KDY型输油泵运行故障分析及改进措施

针对KDY型输油泵运行中存在的润滑油泄漏、轴窜动量大、泵腰部拆卸不便、易损坏轴承和轴瓦等缺陷进行了原因分析,对泵的原设计提出了相应的改进措施。应用效果表明,改进后的KDY型输油泵的泵效已由原来的65%上升至80%,月润滑油泄漏量降至4kg,延长了止推轴承和轴瓦的使用寿命。     

Effect of testosterone on atherosclerosis in rabbits

AIM: To investigate the action of testoster one on atherosclerosis in a rabbit model. METHODS: 37 male cholesterol-fed rabbits were divided into five groups: castration group: castrated rabbits without exogenous testosterone admin istration; testosterone Ⅰ group: castrated rabbits with exogenous testosterone 0.25 mg·kg-1·d-1; testosterone Ⅱ group: castrated rabbits with e xogenous testosterone 2.5 mg·kg-1·d-1; testosterone Ⅲ group: cas trated rabbits with exogenous testosterone 12.5 mg·kg-1·d-1. The sham operation group was also set. Three months later, the levels of testosteron e, blood lipids (including TG, HDL-C, LDL-C), PAI activity, nitric oxide (NO) co ntent, endothelin (ET), angiotensin Ⅱ (AngⅡ) in blood were detected. RESULTS: It showed that testosterone in castration group was the lowest. There was no significant difference of TG or LDL-C between castration g roup and the other four groups. HDL-C in castration group was lower than that in other four groups. NO content of castration group was lower than that in others , but PAI activity, ET and AngⅡ concentration were higher than that in other gr oups. CONCLUSION:Testosterone is a protective factor against atherosc lerosis in male rabbits.     

高校贫困生心理问题状态及对策

本文分析了高校平困生的心理问题,提出了具体的解决方法.     

苏尼特羊肉食用品质的研究

本研究以不同月龄苏尼特羊为对象,取4个部位肌肉,测定其品质指标,并以小尾寒羊肉对照,结果表明:品种因素对肌肉的剪切值、熟肉率、失水率、蛋白质含量、肌纤维直径、密度的影响显著,其差异显著(p<0.001)但对色泽、胆固醇含量、无显著影响.年龄因素对苏尼特羊肌肉的剪切值、色泽A值(红度)、熟肉率、失水率、蛋白质含量、胆固醇含量、肌纤维直径、密度有显著的影响(p<0.001).部位因素对剪切值;色泽L值和a值、失水率、熟肉率、蛋白质含量、肌纤维直径、密度的影响显著(p<0.001).试验证明苏尼特羊肉的色泽、保水性能、蛋白质含量、熟肉率、组织学特性均优于小尾寒羊.     

日本甜柿开花习性的调查研究

为了给日本甜柿栽培技术提供理论依据和制定科学的管理措施提供参考,以提高贵州柿树的管理水平。连续3年(2012—2014)对日本甜柿开花习性进行了调查研究。结果表明:结果母枝直径直接影响不同大小直径结果母枝的百分率,直径以7.1~8.0mm为最高,其次是直径为6.1~7.0mm,再次是直接5.1~6.0mm;结果母枝直径也直接影响结果母枝萌芽率、开花枝长度、直径、花节位百分率、花序种类、花序量、花种类和花量等内容,以处理4(直径为6.1mm≤d7.0mm)的效果最好,其次是处理3(直径为5.1mm≤d6.0mm)的效果,再次是处理5(直径为7.1mm≤d8.0mm)的效果;结果母枝长度直接影响结果母枝萌芽率、结果母枝抽开花枝长度、直径、花节位百分率、抽开花枝开一顶花两侧花花序量、一顶花一侧花花序量、一顶花花序量、花序总量、开顶花量、侧花量和总花量等内容,结果母枝越长,其效果越好;结果母枝部位直接影响结果母枝抽开花枝长度、直径、花节位百分率、抽开花枝开一顶花两侧花花序量、一顶花一侧花花序量、一顶花花序量、花序总量、开顶花量、侧花量和总花量等内容,在结果母枝的越上部,其效果越好。培养结果母蔓直径大小时,以直径6.1~7.0mm为主,配合直径为5.1~6.0mm和7.1~8.0mm;培养结果母枝长度以长的结果母枝为主,配合较长的结果母枝;结果母枝着生部位,以母枝顶芽结果母枝为主,配合次芽结果母枝。     

Oxidative stress-activated JNK-MAPK signaling pathway is involved in fluctuant high glucose-induced injury of hepatocytes

