筋骨草内昆虫蜕皮激素类似物对杨干象的杀虫作用

从不同地点、不同时间采集的多花筋骨草植株不同部位中提取出昆虫蜕皮激素类似物,通过向幼虫人工饲料中添加昆虫蜕皮激素类似物的方法,研究了筋骨草中植物源蜕皮激素对杨干象2龄幼虫的杀虫作用。实验结果表明:(1)在人工饲料中加入1-3 ml的筋骨草提取物,在24天内可以杀死 58%~100%的幼虫;(2)从开花前的筋骨草中提取出的物质的杀虫效果明显优于从花期和开花后的植物中提取出的物质的杀虫作用, 其1 mL、2 mL和3 mL剂量全株提取物的杀虫效果分别达到65.22%、85.07%和98.11%(幼虫校正死亡率);(3)植株的根部提取的昆虫蜕皮激素类似物的杀虫效果要比茎部或叶部提取的好,1 mL、2 mL、3 mL剂量提取物的杀虫效果分别达到100%、98.20%和98.32%;(4)筋骨草提取物的杀虫速度较慢,处理杨干象2龄幼虫16天后杀虫率才超过50%;(5)幼虫取食加有筋骨草提取物的饲料时间长短直接影响幼虫死亡率,16天的取食,幼虫死亡率达到80%以上。筋骨草采集地点和填加植物源蜕皮激素的方式对杀虫效果的影响较小。图3表3参21。     

寄生小蜂对小青×黑杨树皮及杨圆蚧挥发油的行为反应

Four-armed airflow olfactometer was used to determining the foraging behavior ofPteroptrix longgiclava (Girault) (Hymenoptera: Aphelinidae) andEncarsia gigas (Tshumakova) (Hymenoptera: Aphelinidae) to the essential oils which emitted from the healthy bark ofPopulus pseudo-simonii×P. nigra, the infested bark injured byQuadraspidiotus gigas (Thiem & Gerneck), the body and scale of fixed 1st-instar-nymph ofQ. gigas. The results from these experiments showed that the volatile oils produced from the injured bark and from the scale of fixed 1st-instar-nymph had a higher attractive ability to female adults of the two species of wasps. The essential oil produced from the scale of the pest at dosages of 3–7 μL and the essential oil emitted by injured bark at dosages of 5–9 μL had a stronger alluring effect on the host searching behavior ofPteroptrix longgiclava. The essential oil from the body of fixed 1st-instar-nymph ofQ. gigas also had certain effect on the host locating effort ofPteroptrix longgiclava andEncarsia gigas. Those two wasps did not shown any reaction to the essential oil produced by the healthy bark of poplar. Foundation item: This paper was supported by National Natural Science Foundation of China (39970620) and the “TRAPOYT” Biography: CHI De-fu (1962-), male, Ph.D., Professor in Northeast Forestry University, Harbin 150040, P.R. China Responsible editor: Chai Ruihai     

不同代谢能、粗蛋白和粗纤维水平对太湖鹅生长性能和屠宰性能的影响

为探索不同能量、蛋白和粗纤维水平对太湖鹅生长发育和屠宰性能的影响,将3种营养成分在不同饲养阶段均采用单因子设计,分为高、中、低3种水平。育雏期(0~4周龄)粗蛋白为17%、19%和21%;代谢能为10.5、11.0和11.5 MJ/kg;粗纤维为3.5%、5.0%和6.5%。育肥期(5~11周龄)粗蛋白为15.5%、16.5%和17.5%;代谢能为10.00、10.65和11.75 MJ/kg;粗纤维为5.0%、6.5%和8.5%。每种营养成分在分成3种水平时,其它营养成分为中等水平,营养成分相同的3个中等水平组合并为一组。将刚出壳雏鹅随机分为7组,每组3个重复,每个重复100只,公母各半。每组饲喂不同营养水平的日粮,观测各组增重、饲料转化比和屠宰性能。结果表明:在育雏期中等蛋白组增重显著大于其它组(P0.05),低蛋白组饲料转化比显著大于高蛋白组(P0.05);不同代谢能水平增重和饲料转化比差异均不显著(P0.05);不同粗纤维水平增重差异不显著(P0.05),高粗纤维组的饲料转化比显著大于其它组(P0.05)。在育肥期不同蛋白水平增重差异不显著(P0.05),各组间饲料转化比差异均显著(P0.05),低蛋白组最低,中等蛋白组最高;中等代谢能组增重显著高于其它组(P0.05),高代谢能组饲料转化比显著小于低代谢能组(P0.05);不同粗纤维水平的增重和饲料转化比差异均显著(P0.05),且均为中等粗纤维组最好,高粗纤维组最差。屠宰测定结果显示,仅见低能组的胸肌率显著高于高、中能组(P0.05),其它指标差异均不显著(P0.05)。建议太湖鹅育雏期日粮采用19%粗蛋白质、10.50 MJ/kg代谢能和3.5%粗纤维为宜;育肥期日粮采用15.5%粗蛋白、10.65 MJ/kg代谢能和6.5%粗纤维为宜。     

