type calcium channel, and the regulation of L-type calcium current in atrial myocytes were investigated. RESULTS: The expression of Cx43 at mRNA and protein levels was decreased in human atrial tissues of AF patients. In cultured atrium-derived myocytes (HL-1 cells), knockdown of Cx43 significantly inhibited the mRNA expression of L-type calcium channel α1c subunit, as well as L-type calcium current. Co-localization of Cx43 with L-type calcium channel α1c subunit in mouse atrial myocytes was observed. CONCLUSION: The decrease in Cx43 is involved in the pathogenesis of AF, probably through reducing the L-type calcium current in atrial myoctyes by co-localization with L-type calcium channel, thus representing the potential pathogenesis in atrial fibrillation.     

Expression of Jagged2/Notch3 signaling molecules in pulmonary hypertensive rats induced by monocrotaline

AIM: To study the expression of Jagged2/Notch3 signaling molecules in pulmonary vascular wall of pulmonary hypertensive rats induced by monocrotaline. METHODS: SD rats were randomly divided into normal control group (C group,n=15), solvent control group (S group,n=15) and monocrotaline model groups (M group,n=15). The model of pulmonary hypertension was established by a single subcutaneous injection of monocrotaline (50 mg/kg). The rats in S group were given a single subcutaneous injection of the same dose of solvent. After 4 weeks, the pulmonary vascular remodeling was assessed by HE staining, and the mean pulmonary artery pressure (mPAP) and right ventricular systolic pressure (RVSP) were determined by right heart catheterization. The expression of Jagged2/Notch3/Hes5 molecules in the pulmonary vascular wall was detected by immunohistochemical method and real-time PCR. RESULTS: Compared with S group and C group, the percentage of medial wall thickness of smaller arteries in model group increased significantly (P<0.01). The levels of mPAP and RVSP in M group were significantly higher than those in S group and C groups (P<0.01). The results of real-time PCR showed that the expression of Jagged2, Notch3 and Hes5 was significantly increased in M group compared with S group and C group. The data from immunohistochemical detection indicated that Jagged2 mainly expressed in the intima of small lung artery, Notch3 and Hes5 mainly expressed in the medial smooth muscle cells. Compared with S group and C group, the expression of Jagged2 and Notch3 was significantly increased in the lung small arteries of M group. CONCLUSION: The activation of Jagged2/Notch3 signaling pathway might play an important role in the formation of pulmonary hypertension.     

华灰莉木提取物对柑橘全爪螨的杀螨活性

以华灰莉木为试材,采用溶剂冷浸法提取植物成分,溶剂液-液萃取法对植物成分进行初步分离,Potter喷雾法测定杀螨活性,研究华灰莉木提取物和初步分离物对柑橘全爪螨的影响。结果表明:华灰莉木叶和枝条甲醇提取物对柑橘全爪螨雌成螨有较高的触杀毒力,致死中浓度(LC50)分别为5.5334g/L和3.9682g/L。叶的杀螨活性物质主要集中在水萃取物中,属于极性强的物质;枝条的杀螨活性物质主要集中在石油醚萃取物中,属于极性弱的物质。叶提取物的水萃取物和枝条提取物的石油醚萃取物对柑橘全爪螨雌成螨的LC50分别为3.2970g/L和1.7372g/L。     

水稻籼爪交和粳爪交杂种优势的研究Ⅰ.杂种农艺性状的表现

研究了籼爪交和粳爪交杂种在长沙、三亚两地农艺性状和干物质产量的表现. 由引进爪哇稻品种所配的籼/爪和粳/爪交杂种F1代表现为植株高大, 生长茂盛, 具有强大的干物质产量优势. 绝对干物质产量(单株穗期干重)为: 籼爪杂种＞粳爪杂种＞对照(籼籼交杂种), 干物质生产率(单株日干物质产量)为: 籼爪杂种＞粳爪杂种＞对照. 供试籼