全文获取类型
收费全文 | 77篇 |
免费 | 5篇 |
专业分类
林业 | 5篇 |
农学 | 22篇 |
基础科学 | 2篇 |
10篇 | |
综合类 | 4篇 |
农作物 | 8篇 |
水产渔业 | 1篇 |
畜牧兽医 | 19篇 |
园艺 | 6篇 |
植物保护 | 5篇 |
出版年
2022年 | 1篇 |
2020年 | 2篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 5篇 |
2015年 | 3篇 |
2014年 | 6篇 |
2013年 | 1篇 |
2012年 | 4篇 |
2011年 | 2篇 |
2010年 | 2篇 |
2009年 | 5篇 |
2008年 | 5篇 |
2007年 | 10篇 |
2006年 | 3篇 |
2005年 | 5篇 |
2004年 | 4篇 |
2003年 | 5篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有82条查询结果,搜索用时 468 毫秒
21.
Alejandro A. Royo Susan L. Stout David S. deCalesta Timothy G. Pierson 《Biological conservation》2010,143(11):2425-2434
White-tailed deer (Odocoileus virginianus) overbrowsing has altered plant species diversity throughout deciduous forest understories in eastern North America. Here we report on a landscape-level (306 km2) project in Pennsylvania, USA that tracked the herbaceous community response to deer herd reductions. From 2001 to 2007, we estimated deer densities, browse impact on woody seedlings, and censused the herbaceous flora in permanent plots throughout the area. We assessed herb layer species richness, abundance, and dominance and measured three known phytoindicators of deer impact: Trillium spp., Maianthemum canadense, and Medeola virginiana. We predicted that browse-sensitive taxa would increase in abundance, size, and flowering as would overall species diversity following deer culls and browse impact that declined by an order of magnitude by 2007. Following intensified deer harvests, we observed a limited recovery of the herbaceous community. Trillium spp. abundance, height, and flowering; M. canadense cover; and M. virginiana height all increased following herd reductions. Similarly, forb and shrub cover increased by 130% and 300%, respectively. Nevertheless, species diversity (i.e., richness and dominance) did not vary. Our work demonstrates that reducing deer densities can provide rapid morphological and population-level benefits to palatable species without a concomitant recovery in diversity. We suggest that decreasing deer populations alone may not promote plant diversity in overbrowsed, depauperate forests without additional restoration strategies to mitigate a browse-legacy layer dominated by browse-resistant species. 相似文献
22.
Alvarez I Gutiérrez JP Royo LJ Fernández I Gómez E Arranz JJ Goyache F 《Journal of animal science》2005,83(4):737-744
Recent studies have proposed the use of molecular coancestry coefficients as a measure of genetic variability and as a useful tool for conservation purposes. Using simulated data, molecular coancestry has been shown to become constant very quickly after separation of populations, leading to population diversity remaining constant. However, the use of molecular coancestry information to study the genetic relationships between breeds has not yet been widely explored. Here we analyze the polymorphism of 14 microsatellites in 222 unrelated individuals belonging to seven native Spanish breeds to ascertain the usefulness of molecular coancestry-based methodologies in providing information on their genetic relationships. Average kinship distance (D(k)) and average molecular coancestry coefficients (f(ij)) were compared with well-known genetic distances, such as between-breed Reynolds' distance (D(R)), Nei's standard distance (D(s)), and shared allele distance (D(AS)). Kinship distance and f(ij) have moderate to low correlations with the other genetic distances, showing that they provide different information: both D(k) and f(ij) account for the allele frequencies in the founder population, whereas D(R), D(s), and D(AS) characterize the short-term evolution of the populations. Furthermore, D(k) and f(ij) were only moderately correlated (-0.500). The present study used field data to confirm previous research pointing out the ability of molecular coancestry coefficients to assess genetic differentiation of an ancestral origin. In this respect, molecular coancestry-based parameters may be used with classical genetic parameters to obtain information on population dynamics in livestock breeds. This study additionally presents reliable evidence on the history of these sheep breeds. 相似文献
23.
Alvarez I Royo LJ Fernández I Gutiérrez JP Gómez E Goyache F 《Journal of animal science》2004,82(8):2246-2252
Although many research papers have studied diversity and differentiation within livestock species, genetic relationships among neighboring populations remain poorly understood. Here we apply recent methodologies to analyze the polymorphism of 14 microsatellites in 238 unrelated individuals belonging to six sheep breeds from Northern Spain to ascertain their historical relationships and the relative genetic contributions existing between populations. Individual genotypes were analyzed to assess the existence of an underlying genetic structure. Long-term and recent migration rates were estimated to identify patterns of relative genetic contribution among breeds. The complete data set showed a strong population structure derived from both different ancestral origins and some geographical patterns of recent gene flow. Two of the analyzed breeds (Black-faced Latxa and Churra) had a marked genetic background, supporting the hypothesis that, regardless of their phenotypical similarities, they have different ancestral origins. Some of the more presumably related breeds had negative long-term admixture coefficients, showing that they diverged only recently. In addition, we show how methodologies for estimation of long-term gene flow and recent patterns of migration are complementary, providing information about migration rates on different timescales. 相似文献
24.
