全文获取类型
收费全文 | 31266篇 |
免费 | 1796篇 |
国内免费 | 3465篇 |
专业分类
林业 | 2715篇 |
农学 | 2731篇 |
基础科学 | 1895篇 |
3606篇 | |
综合类 | 13688篇 |
农作物 | 2371篇 |
水产渔业 | 1736篇 |
畜牧兽医 | 4406篇 |
园艺 | 1935篇 |
植物保护 | 1444篇 |
出版年
2024年 | 207篇 |
2023年 | 524篇 |
2022年 | 1192篇 |
2021年 | 1272篇 |
2020年 | 1239篇 |
2019年 | 1178篇 |
2018年 | 851篇 |
2017年 | 1409篇 |
2016年 | 1125篇 |
2015年 | 1497篇 |
2014年 | 1631篇 |
2013年 | 1906篇 |
2012年 | 2542篇 |
2011年 | 2617篇 |
2010年 | 2454篇 |
2009年 | 2192篇 |
2008年 | 2123篇 |
2007年 | 2026篇 |
2006年 | 1742篇 |
2005年 | 1342篇 |
2004年 | 913篇 |
2003年 | 794篇 |
2002年 | 993篇 |
2001年 | 850篇 |
2000年 | 689篇 |
1999年 | 314篇 |
1998年 | 169篇 |
1997年 | 150篇 |
1996年 | 76篇 |
1995年 | 99篇 |
1994年 | 102篇 |
1993年 | 66篇 |
1992年 | 55篇 |
1991年 | 54篇 |
1990年 | 31篇 |
1989年 | 24篇 |
1988年 | 25篇 |
1987年 | 18篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1981年 | 8篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1962年 | 4篇 |
1956年 | 3篇 |
1932年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 9 毫秒
31.
为提供番茄灰霉病的早期诊断技术,应用其病原菌灰葡萄孢种特异片段P729分子标志,建立了PCR检测方法,并进行了实验室和大田现场标本的评价。结果显示番茄灰霉病的分子诊断方法在实验室具有特异、稳定和可靠的特点。比较6种抽提基因组DNA的方法,方法B效果理想。应用建立的分子诊断方法可从0.2μg灰葡萄孢菌丝中获得阳性扩增,具有相当高的灵敏性;大田样本的检测结果说明该方法可用于番茄灰霉病的早期诊断。 相似文献
32.
33.
34.
挤压是一种常用的食品和饲料加工方法,挤压过程中的高温、高压和机械剪切的综合作用对加工中各种配料的营养成分会产生重要影响,并且因此而影响到饲喂动物的生长性能。通过对小麦、大麦、豆粕和玉米蛋白等常用配料在挤压前后化学成分变化情况的对比分析,指出了挤压加工的配料其生物有效性、饲料转化率、表观消化率及微量元素等指标变化对鱼类生长性能的重要影响。 相似文献
35.
36.
卵泡颗粒细胞维持山羊卵母细胞减数分裂抑制状态的研究 总被引:1,自引:0,他引:1
通过在卵母细胞体外成熟过程中添加不同浓度的卵丘细胞或其外围壁层颗粒细胞进行共培养,对山羊卵母细胞成熟过程中卵泡颗粒细胞对卵母细胞减数分裂抑制作用及作用机制进行了探索.结果表明,成熟液中添加105和106个/mL卵丘细胞或外围壁层颗粒细胞的卵母细胞体外成熟率与对照组相比差异不显著(P>0.05),但添加5×106个/mL(41.2%)和107个/mL(24.6%)卵丘细胞或外围壁层颗粒细胞组的成熟率显著降低(P<0.05);添加5×106和107个/mL卵丘细胞组和颗粒细胞组卵丘不能正常扩展,卵母细胞核状态观察,停留在GV期;Rp-cAMP(Rp-cyclic adenosine monophosphothioate)膜渗透性cAMP对抗剂,诱导了107个/mL的卵丘细胞组(71.6%)和外围壁层颗粒细胞组(71.3%)的卵母细胞体外成熟率与对照组(72.O%)差异不显著(P>0.05).在山羊卵母细胞体外成熟过程中,添加一定浓度的卵丘细胞及其外层的颗粒细胞可以提高卵母细胞胞质内的cAMP的水平,抑制卵母细胞减数分裂的启动,使卵母细胞停留在GV期. 相似文献
37.
