Isoflavone profile and biological activity of soy bread

The present study examines the ability of isoflavone extracts from whole soy bread and two soy bread fractions, crumb and crust, to modulate the proliferation of human prostate cancer PC-3 cells. Total isoflavone content in the two fractions of soy bread were similar (3.17 micromol/g dry basis). However, their conjugate patterns were altered. Both fractions of soy bread contained a similar level of isoflavone aglycones ( approximately 24%). Low concentrations of soy bread extracts increased PC-3 cell proliferation as much as 47% compared to untreated control. This proliferative effect in cell growth was reduced at higher extract concentration. Soy bread crust extract (10 mg/mL) reduced PC-3 cell proliferation by 15% compared to untreated control. Interestingly, wheat bread extracts increased cell proliferation at all concentrations tested. Although extracts from both breads possessed biological activity, only soy bread crust extract reduced PC-3 cell proliferation. This observation may be related to the presence of soy in this bread.     

Antiproliferative Activity and Apoptosis Induction of Eucalyptus Citriodora Resin and Its Major Bioactive Compound in Melanoma B16F10 Cells

Antiproliferative activity and apoptosis induction of ethyl acetate of Eucalyptus citriodora resin (EAEER), and its major bioactive compound in melanoma B16F10 cells were investigated. 6-[1-(p-Hydroxy-phenyl)ethyl]-7-O-methyl aromadendrin (HEMA), a flavanol derivative, was isolated from EAEER and identified on the basis of its mass and NMR spectra. The results from MTT assay showed high antiproliferative effects of EAEER and HEMA on B16F10 cells. Moreover, EAEER- and HEMA-induced cell apoptosis was association with the decrease in the mitochondrial transmembrane potentials (Δψ(m)), increase in Bax/Bcl-2 ratio, and activation of caspase-3. Cells treated with EAEER and HEMA generated intracellular reactive oxygen species (ROS) and nitric oxide (NO), indicating that ROS and RNS play important roles in the induction of apoptosis in B16F10 cells. Taken together, EAEER and its major bioactive compound, HEMA, inhibited the proliferation of B16F10 cells via apoptosis and may be a potential antimelanoma agent.     

1980－2005年太湖地区土地利用变化及驱动因素分析

为了定量化研究土地利用变化特征及其驱动因素,该文应用遥感和GIS技术,分析江苏省环太湖地区1980-1990年、1990-2000年、2000-2005年3个不同时段土地利用的数量变化特征及土地利用类型的空间转换特征,并对研究区各时段土地利用变化的驱动因素进行探讨。结果表明:25a江苏省环太湖地区土地利用类型发生了复杂变化,3个不同时段土地利用变化特征各不相同且具有明显的区域差异性;区域土地利用程度持续增加,土地利用仍处于发展期;从土地利用类型转移变化看,建设用地转入的速度远大于转出的速度,有规模增加的趋势,而水田、旱地、林地的转入速度小于转出速度,存在规模减少的趋势。人口数量的增加、工业化、城市化带来的经济发展与土地管理政策等人类活动是江苏省环太湖地区土地利用类型转换的主要驱动因素。尽管近年来江苏省环太湖地区的土地开发利用取得了巨大的经济收益,但部分人类经济活动给区域农业的发展及土地资源的可持续利用带来巨大压力,甚至导致以水田为主的区域耕地资源质量的进一步退化。该研究为江苏省环太湖地区土地资源的可持续利用及人-地关系的协调发展提供科学依据。     

Population size and specific nitrification potential of soil ammonia-oxidizing bacteria under long-term fertilizer management

Population size of soil ammonia-oxidizing bacteria (AOB) was quantified by real-time PCR in a long-term (16 years) field experiment under different fertilizer managements. AOB population sizes in mineral nitrogen-fertilized soils and organic manure-fertilized soil were 10.3 and 3.1 times, respectively, that of the control, while phosphorus and potassium fertilization had no significant effect. On the other hand, the AOB specific nitrification potential (soil nitrification potential per AOB cell) was significantly higher (P < 0.05) in organic manure-fertilized soil than in mineral-fertilized soils and the control, indicating that AOB was likely more metabolically active in organic manure-fertilized soils than in mineral nitrogen-fertilized soils after long-term application.     

