抗草甘膦大豆粕外源DNA在肉鸡体内代谢研究

使用PCR方法,在肉仔鸡胸肌和腿肌中,未检出来源于抗草甘膦的转基因豆粕外源cp4 epsps基因;在盲肠食糜和粪便中却检出外源基因,但出现率很低。这表明,在现有的检测条件下,外源DNA的完整性在肉鸡消化道很大程度被破坏,没有进入鸡肉中。     

A retrospective study of 29 cases of otitis media/interna in dairy calves

Epidemiological data, clinical findings, laboratory data, medical imaging, and outcomes were reviewed in 29 dairy calves with otitis media/interna. Age at admission ranged from 1 to 24 wk. The majority of calves were referred during winter. Clinical signs included drooping ear, ptosis, head tilt, abnormal nystagmus, strabismus, dysphagia, regurgitation, stiff neck, opisthotonos, facial hyperesthesia, and purulent aural discharge. Intranasal endoscopic examination of 5 animals revealed nasopharyngeal collapse in 4. Cerebrospinal fluid (CSF) was abnormal in all of 7 cases. Mycoplasma bovis was cultured from all but 1 case with external ear or tympanic bullae samples (n = 12), and Mycoplasma arginini was cultured from the remaining ear sample. Radiographs of the tympanic bullae were performed in 24 calves, tomodensitometry (CT) in 3 calves and ultrasound in 4 calves. According to medical imaging techniques or necropsy, 69% of the cases were classified as chronic. Mean duration of treatment was 23.3 d. The rate of clinical recovery was 75%.     

Nitrate leaching losses under Miscanthus grass planted on a silty clay loam soil

Abstract. Nitrate leaching under newly planted Miscanthus grass was measured for three years. The crop received either no fertilizer-N or an annual spring application of 60 kg or 120 kg N ha^-1. During three winters soil water was collected from porous cup probes installed 90 cm deep. Nitrate leaching was calculated from the mean drain flow recorded in two drain gauges multiplied by the mean nitrate-N concentration in the soil water solutions collected. In the first year soil water nitrate concentrations were high on all treatments and N losses were 154, 187 and 228 kg ha^-1 respectively on the unfertilized treatment and those that received 60 or 120 kg N ha^-1. Leaching losses in the second and third years were, in turn, 8, 24 and 87 kg ha^-1 and 3, 11 and 30 kg ha^-1 for the unfertilized treatment and for the 60 and 120 kg N ha^-1 treatments respectively. Leaching losses were closer to those recorded under extensively managed grassland than arable land. The large losses in the first year were probably due to the previous agricultural management at the site and excessive inputs of N on the fertilized plots. In the second and third year, lower drainage volumes may also have influenced losses. The results show that Miscanthus , once established, can lead to low levels of nitrate leaching and improved groundwater quality compared with growing arable crops.     

Chaos theory and assessment of forest stakeholder attitudes towards Australian forest policy

The Regional Forest Agreement process has dominated Australian forest policy for the past decade. The RFA process set in place a mechanism by which benchmark conservation values were established for forest ecosystems, whilst addressing the needs of the timber industry. The outcomes of a number of RFA's have been fraught with controversy. Key stakeholder groups have shown disagreement with processes and outcomes of methods employed by government both in establishing conservation reserves and areas allocated to timber harvesting. This research uses non-linear techniques to examine the dynamical behavior in stakeholder responses and to identify patterns of behavior that may lead to prediction of stakeholder responses. The method developed in this research provides a bridge between social sciences and Chaos theory.¹