AIM: To explore the mechanisms of fluctuant high blood glucose-induced apoptosis of hepatocytes. METHODS: SD rats were randomly divided into normal control group (N), stable high blood glucose group (S), fluctuant high blood glucose group (F) and insulin group (I). Diabetic rats were induced by intraperitoneal injection of streptozotocin (65 mg/kg), and the fluctuant high blood glucose animal model was induced by intraperitoneal injection of ordinary insulin and glucose at different time points every day. The blood glucose fluctuation patterns of the animals in F group within 12 weeks were similar every day and no significant difference of the HbA1c concentration was observed compared with S group, indicating that the fluctuant hyperglycemia was successfully established in F group. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the content of malondialdehyde (MDA) and nitric oxide (NO) in the homogenate of the liver tissues were detected by colorimetry. The mRNA and protein levels of JNK, p-JNK, Bax and Bcl-2 were examined by RT-PCR and Western blot. RESULTS: After 12 weeks, the increases in the intakes of food and water, the urine output, and the abnormal liver function were observed in S group, I group and F group. Compared with N group, the MDA level was increased, the content of NO and the activity of SOD and GSH-Px were decreased, and up-regulation of JNK mRNA and p-JNK and Bax proteins, and down-regulation of Bcl-2 were also found in S group, I group and F group. The above effects were more obviously showed in F group. CONCLUSION: Oxidative stress activates JNK-MAPK signaling pathway, which is involved in fluctuant high glucose-induced apoptosis of hepatocytes.     

利用同位素~(32)P示踪技术测定西伯利亚红松接穗与砧木适应性和亲和性的研究

利用同位素32P示踪技术,研究嫁接后西伯利亚红松树体内营养的分配、吸收与运输情况,评价接穗与砧木适应性和亲和性。结果表明:嫁接树的营养吸收明显大于非嫁接树,油松-西伯利亚红松嫁接树营养物质交换最好,是适应性较强的组合,这与引种组长期嫁接试验的生长势观察记录相一致。     

Effect of rhynchophylline on TGF-β1/Smad pathway for processing ventricular remodeling in spontaneously hypertensive rats

AIM: To investigate the effect of rhynchophylline (Rhy) on blood pressure, cardiac hypertrophy and myocardial fibrosis in spontaneously hypertensive rats (SHR). METHODS: Spontaneously hypertensive rats were randomly divided into model group, high dose (10 mg·kg^-1·d^-1) and low dose (2.5 mg·kg^-1·d^-1) group of rhynchophylline, captopril group (17.5 mg·kg^-1·d^-1). Wistar-Kyoto rats were used as normal control. Respectively, systolic blood pressure was measured by tail cuff every 2 weeks. After 10 weeks, heart weight index and left ventricular weight index were calculated. The myocardial hydroxyproline and plasma angiotensin Ⅱ were detected. Moreover, basic myocardial histopathological changes and myocardial collagen fibres were observed by HE staining and Masson staining, respectively. The protein expression of TGF-β₁ and Smad3 in the myocardium was measured by the methods of immunohistochemistry and Western blot. RESULTS: Compared with SHR model group, Rhy significantly reduced blood pressure (P<0.05), the levels of HYP in the myocardium (P<0.05) and the levels of AngⅡ in the plasma (P<0.01). The pathological damages of the myocardial tissues and collagen deposition were attenuated. The protein expression of TGF-β₁ and Smad3 was significantly reduced by the treatment with Rhy (P<0.01). CONCLUSION: Rhynchophylline reduces blood pressure and adjusts to improve ventricular remodeling of SHR. The mechanism may be involved in the TGF-β₁/Smad pathway and reducing AngⅡ content.     

Expression of Jagged2/Notch3 signaling molecules in pulmonary hypertensive rats induced by monocrotaline

AIM: To study the expression of Jagged2/Notch3 signaling molecules in pulmonary vascular wall of pulmonary hypertensive rats induced by monocrotaline. METHODS: SD rats were randomly divided into normal control group (C group,n=15), solvent control group (S group,n=15) and monocrotaline model groups (M group,n=15). The model of pulmonary hypertension was established by a single subcutaneous injection of monocrotaline (50 mg/kg). The rats in S group were given a single subcutaneous injection of the same dose of solvent. After 4 weeks, the pulmonary vascular remodeling was assessed by HE staining, and the mean pulmonary artery pressure (mPAP) and right ventricular systolic pressure (RVSP) were determined by right heart catheterization. The expression of Jagged2/Notch3/Hes5 molecules in the pulmonary vascular wall was detected by immunohistochemical method and real-time PCR. RESULTS: Compared with S group and C group, the percentage of medial wall thickness of smaller arteries in model group increased significantly (P<0.01). The levels of mPAP and RVSP in M group were significantly higher than those in S group and C groups (P<0.01). The results of real-time PCR showed that the expression of Jagged2, Notch3 and Hes5 was significantly increased in M group compared with S group and C group. The data from immunohistochemical detection indicated that Jagged2 mainly expressed in the intima of small lung artery, Notch3 and Hes5 mainly expressed in the medial smooth muscle cells. Compared with S group and C group, the expression of Jagged2 and Notch3 was significantly increased in the lung small arteries of M group. CONCLUSION: The activation of Jagged2/Notch3 signaling pathway might play an important role in the formation of pulmonary hypertension.     

红景天苷提取工艺研究进展

红景天苷是景天科红景天属植物中广泛存在的酚类化合物,具有抗缺氧、抗辐射以及抗癌等药理作用,可用于一些心血管疾病和肝病的预防和治疗。同时红景天苷也可用作保健食品,具有滋补强身、抗氧化、抗疲劳、提高体力、延缓肌体衰老等功能。现对红景天属植物中红景天苷的提取工艺进行了综述,并简要阐述了各方法的优缺点,以期为深入研究红景天苷提取工艺提供理论基础。