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131.
Youwen You Cornelius E. Uboh Lawrence R. Soma Fuyu Guan Daniel Taylor Xiaoqing Li Ying Liu Deborah Tsang 《Journal of Equine Veterinary Science》2014
A liquid chromatography–tandem mass spectrometry method was developed to confirm the presence of androstenedione-19-oic acid in intact male equine plasma and to show the source of 19-norandrostenedione in equine plasma. Androstenedione-19-oic acid was recovered from acidified plasma by liquid–liquid extraction using methyl tert-butyl ether and separated on an Ace 5 C8 column. A triple quadrupole mass spectrometer was used to detect the analytes in negative electrospray ionization mode. Limits of detection, quantification, and confirmation of the method were 0.1, 0.5, and 1.0 ng/mL, respectively. The linear dynamic range of quantification was 0.5–50 ng/mL. The presence of androstenedione-19-oic acid was confirmed in all plasma samples obtained from intact male horses but not those from gelded and female horses; the average concentration was 3.1 ± 1.6 ng/mL, suggesting androstenedione-19-oic acid is an endogenous compound only in intact male horse plasma samples. The conversion of androstenedione-19-oic acid to 19-norandrostenedione in equine plasma was demonstrated by spiking androstenedione-19-oic acid into blank plasma and monitoring the generation of 19-norandrostenedione and its increase in concentration during storage. Results indicated that androstenedione-19-oic acid was readily converted into 19-norandrostenedione; the higher the storage temperature, the faster the conversion. The conversion was not affected by the types of plasma samples collected from gelded and female horses or by anticoagulants used in blood collection to harvest plasma. Compared with other matrices such as water, methanol, and phosphate-buffered saline, the conversion of androstenedione-19-oic to 19-norandrostenedione in equine plasma was faster, suggesting that there is an unknown factor(s) in equine plasma that enhances the conversion. 相似文献
132.
珠江卷口鱼年龄生长、食性和繁殖的研究 总被引:5,自引:2,他引:5
本文报道了珠江卷口鱼的年龄生长、食住和繁殖方面的研究结果。通过分析确定:卷口鱼的年龄级由I—IV龄鱼组成,各龄鱼分别占渔获物总量的42.21%、38.21%、19.21%和0.38%。用统计学方法,计算出卷口鱼体长与鳞长的相关式为:L=8 43.30R(r=0.9956);体长与体重的相关式为:W=8.226×10~(-3)L~(3.374)(Γ=0.9654)。对卷口鱼各种食物的组成、季节变化及出现率作了分析探讨。卷口鱼个体绝对怀卵量波动于7878—48880粒之间,平均22250.7粒。绝对怀卵量与体长和体重的相关式为:R=4.5419L~(3.6208)和R=40.88W 1209. 文中对卷口鱼的资源繁殖保护,网具使用和合理捕捞规格等问题进行了讨论。 相似文献
133.
虾池沉积物中3类主要细菌的垂直分布特征 总被引:1,自引:0,他引:1
采用平板涂布法和MPN法测定了虾池底质下 0到 30cm深度范围内 3类主要细菌类群的垂直分布情况。结果表明 ,底泥中细菌主要集中于 0到 5cm的表层范围内 ,随深度增加 ,数量急剧减少 ,至 30cm深处所测到菌量已很少。底泥中的总菌量随养殖时间推移 ,逐渐增加 ,到养殖中后期 ,表层菌量增加至 10 6CFU/g ,表层以下 10~2 0cm的总异养菌量和硝酸盐还原菌数量也增加至 10 5CFU/ g以上。弧菌仍集中于表层。细菌的垂直分布主要受各层有机物和溶解氧含量的影响 相似文献
134.
135.
Mixing is a mechanical operation to distribute components evenly in a plane or a space. Composite molding and textile manufacturing industries are routinely taking advantage of mixing operations in order to distribute raw materials uniformly or in a specific way during composite molding or textile producing processes. Key issues to identify any mixed state are (1) the time needed to accomplish through mixing (the time scale) and (2) the extent of the homogeneity of the mixture in a plane (the length scale). In this paper, a new concept of mixture status is focused on the length scale. The goal to derive a new concept of mixture status is to establish a measure to describe the mixed extent of a mixture consisting of two components, derived from clump size and clump distribution. To verify the usefulness of the newly conceived “Mixedness Index”, the suggested definition was compared with the existing coefficient of variation (CV) method based on simulation and experiments. Results showed a reasonable relationship between the Mixedness Index and observation, demonstrating the feasibility of the proposed Mixedness Index for industrial applications. 相似文献
136.