Influence of Delipation on the Energy Metabolism in Pig Parthenogenetically Activated Embryos

This study was designed not only to measure the effect of delipation on the developmental viability of pig parthenogenetically activated (PA) embryos, but also to evaluate the changes of mitochondria DNA (mtDNA), reactive oxygen species (ROS) level, adenosine triphosphate (ATP) content and gene (Acsl3, Acadsb, Acaa2, Glut1) expression level at different stages after delipation. Results showed that no effect was observed on the cleavage ability, but significant lower blastocyst rate was obtained in delipated embryos. Copy number of mtDNA decreased gradually from MII to four‐cell stages and subsequently kept consistent with blastocyst stage both in delipated and control embryos, but the copy number of mtDNA in delipated embryos was similar to that in the control groups no matter at which developmental stage was observed. Both in delipated and control embryos, ATP content progressive decreased from one‐cell to blastocyst stages, while just at one‐cell stage, a significant decrease of ATP level was observed in delipated embryos compared with that of control. The level of ROS increased obviously after delipation at cleavage stage, but no difference was seen at blastocyst stage. Finally, the expression level of genes related to fatty acids beta‐oxidation (Acadsb and Acaa2) was decreased, while the expression level of genes related to glucose metabolism (Glut 1) was upregulated after delipation. In conclusion, the reduction of lipids in pig oocytes will affect the developmental competence of pig PA embryos by disturbed energy metabolism and ROS stress.     

Intramammary infusion of an Enterococcus faecium SF68 preparation promoted the involution of drying off Holstein cows partly related to neutrophil‐associated matrix metalloproteinase 9

A problem for dairy cows following milk stasis is to cope with a high risk of intramammary infection and there is a need to initiate an extensive renewal of secretory modules in mammary glands so that milk production in next lactation may be optimized. We recently reported that ultrasonicated Enterococcus faecium SF68 (SF68) is compatible with cow mammary glands and an enhancer of innate immunity during the immediate post‐milk stasis period. The current study further examines the concomitant effect of ultrasonicated SF68 on mammary tissue remodeling. Four Holstein cows each received intramammary infusions of regular antibiotic dry‐cow formula (positive control) and two different doses of SF68 in different quarters. Analyses of individual quarter secretion samples showed faster neutrophil infiltration, earlier modifications in protein composition, including caseins and lactoferrins, as well as more prompt elevation of the specific unit of 92‐kDa matrix metalloproteinase 9 (MMP9) in SF68‐infused quarters compared to the positive controls. Intramammary infusion of ultrasonicated SF68 seems able to accelerate the regression of mammary synthetic capacity and potentiate the breakdown of glandular extracellular matrix, indicating a more efficient mammary gland involution. Correlation analyses imply that the ability of ultrasonicated SF68 to induce faster neutrophil chemotaxis and the associated MMP9 release is partly responsible.     

基于双变量同化和交叉小波变换的冬小麦单产估测

为进一步提高陕西省关中平原冬小麦产量估测的精度,利用集合卡尔曼滤波算法(EnKF)将CERES-Wheat模型模拟的0～20 cm土壤含水率和叶面积指数(LAI)与遥感观测的条件植被温度指数(VTCI)和LAI进行同化,同时利用交叉小波变换分析冬小麦各生育时期同化VTCI和LAI与产量之间的共振周期,通过计算小波互相关度获得各生育时期同化VTCI和LAI的权重,进而构建基于加权VTCI和LAI的冬小麦单产估测模型。结果表明,在样点尺度,经过EnKF同化的VTCI和LAI能够综合表达模型模拟值和遥感观测值的变化趋势;在区域尺度,无论是否同化,经过交叉小波变换的各生育时期VTCI和LAI分别与产量之间存在特定的共振周期,同时发现,同化有助于对关键生育时期的特征提取;相较于未同化构建的估产模型,经过同化构建的估产模型的归一化均方根误差为13.23%,决定系数为0.50,平均相对误差为10.58%,精度略有提升,且估测产量的分布与统计产量的分布更为一致,因此认为将同化与交叉小波相结合构建的双变量单产估测模型精度更高,可为进一步实现高精度的区域产量估测提供研究基础。     

Event-specific quantitative detection of nine genetically modified maizes using one novel standard reference molecule