Determination of total 14C residues of sarafloxacin in eggs of laying hens

[14C]sarafloxacin was orally administered to six laying hens for five consecutive days. Eggs were collected for 15 days after the initial drug treatment. Egg yolk and egg albumen were separated and assayed for total radioactive residues (TRR) using a combustion oxidizer and scintillation counting techniques. Radioactivity was detected in egg yolk and egg albumen on the second day of dosing and reached a maximum at 24 h after drug withdrawal. Thereafter, the sarafloxacin TRR levels in egg albumen declined rapidly and were undetectable 2 days after the last dose, whereas the levels in egg yolk declined at a much slower rate and were undetectable 7 days after drug withdrawal. In both the egg albumen and yolk, HPLC analysis indicated that the parent sarafloxacin was the major component.     

水稻早衰突变体els-R7954的遗传分析和初步定位

为了深入研究水稻早衰机理,找到有效消除和延缓植株早衰的方法,本研究通过350Gy60Co-γ射线辐照水稻浙恢7954干种子,获得1份特异性水稻早衰突变体。观察该突变体生物学性状,发现苗期生长正常,分蘖末期叶片出现早衰现象,灌浆期整个植株枯黄,早衰现象非常明显,将其暂名为elsR7954(early leaf senescence)。遗传分析和基因定位发现,els-R7954受单隐性核基因控制,位于第2染色体SSR标记RM530和RM3774之间,距RM530约5.0c M。为进一步克隆该基因,丰富叶片早衰的分子机制奠定了一定基础,也为研究水稻早衰,最终提高产量和品质提供可能。     

布鲁、奥斯、陆稻(Oryza sativa L.)亲缘关系的细胞遗传学——Ⅲ.13个亲本及146F_1减数分裂的染色体行为

<正> 水稻种间、亚种间杂交,常出现程度不同的 F_1杂种不育。内在机制可归因于染色体结构杂合性(Yao 等,1958;Henderson 等,1959;Shastry 和 Misra,1961;Demeterio等,1965;Engle 等,1969;Dolores 等,1975;Barrios,1981);不育基因(徐冠仁,1945;冈彦一,1953,1964,1974);或核质互作型不育(新城长友,1969)。张德慈(1976)认     

基于灰色预测模型的温室温湿度系统建模与控制

温室温湿度系统是一个典型的混杂系统(hybrid system,HS),系统的输入包括环境调控设备的开关状态以及可测不可控的外界环境因子扰动输入,包括太阳辐射、室外温度、室外湿度、风速、风向等。针对温室温湿度系统的这种混杂特性,该文提出一种基于切换系统的温室温湿度系统建模与预测控制方法。首先,分别在天窗开启与关闭状态下采用遗忘因子递推最小二乘法(forgetting factor recursive least squares,FFRLS)辨识模型参数,得到系统的2个子系统模型。采用灰色预测GM(1,1)模型预测温室温室度系统中可测不可控的扰动输入。然后,将系统预测控制问题描述为混合整数二次规划问题(mixed integer quadratic problem,MIQP),并通过分支定界法求解。分析了系统在有限时间内的稳定性(finite time stable,FTS)。最后进行了仿真研究,仿真结果表明该文中提出的建模和控制方法是有效的。     

以温度为主控参数的日光温室综合环境控制系统的研制与应用

针对目前日光温室综合环境调控存在的问题，在研究日光温室微生态环境变化机理的基础上，以温度为主元的基本理论，利用动态规划理论，建立了以温度为主参量的日光温室综合环境调控模式，利用8031单片微型计算机实现了对日光温室综合环境的动态优化平衡调控，经济有效地解决了日光温室环境多因素的检测调控难题，取得了良好的社会效益和可观的经济效益。     

苜蓿花叶病毒外壳蛋白基因在白三叶中的表达及转基因植株的抗病性分析

以白三叶(Trifolium repens L.)的两个品种Irrigation和Huia的子叶为转化体，用根癌农杆菌(Agrobacterium tumefaciens)介导法将外源的苜蓿花叶病毒外壳蛋白基因转入白三叶，经过筛选、分化和再生，得到了具有卡拉霉素抗性的再生植株。对这些植株进行PCR、Southern和Northern分析，结果表明，外源目的基因已经整合到白三叶基因组中并且得到了表达。对Northern分析呈阳性的植株进行了抗病性检测，证明表达苜蓿花叶病毒外壳蛋白基因的植株病症减轻，发病率、病情指数及病毒积累量都明显低于对照，有的甚至不表现症状，达到了免疫的程度。