You Xu Guibin Wang Fuliang Cao Cancan Zhu Guangyu Wang Yousry A. El-Kassaby 《New Forests》2014,45(6):765-776
Response of growth and secondary metabolites to light intensity are useful measurements to determine suitable silviculture treatments for the cultivation of medicinal plants. Here, we analyzed the growth, flavonols (total flavonol, quercetin, kaempferol, and isorhamnetin) content, flavonols yield per plant, and expression of flavonoid biosynthesis-related genes in 2-year Ginkgo (Ginkgo biloba L.) seedlings at four different light intensities (100, 76, 40, and 25 % of full sunlight) in a greenhouse setting. Across all light intensities, the 76 % sunlight treatment produced the highest growth of total biomass, root, stem, and leaf, indicating negative effects of either fulllight or heavy shading on Ginkgo seedling development. Both flavonols (total flavonol, quercetin, kaempferol, and isorhamnetin) content and expression of flavonoid biosynthesis-related genes [PAL (Phenylalanine ammonia-lyase), CHS (Chalcone synthase), F3H (Flavanone 3-hydroxylase), and FLS (Flavonol synthase)] in leaves were highest under 100 % sunlight, suggesting that full sunlight promotes the expression of flavonoid biosynthesis-related genes and increases flavonoid biosynthesis. The highest and lowest flavonol contents were found in leaves and stems, respectively. The 76 % sunlight treatment produced the highest flavonols yield while the 100 % sunlight produced the highest flavonoids content in leaves, indicating that flavonol production per unit land area depends not only flavonol content but also biomass. Overall, in order to achieve the highest flavonols yield per area in Ginkgo leaf-harvesting plantations, it is important to manipulate light conditions of field. 相似文献
137.
138.
以果蔗(Badila)茎尖脱毒组培苗为材料,利用间歇浸没式生物反应器(Temporary immersion bioreactors TIBs)进行组培快繁的技术体系的研究。结果表明:(1)使用TIBs系统进行甘蔗组织培养一代增殖较传统方法高出10倍以上;(2)TIBs系统以第4代继代材料为宜;(3)接种密度在10~15株/L培养基最有利于甘蔗组培苗的增殖和生长;(4)TIBs系统中6-BA浓度为0.5~1.0mg/L时适合于增殖培养,NAA浓度为4mg/L时有利于根的诱导;(5)浸没间歇频率在1min/3h时增殖和生长表现较为优异,1min/6h时有利于根的诱导和根的生长。 相似文献
139.
140.
Xia Li Sichao Wang Zhangjun Jiang Xinglong Li Birong You Chengjie Rui 《Soil Use and Management》2023,39(1):298-315
This paper reports research based on pneumatic subsoiling and the design of a pneumatic subsoiling mechanism to overcome the problems of high resistance and high energy consumption of subsoiling. By analysing soil-specific resistance, soil disturbance rate and soil bulkiness under different air pressure conditions, it is concluded that pneumatic subsoiling can effectively break the soil plough pan and reduce resistance to subsoiling. In order to analyse the impact of air pressure on subsoiling, in this study, principal component analysis was used to analyse the pneumatic subsoiling disturbance parameters (working air pressure, working depth and working speed), and the test results show that the contribution of air pressure to subsoiling resistance and subsoiling disturbance surface reached 24% and 25%, respectively. An orthogonal test was used to analyse the specific resistance of subsoiling, and its significance coefficient is 0.95. Long short-term memory neural networks (LSTM) and bidirectional long short-term memory neural networks Bi-LSTM. are used to predict the cracks on the disturbed surface of subsoiling. LSTM is a method to predict future occurrence using time series data, which can be used to predict the cracks on the disturbed surface of soil, while Bi-LSTM network is an innovative computing paradigm, which learns bidirectional long-term correlation between time step and sequence data, to predict the trend of fissures on the disturbed soil surface. The RSME of LSTM and Bi-LSTM are 4.80 and 6.55, and their determinative factor R2 is 0.95 and 0.94 respectively, which indicates that LSTM and Bi-LSTM can effectively predict the cracks of pneumatic subsoiling. By analysing the specific resistance of pneumatic subsoiling, it can be shown that pneumatic subsoiling can reduce subsoiling resistance and expand the disturbance surface of subsoiling so as to achieve the effects of subsoiling, drag reduction and reduction of fuel consumption. 相似文献