With the development of genetically modified organism (GMO) detection techniques, the Polymerase Chain Reaction (PCR) technique has been the mainstay for GMO detection, and real-time PCR is the most effective and important method for GMO quantification. An event-specific detection strategy based on the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene is being developed for its high specificity. This study establishes the event-specific detection methods for TC1507 and CBH351 maizes. In addition, the event-specific TaqMan real-time PCR detection methods for another seven GM maize events (Bt11, Bt176, GA21, MON810, MON863, NK603, and T25) were systematically optimized and developed. In these PCR assays, the fluorescent quencher, TAMRA, was dyed on the T-base of the probe at the internal position to improve the intensity of the fluorescent signal. To overcome the difficulties in obtaining the certified reference materials of these GM maizes, one novel standard reference molecule containing all nine specific integration junction sequences of these GM maizes and the maize endogenous reference gene, zSSIIb, was constructed and used for quantitative analysis. The limits of detection of these methods were 20 copies for these different GM maizes, the limits of quantitation were about 20 copies, and the dynamic ranges for quantification were from 0.05 to 100% in 100 ng of DNA template. Furthermore, nine groups of the mixed maize samples of these nine GM maize events were quantitatively analyzed to evaluate the accuracy and precision. The accuracy expressed as bias varied from 0.67 to 28.00% for the nine tested groups of GM maize samples, and the precision expressed as relative standard deviations was from 0.83 to 26.20%. All of these indicated that the established event-specific real-time PCR detection systems and the reference molecule in this study are suitable for the identification and quantification of these GM maizes.     

亚麻纤维脱胶技术及在复合材料领域的应用与研究进展

亚麻(Linum usitatissimum L.)是一种古老的作物,在人类历史上扮演着重要的角色。为了研究亚麻纤维作为可再生材料在生产生活中的应用,综述了亚麻纤维的生物学特性、亚麻纤维脱胶方法与表面改良技术的最新研究进展及其在复合材料领域中的应用,探讨了亚麻纤维的应用前景,旨在为亚麻相关的研究提供理论基础。     

油松建筑材密度管理图的编制及其应用

针对建筑材目的油松人工林经营措施,探讨不同生长发育阶段油松人工林密度调控下蓄积变化,为森林可持续经营提供科学依据。对河北平泉阳坡中土、阴坡中土和阴坡厚土3种主要立地类型的全生命周期油松人工林编制密度管理图,得出培育小、中、大径级建筑材措施。结果表明:河北油松建筑材林的培育,阳坡中土培育小径材、阴坡中土和阴坡厚土培育中大径材,期间经过2~3次间伐以达到高效培育的目的。阳坡中土林分,初植密度3 000~3 300株/hm2,10年生间伐强度12%,15年生强度20%,25年生强度25%,主伐时树高级为9~11 m,培育小径材。阴坡中土林分,初植密度2 700~3 000株/hm2,9年生间伐强度25%,19年生强度30%,主伐时树高级为9~11 m,培育中径材;初植密度2 200~2 500株/hm2,9年生间伐强度20%,17年生强度25%,27年生强度30%,主伐时树高级为11~13 m,培育大径材。阴坡厚土林分,初植密度2 000~2 300株/hm2,7年生间伐强度20%,15年生强度30%,主伐时树高级为11~13 m,培育中径材;初植密度1 000~1 200株/hm2,7年生间伐强度20%,13年生强度20%,23年生强度25%,主伐时树高级为13~15 m,培育大径材。     

Optimal dietary protein requirement for juvenile ivory shell, Babylonia areolate

A growth experiment was conducted to determine the optimal dietary protein requirement for juvenile ivory shell reared in indoor aerated aquaria. Six isoenergetic experimental diets using fish meal, casein and gelatin as protein sources were formulated to contain graded levels of protein (27, 33, 38, 43, 49 and 54% of dry diet, respectively). Triplicate groups of 40 shells (average weight 93.50 ± 1.70 mg) were stocked in 120-l tanks and fed to apparent satiation twice daily for 8 weeks. The results showed that the growth performance and feed utilization were significantly affected by dietary protein level (P < 0.05). Maximum weight gain, mean protein gain, specific growth rate and soft body to shell ratio occurred at 43% dietary protein level (P < 0.05). There were significant differences in protein, lipid, moisture and ash content in soft body; except that ash content in shell was not significantly affected by dietary protein level. Pepsin activity in soft body tissue significantly increased with dietary protein level up to 43%, and trypsin-like enzyme activity increased with dietary protein level up to 49%. However, lipase activity in soft body decreased with increasing dietary protein level. However, no significant differences (P < 0.05) in survival, calcium, phosphorus concentration in the shell and soft body were found among dietary treatments. Quadratic regression analysis of weight gain against dietary protein level indicated that the optimal dietary protein requirement for maximum growth and feed utilization of juvenile ivory shell is 45% of dry diet.