Berry weight (BW) and sugar concentration (SC) are relevant indices in viticulture since they can be easily measured and, when considered together, give a relatively fair estimation of grape quality. This work aims to evaluate the influence of water availability, leaf area and fruit load on BW and SC, estimating the relative importance of these factors. Leaf area (LA), berry number (BN), yield (YLD), water potential in summer (ψpd-s), BW and SC were measured in 16 and 17 ‘Tempranillo’ vineyards in 1999 and 2000, respectively. In all the vineyards, according to local practices, the irrigation amount decreased as summer progressed. The study vineyards comprised a broad range of situations concerning leaf area, fruit load and water status in summer. Average leaf water potential in summer and LA/BN ratio, when considered together, estimated properly BW (R2 = 0.91; P < 0.001) and, in a similar way, ψpd-s and LA/YLD ratio estimated SC (R2 = 0.74; P < 0.001). The relative weight of ψpd-s in both relationships was much higher than that of any of the LA:fruit ratios, showing that, under semiarid conditions, water availability plays the main role in regulation of berry growth and sugar accumulation and, therefore, the highest attention should be paid in these areas to irrigation management, seeking the degree of stress that allows optimizing the combination of yield and berry quality in each situation. 相似文献
25.
Javier Diéguez‐Uribeondo Félix Royo Catherine Souty‐Grosset Anne Ropiquet Frédéric Grandjean 《水产资源保护:海洋与淡水生态系统》2008,18(1):19-31
- 1. A study on the genetic variability of the white‐clawed crayfish was carried out based on mitochondrial cytochrome oxidase subunit I gene sequences. The sequences applied were more informative regarding white‐clawed crayfish genetic variability than others previously used.
- 2. Two haplotypes were found to exist in the Iberian Peninsula. The haplotypes exhibit a strong geographic subdivision (ΦST=0.83). One of the Iberian haplotype s was similar to north Italian haplotypes and the second differed in only one mutation. This pattern of genetic variability contrasts with those found in glacial refugial areas of France, Italy and the Balkan Peninsula.
- 3. Two hypotheses on the origin of the white‐clawed crayfish in the Iberian Peninsula are discussed: (i) one based on an anthropogenic origin, and (ii) a second based on a successive number of postglacial ancient and recent bottlenecks, i.e. the disjunction between Iberian and Italian populations of white‐clawed crayfish species is due to competition between A. italicus and A. pallipes, in addition to the impact of crayfish plague and human translocations.
- 4. New references for the white‐clawed crayfish in the Iberian Peninsula were found in medieval and Arabic texts. The results show that this species has been thriving in this peninsula since ancient periods and that its indigenous status should not be questioned.
- 5. Conservation action and plans should consider the low genetic diversity as a limitation for farm‐raising specimens more adapted and resistant to changing environments and diseases.
26.
Genetic diversity loss due to selection for scrapie resistance in the rare Spanish Xalda sheep breed
I. lvarez L.J. Royo J.P. Gutirrez I. Fernndez J.J. Arranz F. Goyache 《Livestock Science》2007,111(3):204-212
The effect of selection for scrapie resistance on genetic variability in the rare Xalda sheep breed was studied. Pedigree information comprised 1851 animals (1444 alive) at the moment of sampling. A total of 304 reproductive (or selected for reproduction) Xalda individuals were sampled and genotyped for 14 microsatellites. Genetic variability was assessed using: gene diversity (1 − average kinship, GD), mean average relatedness (AR) and self-coancestry (ci) at the genealogical level; and expected heterozygosity (He), molecular mean kinship (Mk), molecular self-coancestry (si) and rarefacted average number of alleles per locus (A) at the molecular level. Two breeding strategies were evaluated: a) use of only young rams with genotype ARR/ARR and young ewes with low to moderate risk (risk groups R1 to R3); b) breeding without selection for PrP genotypes. The major cause of losses of genetic variability in the Xalda breed is the drift that occurs when a new group of reproductive individuals is selected. The loss of genetic variability is small in females compared to the males, where it is considerable. However, losses at the molecular level for young females with respect to adult females were above 5%. Young male individuals also retained most of the genetic variability assessed in adult rams. Selection against susceptibility to scrapie produced additional losses of more than 2% for He and A when rYF individuals are considered. As regards males, the situation becomes critical because of the scant number of available ARR/ARR young rams. The consequences for the management of the Xalda breed are discussed. 相似文献
27.