今年是我国家具出口企业第三个困难年,大企业利润下降,中小企业效益滑坡。大面积亏损,主要原因是原材料价格继续上涨,美元贬值和人民币升值三大因素。 相似文献
38.
Sayed Fathi El-Hawari Norihiko Oyama Yukako Koyama Jun Tamura Takaharu Itami Tadashi Sano Kazuto Yamashita 《Journal of veterinary science (Suw?n-si, Korea)》2022,23(4)
BackgroundProblems associated with using inhalational anaesthesia are numerous in veterinary anaesthesia practice. Decreasing the amount of used inhalational anaesthetic agents and minimising of cardiorespiratory disorders are the standard goals of anaesthetists.ObjectiveThis experimental study was carried out to investigate the sparing effect of intravenous tramadol, lidocaine, dexmedetomidine and their combinations on the minimum alveolar concentration (MAC) of sevoflurane in healthy Beagle dogs.MethodsThis study was conducted on six beagle dogs. Sevoflurane MAC was determined by the tail clamp method on five separate occasions. The dogs received no treatment (control; CONT), tramadol (TRM: 1.5 mg kg-1 intravenously followed by 1.3 mg kg-1 h-1), lidocaine (LID: 2 mg kg-1 intravenously followed by 3 mg kg-1 h-1), dexmedetomidine (DEX: 2 μg kg-1 intravenously followed by 2 μg kg-1 h-1), and their combination (COMB), respectively. Cardiorespiratory variables were recorded every five minutes and immediately before the application of a noxious stimulus.ResultsThe COMB treatment had the greatest sevoflurane MAC-sparing effect (67.4 ± 13.9%) compared with the other treatments (5.1 ± 25.3, 12.7 ± 14.3, and 40.3 ± 15.1% for TRM, LID, and DEX treatment, respectively). The cardiopulmonary variables remained within the clinically acceptable range following COMB treatment, although the mean arterial pressure was higher and accompanied by bradycardia.ConclusionsTramadol-lidocaine-dexmedetomidine co-infusion produced a remarkable sevoflurane MAC-sparing effect in clinically healthy beagle dogs and could result in the alleviation of cardiorespiratory depression caused by sevoflurane. Cardiorespiratory variables should be monitored carefully to avoid undesirable side effects induced by dexmedetomidine. 相似文献
39.
40.
Ken R. Ito Tomonori Sato Hiro Goto Katsuyoshi Sato Jun Watanabe Masaki Yokoo 《The Journal of Poultry Science》2022,59(3):247
Increasing food loss and waste (FLW) is a global problem, and efforts are being made to use waste food as potential livestock feed material. The amount of self-supplied feed is lower in Japan than in other countries, and the government recommends FLW use for animal feed. Sake (Japanese rice wine) is a traditional alcoholic beverage. During the sake manufacturing process, large amounts of squeezed solids or “lees” (sake lees) are generated. Sake lees are nutritious and functional, but are prone to spoilage. In this study, we investigated whether sake lees should be mixed with animal feed immediately or after drying. To assess the usefulness of sake lees as a poultry feed ingredient and determine the effect of sake lees on intestinal immunity, we performed a feeding trial with three treatments: a raw sake lees (RSL) diet, dried sake lees (DSL) diet, and control diet. Three-week-old broilers were fed these diets (n=8 per group) for two weeks. We then calculated feed efficiency and performed RT-qPCR to assess the effects of diet on intestinal immunity. The growth performance in the RSL diet group was equivalent to that in the control diet group. The DSL diet became difficult for broilers to eat, resulting in decreased growth performance. In the ileum of RSL-diet broilers, the mRNA expression levels of TGF-β1 and avian β-defensin (AvBD)12 were significantly increased compared to those of control diet broilers (p<0.05), and a significant correlation was observed between the two genes (p<0.05). Our results indicated that sake lees should not be dried and should be mixed immediately with feed, and this sake lees when fed to chicken activates the intestinal immunity. However, sake lees have a lower fat content than corn, and it is thus important to combine sake lees with high-energy feed. 相似文献