Royo F Andersson G Bangyeekhun E Múzquiz JL Söderhäll K Cerenius L 《Veterinary microbiology》2004,104(1-2):103-112
Five Aphanomyces strains were isolated during suspected outbreaks of crayfish disease in Spain and Italy. Genetic and physiological evidence show that the strains isolated from the freshwater crayfish Procambarus clarkii and Pacifastacus leniusculus, do not fit into any previously identified group of Aphanomyces astaci and are not capable of killing crayfish following standardised experimental infection. RAPD-PCR and ITS sequencing analysis show a high degree of similarity between the new isolates, while they are clearly different from the A. astaci reference strains. They do, however, possess some properties, which are commonly associated with parasitic species such as repeated zoospore emergence and the lack of sexual reproduction. The five isolates share some physiological properties i.e. a high growth rate, and germination in response to nutrients and, in contrast to A. astaci, they do not express chitinase constitutively during growth or sporulation. Until their taxonomic status is fully elucidated we suggest that the new isolates be given the tentative species name Aphanomyces repetans. 相似文献
28.
Marc Moragues Jorge Zarco-Hernández Ma Angeles Moralejo Conxita Royo 《Genetic Resources and Crop Evolution》2006,53(5):993-1002
The genetic diversity of high and low molecular weight glutenin subunits of 63 durum wheat landraces from different geographical
regions in the Mediterranean Basin was studied using SDS-PAGE. Great variability in glutenin composition was found, with 42
high and low molecular weight glutenin haplotypes, 20 allele combinations at the HMW-GS loci, and 18 at the LMW-GS. All five possible LMW models were detected in all Mediterranean regions. Rare alleles were found at Glu-B1 locus in high frequencies and a priori related alleles to grain quality were also observed. Global genetic diversity index was relatively high (0.67); it ranged
from 0.33 to 0.66. Cluster analysis on the frequency patterns of origins grouped genotypes following a geographical structure.
Rogers’ distance coefficient on frequency pattern for each region of origin showed two germplasm pools with distinct quality
profiles, where South West Asian landraces were very different from the landraces of other Mediterranean areas. The relationship
between different regions of origin is discussed and two possible ways of introduction of wheat in the Iberian Peninsula (N
Africa and SE Europe) are hypothesized. The use of Mediterranean durum wheat landraces as source of genetic variability for
grain quality improvement is highly recommended. 相似文献
29.
Environmental and genetic determination of protein content and grain yield in durum wheat under Mediterranean conditions 总被引:1,自引:0,他引:1
Y. Rharrabti D. Villegas L. F. Garcia del Moral N. Aparicio S. Elhani C. Royo 《Plant Breeding》2001,120(5):381-388
The unpredictability of the Mediterranean climate causes fluctuations in wheat yield and quality, but offers the opportunity for obtaining high‐quality durum wheat in terms of grain protein content. Twenty‐five durum wheat genotypes were grown under irrigated and rainfed conditions at each of two latitudes in Spain during 1998 and 1999. Differences between latitudes in grain protein content and chlorophyll content in the flag leaf were attributable to nitrogen fertilization management. Cycle length until anthesis was less affected by the environment than grain‐filling duration, and was longer under irrigated conditions than in the rainfed sites. A negative asymptotic curve was the best equation to fit the relationship between yield and protein content, suggesting that yield improvements in fertile environments may be attained with negligible reductions in protein content. ‘Jabato’, ‘Waha’, ‘Lagost‐3’, ‘Massara‐1’ and ‘Vit?on’ showed medium to high yield, yield stability and high protein content. Chlorophyll content in the flag leaf, measured at anthesis with the soil‐plant analysis development (SPAD) portable field unit, may be useful for the fast and cheap detection of durum wheat genotypes with high grain protein content in drought‐stressed Mediterranean environments. 相似文献
30.
Many bioactive products from benthic invertebrates mediating ecological interactions have proved to reduce predation, but their mechanisms of action, and their molecular identities, are usually unknown. It was suggested, yet scarcely investigated, that nutritional quality interferes with defensive metabolites. This means that antifeedants would be less effective when combined with energetically rich prey, and that higher amounts of defensive compounds would be needed for predator avoidance. We evaluated the effects of five types of repellents obtained from Antarctic invertebrates, in combination with diets of different energetic values. The compounds came from soft corals, ascidians and hexactinellid sponges; they included wax esters, alkaloids, a meroterpenoid, a steroid, and the recently described organic acid, glassponsine. Feeding repellency was tested through preference assays by preparing diets (alginate pearls) combining different energetic content and inorganic material. Experimental diets contained various concentrations of each repellent product, and were offered along with control compound-free pearls, to the Antarctic omnivore amphipod Cheirimedon femoratus. Meridianin alkaloids were the most active repellents, and wax esters were the least active when combined with foods of distinct energetic content. Our data show that levels of repellency vary for each compound, and that they perform differently when mixed with distinct assay foods. The natural products that interacted the most with energetic content were those occurring in nature at higher concentrations. The bioactivity of the remaining metabolites tested was found to depend on a threshold concentration, enough to elicit feeding repellence, independently from nutritional quality. 相似文献